A toxic dipeptide from the defense glands of the colorado beetle

The Colorado beetle is protected against predators by the secretions of defensive glands located on the pronotum and the elytra. The single major compound of the secretion was identified as gamma-L-glutamyl-L-2-amino-3(Z),5-hexadienoic acid by spectroscopic and chemical methods. This compound, which contains a nonprotein beta,gamma- unsaturated amino acid, is toxic to ants (Myrmica rubra) at a concentration 10(-2) molar, which is less than its estimated concentration in the secretion (1.8 x 10(-1) molar).     

Shoot culture dynamics of six Populus clones

Shoot tips of five genotypically diverse Populus clones, P. alba x P. grandidentata 'Crandon,' P. nigra 'Betulifolia' x P. trichocarpa, P. nigra x P. laurifolia 'Strathglass,' P. maximowiczii x P. trichocarpa 'Androscoggin' and P. deltoides x P. nigra 'Eugenei,' were collected from hardwood cuttings, sterilized,and established in vitro. Stable shoot cultures were obtained from all clones except P. deltoides x P. nigra 'Eugenei'. The four poplar clones that formed stable shoot cultures together with a previously established P. tremula 'Erecta' clone were placed as two-node explants on either Murashige and Skoog medium or Woody Plant Medium containing benzyladenine to determine the rate of shoot multiplication, shoot growth and other responses of the clones. All five poplar clones showed rapid shoot multiplication when cultured in the presence of 0.4-1.0 microM benzyladenine on Murashige and Skoog medium, although P. tremula 'Erecta' produced a greater number of healthy shoots when grown on Woody Plant Medium. Individual shoot growth of all clones was more vigorous when the medium contained 0-0.1 microM benzyladenine, and 100% of such shoots rooted ex vitro.