菜豆萎蔫病菌的血清检测鉴定技术研究

本研究对我国一类检疫性有害生物菜豆萎蔫病菌（Ｃｕｒｔｏｂａｃｔｅｒｉｕｍｆｌａｃｕｍｆａ－ｃｉｅｎｓｐｖ．ｆｌａｃｕｍｆａｃｉｅｎｓ，Ｃｆ）进行血清检测鉴定技术的研究。试验中制备了抗Ｃｆ的血清，应用抗００２０，０２３７，０２３８菌株的抗血清及其ＦＩＴＣ标记的抗体进行间接和直接免疫荧光染色试验（ＩＦ／ＩＦ）。通过对１９个Ｃｆ菌株和其它６个属１４个种的植物病原细菌２０个菌株的测定，结果发现约５０％的Ｃｆ菌株为强阳性反应，且与Ｃｆ种下致病变种也有强阳性交叉反应。由于单个血清检测易产生假阴性反应，故将三种血清１∶１∶１混合后进行ＩＦ试验，结果表明与所有的Ｃｆ菌株反应，且与Ｒｆａ和Ｃｍｔ有弱阳性反应。对含１０４～１０７ｃｆｕ／ｍｌ细菌及３０ｇ种子含１粒人工接种种子的种子抽提液均能检测到典型的阳性细胞，血清检测的灵敏度为４．５×１０４ｃｆｕ／ｍｌ，可用于种子的检测。本研究还进行了初步的免疫荧光菌落染色（ＩＦＣ）试验     

Contagious bovine pleuropneumonia within the 19th century miasmatic landscape

When contagious bovine pleuropneumonia (CBPP) was first detected on a farm north of Melbourne, at Bundoora, in 1858, the predominant theory of miasma was being challenged by contagionist theories of disease transmission. This well‐documented case was recorded during a period of change in the scientific assessment of disease and therefore affords an exploration of what aspects of the landscape were considered important for livestock health at the time. Although the introduction, vaccination programs and eventual eradication of CBPP on mainland Australia has been well explored, scholars have neglected this aspect of the disease's history. By comparing 19th century records of farmland with how the site appears today, it is also possible to highlight the limited information provided by contemporary texts, while at the same time developing an appreciation of the ways in which the perception of the rural landscape has changed. This differing perception has implications for the utilisation of these sources for veterinary and environmental historians seeking to understand the mid‐19th century agricultural landscape and how it relates to animal health.     

高校数字图书馆著作权合理使用的相关问题

数字技术和网络环境改变了高校图书馆的传统服务模式,同时也给著作权合理使用制度带来新的挑战。探讨在网络环境下,如何正确对待及适应国家乃至国际上对著作权的相关法律规定。完善著作权在高棱数字图书馆的合理使用。     

中国蝗虫学研究60年

蝗虫灾害是人类历史上三大自然灾害之一,对农牧业、环境和经济构成严重威胁。我国是世界上遭受蝗灾最严重的国家之一,有关蝗虫学的研究有较长的历史。60年前,通过改治结合,我国控制了大规模的飞蝗Locusta migratoria蝗灾发生,取得了举世瞩目的成就。60年过去了,中国蝗虫学研究发生了翻天覆地的变化。该文主要综述了最近60年中国蝗虫学研究的发现与创新,展示蝗虫分类学、生态学、生殖与发育生物学、分子生物学、基因组学研究以及蝗虫控制技术研究等多个方面的成就。这些研究成果是世界昆虫学研究的重要组成部分。特别是近30年,我国科学家深入揭示蝗虫两型转变的遗传和表观遗传机制,在国际上产生了重要影响。飞蝗的综合生物学研究积累使飞蝗成为继果蝇之后又一个昆虫模式系统。更重要的是,我国科学家开发的真菌生物农药和群聚信息素可以作为重要的防治手段用于蝗灾的治理。     

Intra-specific variation and inheritance of BYDV-PAV transmission in the aphid <Emphasis Type="Italic">Sitobion avenae</Emphasis>

Vector efficiency of 44 clonal lines (clones) of Sitobion avenae belonging to 31 different genotypes (distinct patterns for five microsatellite loci) originating from Western France was evaluated by transmitting the isolate PAV4 of BYDV-PAV to barley seedlings. Variation in transmission rates from 3.7% to 92.5% was observed, with significant effects of the aphid clone, of the plant species on which clones were collected, and of the reproductive mode of the clones. When genotypes are considered instead of clones, a continuum in transmission rates was observed. A subset of S. avenae clones was tested for transmission of one (10 clones) and 13 (4 clones) other BYDV-PAV isolates, and a clear clone effect modulated by an isolate effect was observed. Crosses were made between clones with different vectoring phenotypes and their F1 progeny were tested for PAV4 transmission. The narrow sense heritability of the PAV transmission character was rather high in the F1 families (h²=0.5) and the segregation analyses suggested an oligo/polygenic determinism of this character. The possibility of generating new transmission variants by sexual reproduction and its consequences on transmission mechanism studies and on BYD epidemics are discussed.     

宁夏荒漠草原植物群落的空间异质性

荒漠草原植被最显著的特点是植物种类贫乏,几乎全部为旱生种类,且空间异质性强,植物与自然环境之间的联系密切,小地形的差异势必会造成植物群落物种多样性的变化及其在不同尺度上的空间异质性。本研究于2011年9月在宁夏盐池县试验区封育10年的荒漠草原围栏内,采用样线取样法在坡面尺度上调查不同坡位上草地群落的空间分异,分析微地形条件对荒漠草原植物群落空间异质性的影响。结果表明,植物群落的空间变异性在不同坡向及不同坡位间存在差异;矮小半灌木功能群在空间上的分布斑块化最明显,多年生草本功能群次之,一年生草本功能群在阴坡上的分布斑块化程度较高,在阳坡上其斑块性明显下降;在草地植物群落恢复演替的进程中,多年生草本植物已发展到相对稳定的程度,矮小半灌木处于增长期,一年生草本植物在阴坡的增长大于阳坡。     

内蒙古红豆草黄萎病菌的分离鉴定及其生物学特性

依照柯赫氏法则,利用形态学结合分子生物学的方法首次对内蒙古自治区呼和浩特市中国农科院草原研究所沙尔沁基地红豆草疑似黄萎病的病样进行分离和鉴定,利用蘸根接种法对病原菌的致病性进行测定.同时,首次在室内条件下对病菌的生物学特性,如生长最适温度、酸碱度以及碳源、氮源对病原菌生长速率和产孢量的影响进行了研究.结果表明,引起沙尔...