A comparison of the effect of short-acting and long-acting cloxacillin-based dry-cow therapy on somatic cell counts after calving in cows also given internal teat sealants

AIM: To compare, in cows treated with an internal teat sealant, the effect of short-acting and long-acting cloxacillin-based dry-cow therapy on somatic cell counts (SCC) after calving.

METHODS: Cows from a spring-calving, pasture-based dairy farm in the Manawatu-Whanganui region of New Zealand were randomly allocated to receive either a short-acting cloxacillin and ampicillin dry-cow therapy and internal teat sealant (n=291) or a long-acting cloxacillin and ampicillin dry-cow therapy and internal teat sealant (n=288) at the end of lactation. Cows were managed on-farm with routine husbandry procedures through the dry period and following calving. A multivariable logistic regression model was used to determine the association between length of action of dry-cow therapy and the proportion of cows with a SCC >150,000?cells/mL at the first herd test after calving.

RESULTS: Age of cow, mean SCC for the preceding season and interval from calving to the first post-calving herd test were all associated with the proportion of cows with an individual SCC >150,000?cells/mL at the first herd test (p<0.001) Treatment with the short-acting dry-cow therapy was not associated with decreased odds of cows having a SCC >150,000?cells/mL at the first herd test compared with treatment with long-acting dry-cow therapy (OR=0.724; 95% CI=0.40–1.30).

CONCLUSIONS AND CLINICAL RELEVANCE: In this herd, which routinely used internal teat sealants, the use of short-acting cloxacillin-based dry-cow therapy did not result in an increased proportion of cows with elevated SSC post-calving. This was a single farm, single year study but indicates that in this herd, changing from a long-acting to a short-acting antimicrobial may have no impact on the prevalence of subclinical mastitis.     

Optimization of Factors Affecting <Emphasis Type="Italic">In vitro</Emphasis> Establishment, <Emphasis Type="Italic">Ex vitro</Emphasis> Rooting and Hardening for Commercial Scale Multiplication of Silk Banana (<Emphasis Type="Italic">Musa</Emphasis> AAB)

Factors concerning aseptic culture establishment and hardening were studied in detail in choicest Silk Banana. Effect of size of sucker (small, medium and large), carbon concentration (10, 20 and 30 g/l), season of initiation (wet and dry) and pre-treatments such as segmentation and incision to the explants were studied during initiation. Further, hardening related factors such as substrates used for primary and secondary hardening, nursery nutrition (source and frequency of application) and pre-treatments for ex vitro rooting were also studied. Results revealed that small suckers were most suited for initiations with the least contamination, maximum establishment and higher percentage of greening. Lower concentration of sucrose, though delayed greening, resulted in 100?% establishment of explants. Initiations performed during the drier period were completely free from the fungal contamination and showed less bacterial contamination than those performed during the rainy season. Segmentation of explant into four parts during first subculture supported maximum shoot proliferation by overcoming apical dominance. Coir pith was observed to be the most congenial substrate during primary hardening, whereas coir pith alone or sand: red earth: coir pith (1:1:1) supported superior performance of plantlets during secondary hardening. Single application of mono ammonium phosphate improved growth of plantlets during secondary hardening. Pre-treatment with mono ammonium phosphate gave the best response in terms of rooting and hardening of un-rooted micro-shoots. Thus, the discussed methodology could help the industries to take up commercial scale propagation of Silk Banana.     

