The value of sea urchin,Lytechinus variegatus,egesta consumed by shrimp,Litopenaeus vannamei

Sea urchins produce high‐energy, membrane‐bound fecal pellets that contain residual nutrients and large quantities of microbiota. These egesta are readily consumed by the shrimp, Litopenaeus vannamei. Egesta of the sea urchin, Lytechinus variegatus, were evaluated as a feed supplement or total replacement for a commercial shrimp diet. Shrimp were stocked at 0.49 g ± 0.06 g initial body weight and housed individually in 2.8‐L tanks in a commercial recirculating zebrafish system. Shrimp were assigned to one of six diets: commercial shrimp feed, reference sea urchin feed, collected dried sea urchin egesta, collected wet sea urchin egesta, half ration of shrimp feed and half collected wet sea urchin egesta, and egesta naturally produced by two sea urchins in polyculture. Equivalent dry matter amounts of each diet were proffered to shrimp in each treatment twice daily, except for those that had complete access to natural egesta excreted by sea urchins in polyculture. Sea urchins were proffered a reference sea urchin feed at 2% body weight daily. After 27 days, shrimp proffered collected dried or wet egesta did not differ significantly in percent weight gain and showed the lowest weight gain. The percent weight gain of shrimp fed the commercial shrimp diet did not differ significantly from that of the shrimp fed half commercial shrimp diet and half egesta. The highest weight gain was recorded for those shrimp that consumed the untouched egesta produced by sea urchins in polyculture. These data suggest that consumed egesta have noteworthy nutritional value and therefore would be beneficial to the culture of extractive species in an integrated multitrophic aquaculture system.     

Radioisotope and stable isotope ratios (Δ14C, δ15N) suggest larval lamprey growth is dependent on both fresh and aged organic matter in streams

Lampreys have a complex life cycle which includes a multi‐year infaunal larval stage (ammocoete). Gut content analysis has generally identified detritus (i.e., unidentifiable organic matter) as the major dietary component to ammocoetes, though algae can also be important. However, gut content preserves only a snapshot of the animal's diet and does not reflect assimilated material. In order to better characterise the nutritional sources supporting ammocoete growth, we analysed ammocoete body tissue and potential dietary sources at two streams using natural Δ¹⁴C and δ¹⁵N to estimate time‐integrated nutritional support. Bayesian isotope mixing models revealed differences in the importance of sources supporting ammocoetes between sites. Ammocoetes from a stream in a mixed land usage area (~50% agriculture, ~40% forest and ~10% developed) were primarily supported (mean: ~50%) by fresh terrestrial organic matter but were also supported by substantial contributions (mean: ~30%) by aged organic matter (AOM) and autochthonous material (algae; mean ~20%). In a predominantly forested (~90%) headwater stream, different modelling scenarios (uninformed or informed priors) suggested that algal support of ammocoete nutrition ranged from 7% to 45%. However, the model relying on informed priors developed from gut content analysis produced the low estimates, suggesting these were more reliable. When algae were a minor component of the nutrition at the forested site, ammocoetes were highly dependent on AOM (83 ± 26%; mean ± SD). Based on these findings, ammocoete growth and development are predicted to be strongly influenced by both land use and the availability of allochthonous and autochthonous materials of varying ages within streams.     

Verrucosamide,a Cytotoxic 1,4-Thiazepane-Containing Thiodepsipeptide from a Marine-Derived Actinomycete

A new cytotoxic thiodepsipeptide, verrucosamide (1), was isolated along with the known, related cyclic peptide thiocoraline, from the extract of a marine-derived actinomycete, a Verrucosispora sp., our strain CNX-026. The new peptide, which is composed of two rare seven-membered 1,4-thiazepane rings, was elucidated by a combination of spectral methods and the absolute configuration was determined by a single X-ray diffraction study. Verrucosamide (1) showed moderate cytotoxicity and selectivity in the NCI 60 cell line bioassay. The most susceptible cell lines were MDA-MB-468 breast carcinoma with an LD₅₀ of 1.26 µM, and COLO 205 colon adenocarcinoma with an LD₅₀ of 1.4 µM. Also isolated along with verrucosamide were three small 3-hydroxy(alkoxy)-quinaldic acid derivatives that appear to be products of the same biosynthetic pathway.     

