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排序方式: 共有88条查询结果,搜索用时 31 毫秒
1.
Introduction: We evaluated the totally implantable subcutaneous vascular access port (VAP) in 16 cancer patients undergoing intermittent chemotherapy for more than 30 months.
Methods: Ports were surgically placed (The CompanionPort, Norfolk Vet Products, Skokie, Illinois 60076) in the jugular vein of 12 dogs and 4 cats between 1/2002 and 7/2004. Body weight determined polyurethane catheter size (4, 5, 7 fr.). The polysulfone port, surrounded by titanium, was anchored to subcutaneous tissue in the dorsolateral neck and confirmed with C‐arm fluoroscopy. All blood samples were obtained via VAP. Nine anticancer agents, other medications, crystalloids/colloids, and whole blood were administered. Ports were flushed every 4–5 weeks with heparinized saline solution (100 IU/ml). Removed catheters were submitted for bacteriology.
Results: Seven of 16 animals are still alive. VAP were used for 1.5 to more than 30 months with 4–60 injections/port. Catheter tips were visualized from the left atrium distally into the caudal vena cava. Adverse events included post‐operative subcutaneous bruising and/or hematoma (4/16), difficult aspiration (4/16), catheter malposition (1/16), positional flushing (1/16), and occlusion requiring replacement (1/16). No thrombus formation or extravasation was evident. Bacterial colonization without signs of septicemia was observed in 3/4 catheters.
Conclusions: VAP are an effective way of achieving long‐term venous access in the dog and cat. Complications are typically minor and infrequent. 相似文献
Methods: Ports were surgically placed (The CompanionPort, Norfolk Vet Products, Skokie, Illinois 60076) in the jugular vein of 12 dogs and 4 cats between 1/2002 and 7/2004. Body weight determined polyurethane catheter size (4, 5, 7 fr.). The polysulfone port, surrounded by titanium, was anchored to subcutaneous tissue in the dorsolateral neck and confirmed with C‐arm fluoroscopy. All blood samples were obtained via VAP. Nine anticancer agents, other medications, crystalloids/colloids, and whole blood were administered. Ports were flushed every 4–5 weeks with heparinized saline solution (100 IU/ml). Removed catheters were submitted for bacteriology.
Results: Seven of 16 animals are still alive. VAP were used for 1.5 to more than 30 months with 4–60 injections/port. Catheter tips were visualized from the left atrium distally into the caudal vena cava. Adverse events included post‐operative subcutaneous bruising and/or hematoma (4/16), difficult aspiration (4/16), catheter malposition (1/16), positional flushing (1/16), and occlusion requiring replacement (1/16). No thrombus formation or extravasation was evident. Bacterial colonization without signs of septicemia was observed in 3/4 catheters.
Conclusions: VAP are an effective way of achieving long‐term venous access in the dog and cat. Complications are typically minor and infrequent. 相似文献
2.
Gutierrez M Forster FI McConnell SA Cassidy JP Pollock JM Bryson DG 《Veterinary immunology and immunopathology》1999,71(3-4):321-334
In order to develop procedures to label the main bovine leucocyte populations in paraffin embedded sections, the immunoreactivity of 25 monoclonal antibodies (mAbs) to different leucocyte antigens was assessed with formal dichromate (FD5) and 10% formalin fixation, a battery of antigen retrieval (AR) methods, and the biotin-tyramide amplification system. All the leucocyte populations investigated (CD2+, CD4+, CD8+, WC1+ T lymphocytes, B cells and macrophages) were strongly and specifically detectable under an appropriate combination of mAb, AR method and signal amplification system. CD4 and CD8 required the most stringent conditions and could only be demonstrated in FD5 fixed sections. For detection of CD2, WC1+ T lymphocytes, B cells and macrophages, all the mAbs produced immunoreactivity in FD5 or formalin fixed tissues. The need to check a range of different AR methods is stressed, as the method of choice varied for each individual mAb. The incorporation of the signal amplification system was necessary to observe a strong signal and the complete distribution of CD4, CD8 and B cells. Fixation by FD5 proved to be better than formalin for the preservation of surface antigens but it was inferior for the detection of markers which were found to show cytoplasmic immunoreactivity, such as the macrophage marker MAC387 or the B cell markers BAQ155 or IL-A59. 相似文献
3.
Kinetic properties and substrate specificity of the lysine transport system in porcine mammary gland were studied using mammary tissue explants from nine lactating sows. Sodium dependence of lysine uptake was determined by replacing sodium in the medium with choline. Kinetic parameters of lysine uptake were determined using lysine concentrations from 5 microM to 5.12 mM. Competition of lysine uptake by other amino acids was determined using the cationic amino acids, arginine and ornithine, and using other essential amino acids. Transport of lysine was time-dependent and was unaffected by replacing sodium with choline. Lysine uptake occurred by a transport mechanism with a Km of approximately 1.4 mM and a Vmax of 7.9 mmol x kg cell water(-1) x 30 min(-1). Lysine uptake was inhibited by arginine and ornithine and by high concentrations of L-alanine, L-methionine, L-leucine, cycloleucine, and D-lysine, but not by 2-(methylamino)-isobutyric acid. This transport mechanism is the primary system responsible for uptake of cationic amino acids in lactating sow mammary tissue. The relatively high Km, compared with physiological blood concentrations of lysine, indicates that the kinetic properties of the lysine transport system should not be limiting to milk protein synthesis. Transmembrane transport of lysine by lactating sow mammary tissue should be a direct function of plasma concentrations. However, interactions of other amino acids with the uptake system may affect lysine uptake. 相似文献
4.