Cumulative Effect of Organic and Inorganic Sources of Nutrients on Yield,Nutrients Uptake and Economics by Rice–Wheat Cropping System in Indo-Gangetic Plains of India

ABSTRACT

Field experiments were conducted for 2 years in sandy loam soil, to study the direct effect of organic manures i.e. sewage sludge (SS), vermicompost (VC) and sesbania (SB) and chemical fertilizers on rice (Oryza sativa) and their residual effect on wheat (Triticum aestivum) grown in sequence in winter (Rabi) and summer (Kharif) season during 2015–2016 to 2016–2017 at Varanasi, Uttar Pradesh. Residual effect of organic sources of nutrients as SS, VC and SB were monitored up to fourth crop (II wheat) in sequence applied in conjunction with 75% recommended dose of fertilize (RDF). Among the nutrient sources, the maximum grain yield in I rice (4.89 t ha^?1), II rice (4.95 t ha^?1), was recorded in treatment T₃ (100% RDF with S, Zn, B) whereas in I wheat (4.68 t ha^?1) and II wheat (4.59 t ha^?1), it was recorded in T₄ (customized fertilizer). The maximum straw yield during all four crops was recorded in T₃ (100% RDF with S, Zn, B) in rice and T₄ (customized fertilizer) in wheat crop, which showed 25, 32, 23 and 28% increase over 100% RDF (T₂). Application of 100% RDF along with S, Zn, B and customized fertilizer increased the total uptake of N, P, K, S and B and also in net returns and B:C ratio followed by organic treatments.     

Dimensionality-driven insulator-to-metal transition in the bechgaard salts

Optical experiments were conducted on a series of organic linear chain conductors with different values of the interchain single-electron transfer integral tb, which quantifies the degree of anisotropy. Electron-electron interactions together with Umklapp scattering resulted in a correlation gap and an insulating state for small tb. An insulator-to-metal transition was observed when tb exceeded a critical value, on the order of the correlation gap Egap. The absence of a plasma edge on the insulator side of the transition for polarization perpendicular to the chains suggests that the electrons are confined to the chains. The optical features of the metallic state, when contrasted with the magnetic properties, are suggestive of spin-charge separation.     

The alternative breeding approaches for improving yield gains and stress response in pigeonpea (Cajanus cajan)

Pigeonpea is an important food legume crop of semi‐arid tropical regions. Plateauing of pigeonpea yield has been worrying breeders for the past 6–7 decades. Serious breeding efforts made during this period resulted in various high‐yielding and disease resistant cultivars. However, the gains in pigeonpea productivity have been modest. The authors, while reviewing this situation, conclude that long generation turnover, complexity of biological traits, low selection response and overreliance upon pedigree breeding present the key bottlenecks for this situation. In this paper, some alternative breeding approaches and technologies are suggested for the genetic enhancement of yield stability and stress response of pigeonpea.     

Impact of the use of biofertilizers on cotton ( Gossypium hirsutum ) crop under irrigated agro-ecosystem

High nitrogen fixing, phosphate solubilizing, phytohormones producing isolates of Azotobacter, Azospirillum, Acetobacter and Pseudomonas were used as inoculants for cotton. Important cultures were selected on the basis of their effect on root/shoot length and chemotactic behaviour. Selected bioinoculants were earlier tested for their beneficial properties like nitrogen fixation (ARA), ammonia excretion, IAA production etc. These bio-inoculants were further tested for phosphate solubilization property. Various chosen strains were tested with Desi (HD 123) and American (H 1098) cotton under field conditions (as for wheat). Plant height and boll weight were determined at the time of harvesting whereas survival rate of inoculated bacteria was determined after 30, 80 and 130 days respectively. In the year 2000–01, on the basis of boll number and boll weight plant^?1 AVK 51 (36; 76.2?g plant^?1), HT 57 (27; 56.9?g plant^?1), AC18 (33; 61.5?g plant^?1), Ala 27 (36; 61.4?g plant^?1) and Pseudomonas (34; 71.3?g plant^?1) were identified as significant both for American and desi cotton varieties. Highest survival rate was observed with Mac 68 (33.4 × 10⁵) followed by HT54 (31.5 × 10⁵) after 30 days of sowing, which decreased after 80 days and remained constant up to 130 days. This trend was observed with all the cultures. Similar results were observed in 2001–02. 25?kg ha^?1 N saving was observed with A. chroococcum (AVK51) bioinoculant for cotton crop.     

