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1.
Distribution of alpha‐neoendorphin,ACTH (18–39) and beta‐endorphin (1–27) in the alpaca brainstem 下载免费PDF全文
Manuel L. Sánchez Eliana de Souza Luis A. Aguilar Rafael Coveñas 《Anatomia, histologia, embryologia》2018,47(5):481-492
Using an immunocytochemical technique, we have studied in the alpaca brainstem the distribution of immunoreactive structures containing prodynorphin (alpha‐neoendorphin)‐ and pro‐opiomelanocortin (adrenocorticotrophin hormone (18–39) (ACTH), beta‐endorphin (1–27))‐derived peptides. No peptidergic‐immunoreactive cell body was observed. Immunoreactive fibres were widely distributed, although in most of the brainstem nuclei the density of the peptidergic fibres was low or very low. In general, the distribution of the immunoreactive fibres containing the peptides studied was very similar. A close anatomical relationship occurred among the fibres containing alpha‐neoendorphin, ACTH or beta‐endorphin (1–27), suggesting a functional interaction among the three peptides in many of the brainstem nuclei. The number of fibres belonging to the prodynorphin system was higher than that of the pro‐opiomelanocortin system. A moderate/low density of immunoreactive fibres was observed in 65.11% (for alpha‐neoendorphin (1–27)), 18.18% (for ACTH) and 13.95% (for beta‐endorphin) of the brainstem nuclei/tracts. In the alpaca brainstem, a high density of immunoreactive fibres was not observed. The neuroanatomical distribution of the immunoreactive fibres suggests that the peptides studied are involved in auditory, motor, gastric, feeding, vigilance, stress, respiratory and cardiovascular mechanisms, taste response, sleep‐waking cycle and the control of pain transmission. 相似文献
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Bonfim Fernando César Gonçalves Dodonov Pavel Cazetta Eliana 《Landscape Ecology》2021,36(9):2535-2547
Landscape Ecology - Anthropogenic land use and cover changes impact biodiversity worldwide. However, ecological groups are differently affected by landscape composition and configuration.... 相似文献
4.
Franco Mutinelli Marta Vascellari Eliana Schiavon 《Journal of veterinary diagnostic investigation》2005,17(3):288-290
The macroscopic and microscopic features of a retroperitoneal perirenal pseudocyst in a 12-month-old ram without impairment of renal function are described. In humans and animals, uriniferous pseudocysts may be of traumatic origin, resulting from rupture of kidney, renal pelvis, or ureter, or congenital. Lymphatic pseudocysts may develop secondary to inflammatory obstruction of the hilar lymphatics after perinephritis or renal transplantation. In this case, histologic characteristics of the pseudocyst wall were suggestive of development from the parietal peritoneal layer encapsulating the kidney. This is the first case of retroperitoneal perirenal pseudocyst in a sheep. 相似文献
5.
Tani H Shimizu R Sasai K Baba E 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(10):1049-1056
Circulating thyroglobulin autoantibody (TgAA) was analyzed using the Western immunoblot for determination of the dominant epitopes recognized by TgAA on tryptic peptides of canine thyroglobulin (cTg) in hypothyroid dogs. TgAA was measured in hypothyroid dogs, non-hypothyroid dogs with skin diseases and clinically normal dogs. Five of the 7 hypothyroid dogs, 1 of the 8 dogs with skin diseases and 1 of the 4 normal dogs were positive for TgAA. Four of the 5 TgAA-positive hypothyroid dogs were Golden Retrievers, and 3 of them showed high antibody titers. The sera of TgAA positive-dogs reacted to several peptides, and their patterns varied from sample to sample. Sera from 3 dogs with high titers of TgAA reacted broadly to high molecular weight peptides ranging from 45 to 90 kDa. These Western immunoblot patterns of the sera were disappeared after pretreatment with sufficient amount of intact cTg. All serum samples of both TgAA positive dogs and negative controls reacted to low molecular weight peptides ranging from 15 to 20 kDa. These immunoblot patterns of the sera were not disappeared even after pretreatment with sufficient amount of intact cTg. These findings show the possibility that the epitopes recognized by TgAA depend upon individual dogs with hypothyroidism and these autoantibodies recognize conformational epitopes on the cTg molecule. 相似文献
6.