5.
William S. Marshall Sharon E. Bryson David R. Idler 《Fish physiology and biochemistry》1989,7(1-6):331-336
The sperm duct epithelium of brook trout (Salvelinus fontinalis), mountedin vitro in Ussing-style epithelial chambers actively absorbs Na+ (measured as the short-circuit current, Isc) and secretes K+ (measured using86Rb+ as tracer). Dibutyryl-cyclic-adenosine monophosphate (db-cAMP) and 3-isobutyl-1-methylxanthine (IMX) produce a rapid, sustained
stimulation of both ion transport processes, but the hormone connected to the response is unknown. Purified sockeye salmon
CON A2 gonadotropin (GtH) produces a dose-dependent, rapid and sustained rise in Na+ uptake and K+ secretion. The time course, electrophysiological and transport characteristics are similar to those evoked by IMX. Carbohydrate-poor
(chum salmon CON A1) GtH is ineffective. Pretreatment of fish with 17α,20β-dihydroxy-4-pregnen-3-one (17α,20β-P) significantly
increases milt volume but is without effect on resting or stimulated (IMX + db-cAMP) levels of sperm duct ion transport. This
is the first indication of a direct, rapid action of GtH on ion transport by the vertebrate blood-testis barrier. The results
suggest direct involvement of GtH in control of later stages of sperm maturation. 相似文献
6.
William S. Marshall Sharon E. Bryson M. Michelle Sapp 《Fish physiology and biochemistry》1990,8(1):19-28
Brook trout erythrocytes that were washed with and suspended in Ringer's solution with reduced glutathione (1.0 mM) maintained
steady state cell volume for up to 24h, while those without the thiol-protective agent steadily shrank. Changes in cell volume
(measured as packed cell volume, PCV) were evoked by acidic media (Ringer's at pH 6.8), hypoosmotic solutions (or both) and
intracellular K+ and Cl− concentrations were monitored over 4h. Acid-swollen cells failed to volume regulate or release K+ but had significantly elevated intracellular Cl− Osmotically-swollen cells at pH 7.8 but not at pH 6.8 underwent regulatory volume decrease (RVD) and returned to initial
levels in 2h, accompanied by release of K+ and Cl− In contrast, osmotically-shrunken cells did not show regulatory volume increase. The regulatory volume decrease and concomitant
K+ release were dependent on Cl− implying a direct or indirect coupling of K+ to Cl− transport in volume regulation. RVD was partially blocked by 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS, 0.1
mM), an anion exchange blocker, but was unaffected by amiloride (1.0 mM) which blocks Na+/H+ exchange. Amiloride and DIDS prevented the swelling response to low pH but had no effect on control cells, suggesting involvement
of Na+/H+ and Cl−/HCO3
− exchanges in acid-induced cell swelling. Quinine (1.0 mM) a known blocker of K+ channels, exacerbated the osmotically-induced swelling but had little effect on the subsequent RVD and release of KCl. The
results suggest that low extracellular pH inhibits neutral C−-dependent K+ release and the resultant regulatory volume decrease in osmotically-swollen cells. 相似文献
7.
John E. Bryson 《Water, air, and soil pollution》1975,4(2):273-292
The legal aspects of the Federal Government with respect to solid waste and resource recovery are presented and discussed. 相似文献
8.
During an investigation of natural in utero infection of cattle by Leptospira interrogans strains, infection (almost entirely caused by serovar hardjo) was diagnosed in 57 per cent of 505 calves (472 aborted fetuses, 20 stillborn calves and 13 perinatal deaths) examined over a six-year period. The prevalence of leptospire-infected fetuses showed a seasonal increase in September, October and December and was significantly higher in fetuses aborted by dairy cows than in fetuses aborted by beef cows. The majority of infected fetuses were aborted from the sixth month of gestation onwards. Cows which aborted infected fetuses had not previously exhibited overt signs of agalactia. There was an association between leptospiral infection and retention of fetal membranes. 相似文献
9.
Cantrell CL Klun JA Bryson CT Kobaisy M Duke SO 《Journal of agricultural and food chemistry》2005,53(15):5948-5953
Essential oil extracts from Callicarpa americana and Callicarpa japonica were investigated. Bioassay-guided fractionation of C. americana extracts using the yellow fever mosquito, Aedes aegypti, led to the isolation of alpha-humulene, humulene epoxide II, and intermedeol and a newly isolated terpenoid (callicarpenal). Similar work involving C. japonica resulted in the isolation of an additional compound, spathulenol, as well as the four compounds isolated from C. americana. Structure elucidation was performed on all isolated compounds using a combination of gas chromatography-mass spectrometry-electron ionization, high-resolution liquid chromatography-MS-electrospray ionization, and one- and two-dimensional NMR experiments. Heretofore, 13,14,15,16-tetranorclerodane, callicarpenal, has never been identified from natural sources. Complete (1)H and (13)C NMR assignment data are provided for this compound. In bite deterrent studies, spathulenol, intermedeol, and callicarpenal showed significant repellent activity against A. aegypti and Anopheles stephensi. 相似文献
10.