Effect of soluble glass bolus administration on the blood selenium concentration in grazing Philippine goats under backyard conditions

The present study was conducted to determine the effects of selenium (Se) soluble glass bolus (SGB) on blood Se concentrations of mixed‐bred (native × Anglo Nubian) pregnant does weighing between 15 and 52 kg and grazing on natural pastures in the Philippines. The control group, consisting of 28 does, was not administered with SGB, while 23 does were administered with SGB every 6 months for a period of 12 months. Forage and blood samples in both groups were collected every 2 months. Their Se, sulfur, calcium, phosphorus, magnesium, copper and zinc concentrations were analyzed using the wet digestion method. The results showed that the grazed forage had concentrations of all these minerals that were far higher than the accepted critical lower levels. The Se concentration was higher (P < 0.05) in the dry season than in the rainy season, especially in the whole blood collected from the does and newborn kids. In both groups the Se concentration in the kids tended to increase during the suckling period but decreased after weaning. Kids born to the SGB does had higher whole blood Se concentrations (P < 0.05) than those born to the control does. However, the administration of SGB to the does did not affect any blood mineral concentrations except for Se in the grazing does and their kids. The animals did not exhibit any clinical signs of mineral deficiencies or toxicities during the experimental period.     

Seasonal changes of the mineral contents in the rumen of wild Yeso sika deer (Cervus nippon yesoensis)

The rumen contents were collected from 36 wild Yeso sika deer (Cervus nippon yesoensis) captured by deer culling or by hunting in the spring, summer, autumn and winter in Hokkaido, Japan. Botanical classification was conducted, and the contents of mineral (calcium (Ca), phosphorus (P), potassium (K), sodium (Na), iron (Fe), copper (Cu) and zinc (Zn)) were measured. The animals were captured around pastures or fallow field areas in the Kushiro area. The rumen contents consisted of grasses and Sasa sp. leaves regardless of the season. Leaves and bark were ingested in the spring, autumn and winter. The macro‐mineral contents in the rumen showed seasonal changes. In the summer, the Ca, K and P contents were high, and the Na content was low. There were no seasonal changes in the Fe content. The P, Na and Fe contents were higher than the animals' requirements. In a future survey, it is needed to determine the mineral contents of the food ingested by wild Yeso sika deer.     

Nutritive value of selected grasses in North Sumatra, Indonesia

The nutritive values of seven native grass species collected in North Sumatra, Indonesia, during dry and rainy seasons were evaluated. The chemical composition, in vitro dry matter digestibility (IVDMD), in vitro organic matter digestibility (IVOMD), in vitro crude protein digestibility (IVCPD), macro mineral concentrations of calcium, phosphorus and magnesium, in vitro gas production profile and metabolizable energy (ME) content of the grasses varied greatly among species and seasons. The crude protein content ranged from 6.6 (Andropogon gayanus) to 16.2% dry matter (Cynodon plectostachyus) in the rainy season, with a significant (P < 0.05) reduction in the dry season. Data on the fiber fraction showed that the grasses contained more neutral detergent fiber (NDF) and acid detergent fiber (ADF) in the dry season and it significantly (P < 0.05) decreased in the rainy season, except for Panicum maximum and Pennisetum purpureum for NDF content and C. plectostachyus, P. maximum and Brachiaria decumbens for ADF content. Data on the mineral concentration showed that C. plectostachyus in the dry and rainy seasons had a higher calcium content than those of other species. The overall means of the seven grasses for IVDMD, IVOMD and IVCPD were significantly higher (P < 0.05) in the rainy season than in the dry season. The in vitro gas production and ME concentration were numerically higher in the rainy season than in the dry season. In conclusion, the nutritive value of the observed grasses in North Sumatra was relatively higher during the rainy season compared with the dry season. Among the species of grass, P. purpureum and C. plectostachyus had a higher nutritive value in both the dry and rainy seasons.     

Production of transgenic cloned pigs expressing the far-red fluorescent protein monomeric Plum