Integrated assessment of diversification of rice-wheat cropping system in Indo-Gangetic plain

Diversification of rice-wheat cropping system may improve the productivity and sustainability of agricultural production in the Indo-Gangetic plane region (IGPR), but the choice of alternative sequences to be used requires integrated assessment of various crop sequences. A long-term field experiment was initiated during 2000–01 on sandy loam soil at Banaras Hindu University, Varanasi, India to assess 10 rice-based cropping sequences – rice-wheat, rice-chickpea, rice-wheat-green gram, rice-wheat-Sesbania (green manure), rice-mustard-green gram, rice-lentil-cowpea (fodder), rice-pea, rice-lentil + mustard (3:1)-cowpea (fodder), rice-maize (cob) + vegetable pea (1:1)-cowpea (fodder) and rice-potato-green gram in randomized block design with four replications. The data contained in this paper are for the year 2002–03 and 2003–04. Substitution of rice-wheat with 300% intensity cropping sequences particularly with rice-potato-green gram resulted in maximum production cost but gave the highest system productivity, net return, benefit:cost ratio, production efficiency, profitability and energy productivity. Inclusion of summer grain/fodder legume or Sesbania for green manuring improved the content of soil organic matter. The soil P status was positively affected in all the sequences and particularly those with summer crops. Decline in soil available K in all the sequences indicated inadequacy of present recommended rate of fertilizer K to all the component crops in different sequences.     

A Pragmatic Approach for Obtaining Lines Possessing Desirable Traits in Silk Banana

Silk Banana is amongst the most popular groups of bananas grown in different parts of the world. However, the commercial scale cultivation requires identification of superior genotypes with desirable attributes including growth, yield, quality and resistance to Panama wilt disease which is a major concern. Panama disease caused by the fungus Fusarium oxysporum f. sp. cubense has been wreaking havoc in banana plantations throughout the world. A large number of varieties fall prey to the pathogen and hence world’s banana production has been threatened to a considerable extent, Silk Banana being the most vulnerable. Long term use of chemicals has not only failed to provide protection against the disease but has also raised concern about the environmental health. Resistance breeding is thus only viable alternative; however, breeding in banana is hindered by a number of inherent problems. In this communication, a systematic approach was proposed taking a typical case of Silk Banana var. Nanjanagud Rasabale to preselect lines possessing Panama wilt resistance and other desired traits. The proposed methodology comprised of identification of spontaneous variants in growing regions of the variety and their subsequent screening using a multi-pronged strategy. A number of lines exhibiting superiority in terms of tolerance to panama wilt, precocity, plant stature, physicochemical and shelf life related attributes have been reported. Considering overall performance, six promising lines were identified. This methodology has wider applicability as it could be effectively exploited with suitable modifications for identifying lines with desired traits in other varieties of banana.     

Concurrent Ex vitro Rooting and Hardening in Ney Poovan Banana (Musa AB): Effect of Carbon Sources and their Concentrations

Mass multiplication of non-Cavendish group of bananas has been limited due to non availability of reliable multiplication protocol and higher cost of production. The process of root induction and hardening are considered to be the most capital and labour intensive activities in micropropagation of any species. Carbon sources being one of the prime components governing the ex vitro survival of the plants need to be studied to maximize the success rate. Present is an attempt to study the effect of kind and concentration of carbon source on concurrent ex vitro rooting and hardening (CEVRH) in Ney Poovan banana. The results revealed that kind and concentration of carbon source present in the nutrient pool at the time of multiplication had a significant effect on the ex vitro performance of the plantlets. Better ex vitro rooting and hardening performance was exhibited by the shoots obtained from glucose (2?%) supplemented medium. Though rooting percentage was reduced in this treatment, considering the superior performance of the plantlets, it could be recommended to the commercial units for large-scale propagation of Ney Poovan banana plants. The concept of simultaneous rooting and hardening could be a boon for the varieties showing poor multiplication rate resulting in their higher cost of production.