全文获取类型
收费全文 | 353篇 |
免费 | 45篇 |
专业分类
林业 | 8篇 |
农学 | 9篇 |
基础科学 | 1篇 |
60篇 | |
综合类 | 55篇 |
农作物 | 52篇 |
水产渔业 | 12篇 |
畜牧兽医 | 186篇 |
园艺 | 4篇 |
植物保护 | 11篇 |
出版年
2023年 | 4篇 |
2021年 | 7篇 |
2020年 | 8篇 |
2019年 | 8篇 |
2018年 | 5篇 |
2017年 | 7篇 |
2016年 | 7篇 |
2015年 | 11篇 |
2014年 | 7篇 |
2013年 | 16篇 |
2012年 | 11篇 |
2011年 | 16篇 |
2010年 | 7篇 |
2009年 | 5篇 |
2008年 | 3篇 |
2007年 | 19篇 |
2006年 | 8篇 |
2005年 | 8篇 |
2004年 | 13篇 |
2003年 | 14篇 |
2002年 | 12篇 |
2001年 | 11篇 |
2000年 | 9篇 |
1999年 | 6篇 |
1998年 | 6篇 |
1997年 | 3篇 |
1996年 | 4篇 |
1995年 | 6篇 |
1994年 | 6篇 |
1993年 | 7篇 |
1992年 | 8篇 |
1991年 | 15篇 |
1990年 | 10篇 |
1989年 | 5篇 |
1988年 | 11篇 |
1987年 | 8篇 |
1986年 | 12篇 |
1985年 | 7篇 |
1984年 | 7篇 |
1983年 | 9篇 |
1980年 | 5篇 |
1978年 | 4篇 |
1977年 | 3篇 |
1975年 | 3篇 |
1972年 | 3篇 |
1970年 | 6篇 |
1968年 | 3篇 |
1967年 | 2篇 |
1965年 | 2篇 |
1955年 | 2篇 |
排序方式: 共有398条查询结果,搜索用时 328 毫秒
1.
2.
The kinetics-based enzyme-linked immunosorbent assay for coronavirus antibodies in cats: calibration to the indirect immunofluorescence assay and computerized standardization of results through normalization to control values. 总被引:3,自引:0,他引:3 下载免费PDF全文
J E Barlough R H Jacobson D R Downing T J Lynch F W Scott 《Canadian journal of veterinary research》1987,51(1):56-59
The computer-assisted, kinetics-based enzyme-linked immunosorbent assay for coronavirus antibodies in cats was calibrated to the conventional indirect immunofluorescence assay by linear regression analysis and computerized interpolation (generation of "immunofluorescence assay-equivalent" titers). Procedures were developed for normalization and standardization of kinetics-based enzyme-linked immunosorbent assay results through incorporation of five different control sera of predetermined ("expected") titer in daily runs. When used with such sera and with computer assistance, the kinetics-based enzyme-linked immunosorbent assay minimized both within-run and between-run variability while allowing also for efficient data reduction and statistical analysis and reporting of results. 相似文献
3.
4.
5.
6.
7.
8.
Interactions between different media and follicle‐stimulating hormone supplementation on in vitro culture of preantral follicles enclosed in ovarian tissue derived from collared peccaries (Pecari tajacu Linneaus, 1758) 下载免费PDF全文
GL Lima VB Luz LF Lima RMP Rocha SV Castro TS Castelo APR Rodrigues JR Figueiredo AR Silva 《Reproduction in domestic animals》2018,53(4):880-888
The aim was to verify the effect of follicle‐stimulating hormone (FSH) supplementation to α‐MEM+ or TCM199+ media on the in vitro development of ovarian preantral follicles (PFs) derived from collared peccaries. Ovaries (n = 5 pairs) were collected and divided into fragments destined to control group (non‐cultured) or treatments that were cultured for 7 days. The PFs morphology, growth and activation were evaluated by classical histology. The immunohistochemistry markers Ag‐NOR and PCNA were used for nuclear proliferation analysis, and the picrosirius red labelling was used for ovarian extracellular matrix (ECM) evaluation. After 7‐day culture, only the TCM199+ treatment maintained the proportion of intact PFs similar to day 1 (63.2%), but no differences were found among treatments (p > .05). In addition, a significant increase in the growing follicles proportion was verified for all the treatments, indicating follicular activation (p > .05). By the Ag‐NOR analysis, only the TCM199+/FSH maintained the nuclear proliferation similar to the first day (p > .05). The picrosirius red staining revealed that the ECM remained intact in all the treatments (p > .05). We suggest the use of TCM199+ medium supplemented of FSH for the in vitro development of peccaries PFs under 7‐day culturing conditions. 相似文献
9.