全文获取类型
收费全文 | 81篇 |
免费 | 10篇 |
专业分类
林业 | 3篇 |
农学 | 2篇 |
4篇 | |
综合类 | 34篇 |
农作物 | 5篇 |
水产渔业 | 18篇 |
畜牧兽医 | 17篇 |
园艺 | 2篇 |
植物保护 | 6篇 |
出版年
2024年 | 2篇 |
2023年 | 4篇 |
2022年 | 3篇 |
2021年 | 3篇 |
2020年 | 3篇 |
2019年 | 2篇 |
2018年 | 4篇 |
2017年 | 2篇 |
2016年 | 2篇 |
2015年 | 7篇 |
2014年 | 2篇 |
2013年 | 1篇 |
2012年 | 2篇 |
2011年 | 8篇 |
2010年 | 20篇 |
2009年 | 1篇 |
2008年 | 3篇 |
2007年 | 3篇 |
2004年 | 3篇 |
2003年 | 1篇 |
2001年 | 1篇 |
1997年 | 1篇 |
1994年 | 1篇 |
1993年 | 3篇 |
1992年 | 3篇 |
1991年 | 2篇 |
1990年 | 1篇 |
1987年 | 1篇 |
1943年 | 1篇 |
1924年 | 1篇 |
排序方式: 共有91条查询结果,搜索用时 31 毫秒
1.
Marine Ollier Vincent Talle Anne‐Laure Brisset Zo Le Bihan Simon Duerr Marc Lemmens Ellen Goudemand Olivier Robert Jean‐Louis Hilbert Hermann Buerstmayr 《Plant Breeding》2019,138(1):69-81
Fusarium head blight (FHB) is a cereal disease of major importance responsible for yield losses and mycotoxin contaminations in grains. Here, we introduce a new measurement approach to quantify FHB severity on grains based on the evaluation of the whitened kernel surface (WKS) using digital image analysis. The applicability of WKS was assessed on two bread wheat and one triticale grain sample sets (265 samples). Pearson correlation coefficients between Fusarium‐damaged kernels (FDK) and WKS range from r = 0.77 to r = 0.81 and from r = 0.61 to r = 0.86 for the correlation between deoxynivalenol (DON) content and WKS. This new scoring method facilitates fast and reliable assessment of the resistance to kernel infection and shows significant correlation with mycotoxin content. WKS can be automated and does not suffer from the “human factor” inherent to visual scorings. As a low‐cost and fast approach, this method appears particularly attractive for breeding and genetic analysis of FHB resistance where typically large numbers of experimental lines need to be evaluated, and for which WKS is suggested as an alternative to visual FDK scorings. 相似文献
2.
Marine Rolland Bodil K. Larsen Jørgen Holm Johanne Dalsgaard Peter V. Skov 《Aquaculture International》2015,23(4):1071-1087
3.
Antoine Leduc Sylvain Souchet Marine Gel Fabienne Le Provost Marion Boutinaud 《Journal of animal science》2021,99(7)
In the dairy cow, negative energy balance affects milk yield and composition as well as animal health. Studying the effects of negative energy balance on dairy cow milk production is thus essential. Feed restriction (FR) experiments attempting to reproduce negative energy balance by reducing the quantity or quality of the diet were conducted in order to better describe the animal physiology changes. The study of FR is also of interest since with climate change issues, cows may be increasingly faced with periods of drought leading to a shortage of forages. The aim of this article is to review the effects of FR during lactation in dairy cows to obtain a better understanding of metabolism changes and how it affects mammary gland activity and milk production and composition. A total of 41 papers studying FR in lactating cows were used to investigate physiological changes induced by these protocols. FR protocols affect the entire animal metabolism as indicated by changes in blood metabolites such as a decrease in glucose concentration and an increase in non-esterified fatty acid or β-hydroxybutyrate concentrations; hormonal regulations such as a decrease in insulin and insulin-like growth factor I or an increase in growth hormone concentrations. These variations indicated a mobilization of body reserve in most studies. FR also affects mammary gland activity through changes in gene expression and could affect mammary cell turnover through cell apoptosis, cell proliferation, and exfoliation of mammary epithelial cells into milk. Because of modifications of the mammary gland and general metabolism, FR decreases milk production and can affect milk composition with decreased lactose and protein concentrations and increased fat concentration. These effects, however, can vary widely depending on the type of restriction, its duration and intensity, or the stage of lactation in which it takes place. Finally, to avoid yield loss and metabolic disorders, it is important to identify reliable biomarkers to monitor energy balance. 相似文献
4.
