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Peixin YANG Mohamed S. MEDAN Koji Y. ARAI Wanzhu JIN Gen WATANABE Kazuyoshi TAYA 《Animal Science Journal》2005,76(5):427-434
The ontogeny of testicular inhibin/activin in ducks was investigated. Testicular localization of three inhibin/activin subunits (α, βA and βB) was determined in embryonic and newly hatched ducks from 12 days of incubation to 1 day of age, in immature ducks and in adult ducks. In the duck embryonic testis, positive α‐subunit immunostaining was first detected in the Leydig cells and Sertoli cells on day 15 of incubation, whereas βA‐subunit and βB‐subunit immunostaining were found in Sertoli cells and primary germ cells on day 18 of incubation. In 1 month old ducks, intense staining of α‐subunit was present in the seminiferous epithelium consistent with localization in Sertoli cells and primary germ cells, and the immunostaining of the βA‐ and βB‐subunit was also present in Sertoli cells and primary germ cells. Specific immunostaining with inhibin/activin α‐, βA‐ and βB‐subunits antisera occurred in Sertoli cells in the adult duck testes. In conclusion, it was shown that, in the duck testis, the majority of α‐, βA‐ and βB‐subunits are colocalized in Sertoli cells with a certain degree of staining in germ cells and the α‐subunit is present in Leydig cells of embryonic testes before day 18 of incubation. These results indicate that Sertoli cells and possibly germ cells in the embryonic testes of late stage of incubation and newly hatched ducks, immature ducks and mature ducks may produce bioactive inhibin dimers, inhibin A and inhibin B, as a possible regulator of follicle‐stimulating hormone secretion. Free inhibin/activin subunits and their dimers may also play an autocrine/paracrine role in the development of the testis and spermatogenesis. Furthermore, early onset of the α‐subunit in duck testes indicates that it may have an autocrine/paracrine effect on steroid hormones, which is important for sex differentiation. 相似文献
4.
Changes of receptor mRNAs for oxytocin and estrogen during the estrous cycle in rat uterus 总被引:1,自引:0,他引:1
Murata T Narita K Honda K Higuchi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(6):707-712
Oxytocin receptor (OTR) mRNA levels increase dramatically near term and is potently stimulated by estrogen because increased OTR mRNA levels result from estrogen treatment in ovariectomized rat uterus. In this study, OTR, estrogen receptor (ER) alpha and ERbeta mRNA levels in the rat uterus during the estrous cycle were examined by quantitative RT-PCR. OTR mRNA levels during the estrous cycle began to increase on diestrus (P<0.05, vs value on estrus), reached maximal increase both in the morning (1000-1130 hr) and afternoon (1600-1630 hr) on proestrus (P<0.01, vs metestrus, diestrus and estrus) and then declined on estrus. In contrast ER alpha mRNA levels began to decrease on diestrus, reached statistical significance both in the morning and the afternoon on proestrus (P<0.01, vs metestrus, diestrus and estrus) and returned to the value of metestrus on estrus. ERbeta mRNA levels were low in the morning and the afternoon on proestrus (P<0.01, vs metestrus and estrus) and also returned to metestrus values on estrus. Treatments with estrogen for 3 days significantly decreased both ERalpha and ERbeta mRNA levels. It can be concluded from these results that during the estrous cycle, OTR mRNA levels in rat uterus predominantly increase at proestrus with a decrease in ERalpha and ERbeta mRNA levels, which is probably due to the increased estrogen levels in circulation before ovulation. 相似文献
5.
Suppressive effect of liquid potassium silicate on powdery mildew of strawberry in soil 总被引:3,自引:0,他引:3
Takeshi Kanto Akihiro Miyoshi Takuya Ogawa Kazumasa Maekawa Masataka Aino 《Journal of General Plant Pathology》2006,72(3):137-142
A soil drench of soluble silicon (liquid potassium silicate) was tested for the control of powdery mildew of strawberry in
soil for 2 years in a vinyl house under growers' conditions on two cultivars: very susceptible Toyonoka and slightly susceptible
Sachinoka. Soluble silicon suppressed the disease more effectively as a preventive control than as a control to reduce initial
incidence. The suppressive effect against this disease was more effective for Toyonoka than for Sachinoka. The control value
of soluble potassium silicate was 85.6% for Toyonoka in the first year and 60.2% in the second year, while for Sachinoka it
was 58% in the first year and 40.6% in the second year. Strawberry leaf hardness was measured for the control and silicate-treated
leaves. Although silicate-treated leaves seemed to be harder than control leaves, leaf hardness did not vary significantly
between controls and silicate-treated leaves in either of the 2 years, and the mode of preventive action of liquid potassium
silicate remains unknown. A factor other than a physical action may be involved. 相似文献
6.