Monomeric Plum (Plum), a far-red fluorescent protein with photostability and photopermeability, is potentially suitable for in vivo imaging and detection of fluorescence in body tissues. The aim of this study was to generate transgenic cloned pigs exhibiting systemic expression of Plum using somatic cell nuclear transfer (SCNT) technology. Nuclear donor cells for SCNT were obtained by introducing a Plum-expression vector driven by a combination of the cytomegalovirus early enhancer and chicken beta-actin promoter into porcine fetal fibroblasts (PFFs). The cleavage and blastocyst formation rates of reconstructed SCNT embryos were 81.0% (34/42) and 78.6% (33/42), respectively. At 36–37 days of gestation, three fetuses systemically expressing Plum were obtained from one recipient to which 103 SCNT embryos were transferred (3/103, 2.9%). For generation of offspring expressing Plum, rejuvenated PFFs were established from one cloned fetus and used as nuclear donor cells. Four cloned offspring and one stillborn cloned offspring were produced from one recipient to which 117 SCNT embryos were transferred (5/117, 4.3%). All offspring exhibited high levels of Plum fluorescence in blood cells, such as lymphocytes, monocytes and granulocytes. In addition, the skin, heart, kidney, pancreas, liver and spleen also exhibited Plum expression. These observations demonstrated that transfer of the Plum gene did not interfere with the development of porcine SCNT embryos and resulted in the successful generation of transgenic cloned pigs that systemically expressed Plum. This is the first report of the generation and characterization of transgenic cloned pigs expressing the far-red fluorescent protein Plum.     

ASAS-NANP SYMPOSIUM: prospects for interactive and dynamic graphics in the era of data-rich animal science

Statistical graphics, and data visualization, play an essential but under-utilized, role for data analysis in animal science, and also to visually illustrate the concepts, ideas, or outputs of research and in curricula. The recent rise in web technologies and ubiquitous availability of web browsers enables easier sharing of interactive and dynamic graphics. Interactivity and dynamic feedback enhance human–computer interaction and data exploration. Web applications such as decision support systems coupled with multimedia tools synergize with interactive and dynamic graphics. However, the importance of graphics for effectively communicating data, understanding data uncertainty, and the state of the field of interactive and dynamic graphics is underappreciated in animal science. To address this gap, we describe the current state of graphical methodology and technology that might be more broadly adopted. This includes an explanation of a conceptual framework for effective graphics construction. The ideas and technology are illustrated using publicly available animal datasets. We foresee that many new types of big and complex data being generated in precision livestock farming create exciting opportunities for applying interactive and dynamic graphics to improve data analysis and make data-supported decisions.     

Effects of concentrate supplementation on blood mineral concentration of growing upgraded Philippine goats

A total of 32 4‐month‐old‐upgraded Philippine goats with mean bodyweight of 13.5 kg were used to study the effects of concentrate supplementation on mineral status of growing goats. All animals received a basal diet comprised of different forage species. Twenty of the animals were supplemented with concentrate at a rate of 150–200 g dry matter/day while the remaining 12 animals were not supplemented. Blood sampling and bodyweight measurement were done every 2 weeks during the 5 months of experiment. Forage, concentrate and blood sulfur, calcium, phosphorus, magnesium, copper, zinc and selenium concentration were analyzed after wet digestion. Forage mineral contents, except for zinc and selenium, were sufficient for requirements of ruminants. Concentrate mineral contents were much higher than the requirements, but did not exceed the toxic levels. The concentrate supplementation resulted in high (P < 0.05) plasma phosphorus, copper, zinc, whole blood selenium concentrations and bodyweight of growing goats, although some supplemented animals showed plasma zinc concentration below the lower critical level. It was recommended that pellets, glass bolus or capsules that contain deficient minerals, such as zinc and selenium, should be used in the study area.     

Is Leucaena leaf useful as a selenium supplement for growing Philippine goats?

The effects of Leucaena leucocephala leaf (LL) supplementation on selenium (Se) status of growing Philippine goats were determined in the present study. A total of 12 3–4 month old male goats with mean bodyweight of 8.8 kg was used. All animals received a basal diet comprised of different forages. Six of the animals were supplemented with LL (22.9% CP) while the remaining six animals were not supplemented (control). The rate of LL to the total forage diet offered to the LL‐supplemented animals was 25%. Bodyweight measurement, blood and forage sampling were carried out every 2 weeks during the 4 months of experiment. Selenium, sulfur, calcium, phosphorus, magnesium, copper and zinc concentrations were analyzed after wet digestion. Leucaena leaf seemed to have higher Se and calcium, but lower copper and zinc concentrations than was observed in grasses fed to the animals. Bodyweight of growing goats in LL‐supplemented group tended to be higher than that of control animals. Supplementation of LL resulted in high whole blood Se concentration of growing goats. In the last month of this experiment, whole blood in all supplemented animals contained higher Se concentration than the deficient level of 20 ng/mL, while Se concentration in the control group was slightly above the deficient level.     

An immunological investigation of human pituitary growth hormone

Growth hormone isolated from human pituitaries has been demonstrated to be a good antigen in the rabbit. With the rabbit antiserum to human somatotropin, it is possible to detect as little as 0.1 microg of the hormone by precipitin test. The antiserum was also capable of neutralizing the growth-promoting activity of human somatotropin.