Hunter J. Piegols Brittany E. Abrams Janis M. Lapsley Megan T. Cray Josephine A. Dornbusch Christina Murphy Brandan G. Wustefeld-Janssens Carlos H. Souza Marine Traverson Pierre Amsellem Elroy Williams Owen T. Skinner Julius M. Liptak Julie A. Stephens Laura E. Selmic 《Veterinary and comparative oncology》2023,21(4):673-684
Adrenalectomies for canine adrenal tumours are associated with peri-operative morbidity and mortality. Objectives of this study included assessing the prognostic value of tumour- or surgery-related variables in predicting peri-operative mortality and overall survival in dogs undergoing adrenalectomies for primary adrenal tumours as well as pre-treatment with phenoxybenzamine on survival to discharge with pheochromocytomas specifically. A multi-institutional retrospective cohort study was performed across nine institutions. Electronic medical record searches identified 302 dogs which met the inclusion criteria. Data collected included dog-related, tumour-related, treatment-related, surgery-related, and outcome variables. Univariate and multivariable logistic regression and cox proportional hazards models were used to identify variables associated with death prior to discharge and tumour-related survival. Overall, 87% of dogs survived to discharge with a tumour-related survival time of 3.96 years. Post-operative complications were reported in 25%. Increased surgical time (p = 0.002) and pre-surgical medical treatment other than phenoxybenzamine (p = 0.024) were significantly associated with increased peri-operative mortality while ureteronephrectomy (p = 0.021), post-operative pancreatitis (p = 0.025), and post-operative aspiration pneumonia (p < 0.001) were significantly associated with decreased overall survival. Phenoxybenzamine pretreatment had no effect on peri-operative mortality. Thirty-seven of 45 (82%) dogs with pheochromocytomas not pretreated survived to discharge, and 50 of 59 (85%) dogs with pheochromocytomas pretreated with phenoxybenzamine survived to discharge (p = 0.730). This study provides information on risk factors for death prior to discharge and tumour-related survival that may help guide clinical management and owner expectations. In addition, the study findings challenge the previously reported benefit of phenoxybenzamine for pretreatment of dogs undergoing adrenalectomies for pheochromocytomas. 相似文献
5.
6.
Aurélie Bourguet Virginie Piccicuto Elise Donzel Marine Carlus Sabine Chahory 《Veterinary ophthalmology》2015,18(4):345-349
This report describes the clinical presentation, diagnosis, histological lesions, and prognosis of a primary choroidal malignant melanoma in a 15‐year‐old cat. The animal was presented for unilateral blindness. On ocular examination, a raised pigmented mass protruding from the posterior pole into the vitreous body was observed by diffuse transillumination and indirect ophthalmoscopy. Ocular ultrasound and computer tomography (CT) scan confirmed localization of the tumor to the posterior segment. The diagnosis of primary choroidal melanoma was confirmed by histopathology after enucleation. To our knowledge, this is the first reported case of a feline malignant melanoma with a primary choroidal localization without iris involvement. 相似文献
7.
Christophe Lambert Philippe Soudant Marine Jegaden Maryse Delaporte Yannick Labreuche Jeanne Moal Jean-Franois Samain 《Aquaculture (Amsterdam, Netherlands)》2007,270(1-4):413-421
Bivalve hemocyte competence has been measured by quantifying functional characteristics, including reactive oxygen intermediate (ROI) production after activation with zymosan or phorbol myristate acetate (PMA). However, untreated oyster hemocytes also produce ROI and RNI (reactive nitrogen intermediates) after bleeding even if not stimulated by zymosan or PMA. Extensive investigation of this parameter by flow cytometry showed that, in vitro, ROI/RNI production by untreated hemocytes maintained in seawater appeared to be independent of both bacterial burden in the serum and non-self particle phagocytosis. ROI/RNI production in granulocytes was higher than in hyalinocytes and could be intensified when activated by zymosan but not by PMA. Both cell types used NADPH-oxidase- and NO-synthase-like pathways to produce these molecules; the NO-synthase pathway seemed relatively more dominant in hyalinocytes and NADPH-oxidase appeared more effective in granulocytes. These results provide new insights for interpreting the modulation of ROI/RNI production by untreated hemocytes shown by other studies, relative to environmental conditions or physiological status of the oysters. 相似文献
8.
9.
大菱鲆抗菌肽基因酵母表达载体的构建及表达 总被引:2,自引:0,他引:2
将大菱鲆(Scophthalmus maxinus)hepcidin基因成熟肽序列经过部分改造后采用PCR方法克隆到毕赤酵母胞内表达载体pGAPZB中,构建了重组胞内表达载体pGAPZB—tbhep6his。用BlnI线性化重组质粒后,电击转化毕赤酵母SMD1168。经过Zeocin筛选和PCR扩增转化子基因组筛选,得到了数株基因工程酵母菌。通过SDS—PAGE和Western blot鉴定,发现目的蛋白与预期蛋白的分子量相吻合,并能与特异抗体antihis特异性结合,表明大菱鲆抗菌肽hepcidin基因在酵母菌中进行了表达。大菱鲆抗菌肽酵母表达载体的构建和表达为进一步研究该抗菌肽的功能并开发新型鱼类饵料奠定了理论基础。 相似文献
10.
Marine Dodet Catherine Collet Henri Frochot L��on Wehrlen 《European Journal of Forest Research》2011,130(1):41-53
By increasing resource availability, canopy opening enhances tree recruitment as well as the development of neighbouring vegetation. The proliferation of early successional and highly competitive vegetation may have dramatic consequences on seedling establishment. However, differences in competitive abilities have been shown among the plant growth forms commonly encountered in forests. We may thus expect that vegetation management leading to control of different plant growth forms would have different consequences on tree seedling growth and development. To test this hypothesis, we analysed the effects of an intensity gradient of four vegetation control treatments (untreated, coppice control, coppice and non-tree plant control, and coppice, non-tree and pioneer (tree) plant control) on plant species richness and natural tree regeneration in three post-storm sites. Higher plant species richness and a better natural tree regeneration were observed in the more intensive treatments that significantly improved the balance of the relative abundance of tree species. Suppressing the more competitive vegetation, mainly Rubus fruticosus and graminoids, led to the recruitment and growth of tree species sensitive to competition and good tree species diversity. Practical recommendations in terms of vegetation control relative to tree regeneration and plant species richness are given. 相似文献