Effects of loosing Aigamo ducks on the growth of rice plants, weeds, and the number of arthropods in paddy fields 总被引:2,自引:0,他引:2
SEISHU TOJO MASAHIRO YOSHIZAWA TAKASHI MOTOBAYASHI KENGO WATANABE 《Weed Biology and Management》2007,7(1):38-43
Aigamo duck farming has been noticed as one of the techniques of environmental conservation agriculture. This study was carried out to investigate the effects of loosing Aigamo ducks on the growth and production of rice plants, the quantity of weeds and arthropods, and the balance of nitrogen (N) in paddy fields by changing the density of the Aigamo ducks. A paddy field was divided into three test plots: a 10-Aigamo-duck plot, a five-Aigamo-duck plot, and a weeding plot. The results show that the height, number of culms, and yield crops of the rice plants increased more in the 10-Aigamo-duck plot and the five-Aigamo-duck plot than that of the weeding plot. Millet ( Echinochloa oryzicola ) remained in both the Aigamo duck plots. The weeding plot was covered with monochoria ( Monochoria vaginalis ). In both the Aigamo duck plots, the outbreak of total arthropods was lower than that of the weeding plot, but the number of rice plants damaged by grass leaf rollers ( Cnaphalocrocis medinalis ) was increased. The number of spiders, regarded as useful insects, did not differ that much between the experimental plots. The N content of the soil did not differ as an effect of loosing the Aigamo ducks. The rice plants in the 10-Aigamo-duck plot absorbed more N than the rice plants in the other plots. 相似文献
7.
Kazumasa Yoshida Mitsuru Nishiguchi Shojiro Hishiyama Atsushi Kato Koetsu Takahashi 《Journal of Wood Science》2006,52(4):372-375
Sugi (Cryptomeria japonica D. Don) produces secondary metabolite norlignans in xylem. Several norlignans are involved in the coloration of heartwood
and defense of sapwood against microbial invasion. Their biosynthetic process should be well understood so that their properties
can be exploited to improve the quality and utility of C. japonica wood. Unfortunately, information on the norlignan biosynthesis is limited because norlignans are mainly synthesized in a
particular season in the transition zone (TZ) along with the heartwood formation, and is difficult to study. Although the
generation of two norlignans of C. japonica, agatharesinol and (E)-hinokiresinol, has been reported, systems for producing other norlignans are not yet known. To establish a novel norlignan
generating system, we examined the changes occurring in norlignans in a TZ during the process of drying a C. japonica log. On the day of felling, the TZ contained agatharesinol and (E)-hinokiresinol, which increased until they reached a maximum on day 40 after felling. Sequirin-C appeared on day 40 and increased
to day 70. The generation of sequirin-C in the TZ can be used to investigate the biosynthetic process of heartwood norlignans.
This study describes for the first time the changes that occur in the composition of norlignan during the drying of the TZ. 相似文献
8.
Haney SAMIR Kazuaki SASAKI Eman AHMED Aly KAREN Kentaro NAGAOKA Mohamed EL SAYED Kazuyoshi TAYA Gen WATANABE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(5):549-556
Although color Doppler ultrasonography has been used to evaluate testicular blood flow in
many species, very little has been done in goat. Eight male Shiba goats were exposed to a
single intramuscular injection of either gonadotropin-releasing hormone (GnRH group; 1
µg/kg BW) or human chorionic gonadotropin (hCG group; 25 IU/kg BW).
Plasma testosterone (T), estradiol (E2) and inhibin (INH) were measured just before (0 hr)
and at different intervals post injection by radioimmunoassay. Testis volume (TV) and
Doppler indices, such as resistive index (RI) and pulsatility index (PI) of the
supratesticular artery, were measured by B-mode and color Doppler ultrasonography,
respectively. The results indicated an increase in testicular blood flow in both groups,
as RI and PI decreased significantly (P<0.05), but this increase was
significant higher and earlier in hCG group (1 hr) than in the GnRH group (2 hr). A high
correlation was found for RI and PI with both T (RI, r= −0.862; PI, r= −0.707) and INH in
the GnRH group (RI, r=0.661; PI, r=0.701). However, a significant
(P<0.05) correlation was found between E2 and both RI (r= −0.610) and
PI (r= −0.763) in hCG group. In addition, TV significantly increased and was highly
correlated with RI in both groups (GnRH, r= −0.718; hCG, r= −0.779). In conclusion, hCG
and GnRH may improve testicular blood flow and TV in Shiba goats. 相似文献
9.
Toyokazu KOBAYASHI Hiroshi KOIE Arisa WATANABE Arisa INO Kazuya WATABE Mujo KIM Kiichi KANAYAMA Kazuya OTSUJI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(4):503-506
We examined the effects of chicken egg hydrolysate (also known as “bone peptide” or BP)
on bone metabolism in 5- to 8-month-old orchidectomized dogs. The bone formation marker
serum bone alkaline phosphatase (BAP) and the bone resorption marker urine
deoxypyridinoline (DPD) were used as indicators to measure changes in bone metabolism. The
following results were observed that Serum BAP was higher in dogs fed BP-enriched food
throughout the clinical investigation. Serum BAP was statistically significantly higher in
dogs fed BP-enriched food than in dogs fed non-BP-enriched food at 2 months after
orchidectomy. This suggests that BP promoted bone formation immediately after
orchidectomy. 相似文献
10.
Kazuhiro WATANABE Kotaro HAYASHI Saku KIJIMA Chie NONAKA Kazuaki YAMAZOE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1323-1325
In this study, scaling, polishing and daily tooth brushing were performed in 20 beagle
dogs, and the number of oral bacteria was determined using a bacterial counter. The dogs
were randomized into the scaling (S), scaling + polishing (SP), scaling + tooth daily
brushing (SB) and scaling + polishing + tooth daily brushing (SPB) groups. Samples were
collected from the buccal surface of the maxillary fourth premolars of the dogs
immediately after scaling and every week thereafter from weeks 1 to 8. Throughout the
study, the number of bacteria was significantly lower in the SB and SPB groups compared
with the S group. The findings suggest that daily tooth brushing inhibited oral bacterial
growth in the dogs. 相似文献