Paranhos-Silva M Oliveira GG Reis EA de Menezes RM Fernandes O Sherlock I Gomes RB Pontes-de-Carvalho LC dos-Santos WL 《Veterinary parasitology》2003,114(2):97-111
In this study, we compare the development of infection and/or disease in Beagle dogs intradermally infected with Leishmania chagasi, in the presence or absence of Lutzomyia longipalpis saliva, with those of intravenously infected animals.Spleen samples of all the animals inoculated with parasites had positive polymerase chain reaction tests for Leishmania DNA. Positive spleen cultures for Leishmania were detected earlier (P < or = 0.018) and were more frequent (five out of the five animals) in intravenously infected animals than in the intradermally infected animals, in presence (two out of the six animals) or absence (three out of the five animals) of salivary gland lysate of L. longipalpis. Significant increase in serum antibodies against Leishmania was observed only in the intravenously infected group (P = 0.004). In addition, dogs with infection confirmed by isolation of amastigotes or detection of parasite DNA were, nevertheless, negative for anti-Leishmania antibodies up to 5 months or more after infection. Only animals of the intravenously infected group developed progressive decreases in hematocrit (Pearson r = -0.8076, P = -0.0026) and hemoglobin (Pearson r = -0.8403, P = 0.0012) during the infection period. No significant difference in the course of infection was observed between groups of intradermally infected animals. The data presented herein confirms that the intradermal inoculation of dogs with Leishmania produces an asymptomatic form of infection. It also fails to show an advantage in using L. longipalpis saliva as an infection-enhancing agent in experimental canine leishmaniasis. 相似文献
7.
An avian pathogenic Escherichia coli (APEC) strain designated SHS4, isolated from a chicken with clinical signs of swollen head syndrome (SHS), adhered to but did not invade Hep-2 and tracheal epithelial cells. The PCR amplified fimA, csgA and tsh gene sequences. It produced Ia, Ib, E1, E3, K, and B colicins, but not colicin V and aerobactin. It harboured two plasmids of 60 and 98MDa and was resistant to streptomycin and tetracycline. Conjugation with a nalidixic acid (Na) resistant K-12 recipient strain (MS101) showed that the 98MDa plasmid did not transfer, whereas transfer of the 60MDa plasmid resulted in concomitant transfer of adhesion to Hep-2 and tracheal epithelial cells, production of the colicins Ia, E1, E3, and K, and the tsh-related DNA sequence. Transposon (TnphoA) mutagenesis of strain TR4 gave rise to strain Mut23, which lost its adhesive capacities, but was still able to express the same colicins as did strain TR4. PCR was able to amplify the tsh-related DNA sequence in this strain and a molecular probe based on transposon TnphoA indicated that the transposon was inserted in the 60MDa plasmid. Based on these results, we suggest that the 60MDa plasmid have adhesion genes, which may be responsible for the initial colonization of the upper respiratory tract of chickens. 相似文献
8.
Kazumi KISHIDA Mitsuhiro SAKASE Kenta MINAMI Miyuki M. ARAI Reiko SYOJI Namiko KOHAMA Takayuki AKIYAMA Akio OKA Hiroshi HARAYAMA Moriyuki FUKUSHIMA 《The Journal of reproduction and development》2015,61(6):519-524
The purposes of this study were to examine the relationship between male artificial insemination (AI)
fertility and sperm acrosomal conditions assessed by new and conventional staining techniques and to identify
possible reproductive dysfunctions causing low conception rates in AI using frozen-thawed spermatozoa with
poor acrosomal conditions in Japanese Black bulls. We investigated individual differences among bulls in the
results concerning (1) acrosomal conditions of frozen-thawed spermatozoa as assessed by not merely peanut
agglutinin-lectin staining (a conventional staining technique) but also immunostaining of acrosomal
tyrosine-phosphorylated proteins (a new staining technique), (2) routine AI using frozen-thawed spermatozoa as
assessed by pregnancy diagnosis, (3) in vivo fertilization of frozen-thawed spermatozoa and
early development of fertilized eggs as assessed by superovulation/AI-embryo collection tests and (4)
in vitro fertilization of frozen-thawed spermatozoa with oocytes. The percentages of
frozen-thawed spermatozoa with normal acrosomal conditions assessed by the abovementioned staining techniques
were significantly correlated with the conception rates of routine AI, rates of transferable embryos in
superovulation/AI-embryo collection tests and in vitro fertilization rates. These results are
consistent with new suggestions that the distribution of acrosomal tyrosine-phosphorylated proteins as well as
the acrosomal morphology of frozen-thawed spermatozoa are AI fertility-associated markers that are valid for
the prediction of AI results and that low conception rates in AI using frozen-thawed spermatozoa with poor
acrosomal conditions result from reproductive dysfunctions in the processes between sperm insemination into
females and early embryo development, probably failed fertilization of frozen-thawed spermatozoa with
oocytes. 相似文献
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Azusa SOMEYA Ryoko FUKUSHIMA Michiko YOSHIDA Yasuyuki TANAHASHI Tangmunkhong PRAPEUK Reiko IIZUKA Hiroshi HIRAMI Atsushi MATSUDA Shunichi TAKAHASHI Goro KURITA Takashi KIMURA Misuzu SEO Masayuki FUNABA Yoshii NISHINO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(8):1157-1160