MAGNETIC RESONANCE, ULTRASOUND AND HISTOPATHOLOGIC CORRELATION OF ACUTE AND HEALING EQUINE TENDON INJURIES

Ultrasonography and MRI have become valuable tools for imaging of tendon injuries. The current study examines the histopathologic basis for the imaging abnormalities. Five injured equine forelimbs and two normal contralateral limbs were studied with high resolution real time ultrasound and MRI. Histologic sections were made and correlated with the diagnostic images. All lesions were readily seen by both modalities. Lesions characterized by hemorrhage, edma, and cellular infiltration were sonolucent on ultrasound and bright on MRI images. MRI returned to normal as fibrogenesis ensued. Ultrasound images remained abnormal until fibrillar reallgnment occured with completion of the healing process. High resolution real time ultrasound and MRI both accurately reflect the tissue abnormalities in acute tendon injuries. The injured horse does appear to be an effective model for correlative imaging studies of tendon injuries.     

Beef feedlot industry.

The United States cattle feeding sector has changed dramatically over time. Movement from an industry largely populated by farmer feeders using livestock to market their grain production to an industry composed of large, specialized, commercial cattle feeding firms has occurred since the end of World War II. Moreover, the industry also shifted geographically, moving away from the Corn Belt to the Plains as farmer feeders exited the industry. Today, the 10 largest firms in the industry likely market 22% to as much as 29% of all fed cattle produced in the United States. The shift in industry structure was at least partially motivated by the advent of improved technology. Successful implementation of rapidly evolving technology often required more intense, specialized management (and in some cases, larger operations) than was available in many farmer feeder operations. The ongoing shift away from live-weight pricing of fed cattle toward grid-based pricing could increase the need for specialized management yet again, encouraging even more industry consolidation in the future.     

Quasi-non-linear fracture mechanics analysis of splitting failure in simply supported beams loaded perpendicular to grain by dowel joints

A quasi-non-linear fracture mechanics model based on beam on elastic foundation theory is applied for analysis of the splitting failure of dowel joints loaded perpendicular to grain. Simply supported beams symmetrically loaded by two dowels are considered, and the effects of edge distance, dowel spacing, and distance between dowels and supports are accounted for. The foundation modulus used in the beam on elastic foundation model is chosen so that the perpendicular-to-grain tensile strength and fracture energy properties of the wood are correctly represented. This ensures that a conventional stress analysis and failure criterion lead to the same solution as the compliance method of fracture mechanics. A semiempirical efficiency factor is proposed to account for the influence of the total beam depth, which does not enter the beam on elastic foundation model, but the effect of which is evident from tests. It is shown that the so-called Van der Put/Leijten model, which recently has been adopted in Eurocode 5, appears as a special case of the model presented. Tests on simply supported beams with a single dowel joint at midspan are compared with the theoretical predictions. Various edge distances, beam depths, and spans were tested.     

Artificial Spawning of Tilapia Eggs1

A stripspawning methodology was evaluated for tilapia (oreochromid) species. This technique achieved an average hatching success of 68.6 ± 3.6% (N= 31). Female fecundity and spawning frequency were dependant on both genetical and husbandry factors. Egg yields for Oreochromis niloticus, O. mossambicus, and O. niloticus±O. mossambicus hybrids averaged 4.54, 10.86 and 10.36 eggs/g female/spawn, respectively. Female broodstock that were adapted to an intensive spawning regime exhibited a significant increase in fecundity. Additionally, egg survival was not affected by hydration for up to 15 minutes prior to fertilization. Results suggest that the strip spawning of tilapia species may be an efficient method of providing viable gametes for hatchery purposes.