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Thirty-six mice were inoculated intranasally with Bordetella parapertussis organisms (isolated from sheep with chronic non-progressive pneumonia) to study their deposition and clearance in the lower respiratory tract. The deposition of the organism was greater in the trachea than in the lungs. At 48 hours after inoculation, almost 100% of organisms were cleared from the trachea but only 55% of organisms were cleared from the lungs. This result correlates well with the morphological changes seen previously in the pulmonary parenchyma and airways of mice and lambs experimentally infected with this organism. 相似文献
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Twelve veterinarians were asked to estimate the percentage of lung surface that appeared consolidated in eleven projected colour transparencies of pneumonic ovine lungs. The estimates were compared to the pneumonic area measured by image analysis. Although all observers had some experience in veterinary pathology there was considerable variation between individuals in the accuracy of their estimates. Large lesions were underestimated and small lesions overestimated by most observers. 相似文献
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Sheep from local farms with and without previous exposure to pigs were tested for their skin and airway responses to a commercial Ascaris suum antigen. There was an immediate reaction to intradermal injection of the antigen in 90% of 101 sheep. A bronchial provocation test by aerosol of the same antigen was undertaken on 43 of the sheep with a positive skin reaction. About 70% of sheep showed an immediate airway response to the antigen as an aerosol, reflected as a significant increase in airway resistance and/or decrease of dynamic lung compliance. The mean peak airway resistance and mean lowest dynamic lung compliance were 165% above and 61% below their baselines, respectively. No significant changes were recorded when the same animals were given an aerosol of phosphate buffered saline. Similarly, no correlation was found between the degree of skin reaction and the magnitude of bronchoconstriction (p>0.05). The sheep with previous exposure to pigs showed no significant differences in airway responses to antigen challenge, although they showed significantly greater skin reactions than those without exposure to pigs. These results indicate that the majority of Romney sheep in the Manawatu have a natural skin and airway sensitivity to A. suum antigen and may therefore be used as an animal model to study human airway hypersensitivity. The origin of this sensitivity has yet to be determined. 相似文献
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We undertook a mark–recapture study of three pool‐dwelling balitoroid loaches and a nonmigratory goby in a Hong Kong hillstream, which extended over a 35‐week period. The target species were Pseudogastromyzon myersi and Liniparhomaloptera disparis (Balitoridae), Schistura fasciolata (Nemacheilidae) and Rhinogobius duospilus (Gobiidae). Recapture rates of tagged individuals were high (58% overall), especially for the abundant R. duospilus (70% of 412 tagged) and P. myersi (57% of 762 tagged). At the end of the study, most recaptured individuals (78% of gobies, 62% of P. myersi, 42% of L. disparis and 67% of S. fasciolata) were within the pools where they had been released. Of those that had moved elsewhere, maximum displacements ranged from 46 m (R. duospilus) to 101 m (P. myersi), giving rise to a strongly leptokurtic distribution of movement distances. Mean displacements after 35 weeks ranged from only 2.9 m (R. duospilus) to 10.0 m (L. disparis). The sedentary behaviour of these species was more marked than most other small benthic fishes, although our study excluded the breeding period and wet season when high flows might have stimulated movement. Among the mobile P. myersi individuals, most travelled upstream, whereas mobile gobies tended to move downstream. Juveniles of P. myersi and R. duospilus, as well as large adult P. myersi, were especially sedentary, while mobile adult P. myersi that travelled further showed smaller growth increments. The highly sedentary habits of all four fishes suggest that they may exert persistent local top‐down control of benthic communities within pools in Hong Kong streams. 相似文献
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OBJECTIVE: To develop a method for continuous infusion of gentamicin into the tarsocrural joint of horses, to determine pharmacokinetics of gentamicin in synovial fluid of the tarsocrural joint during continuous infusion, and to evaluate effects of continuous infusion of gentamicin on characteristics of the synovial fluid. ANIMALS: 12 healthy adult horses. PROCEDURE: An infusion catheter consisting of flow control tubing connected to a balloon infuser was used. Gentamicin solution (100 mg/ml) was infused in the right tarsocrural joint and balanced electrolyte solution was infused in the left tarsocrural joint for 5 days. Synovial fluid and serum gentamicin concentrations were measured by use of a fluorescence polarization immunoassay. RESULTS: 17 of the 24 (71%) infusion catheters initially placed functioned without complications for the entire 5-day infusion period. Median gentamicin concentration in synovial fluid from treated joints during the 5-day infusion period ranged from 2875 to 982 microg/ml. Median serum gentamicin concentration during this period ranged from 2.31 to 2.59 microg/ml. Mean (+/- SD) elimination half-life and total clearance of gentamicin from the synovial fluid were 6.25+/-1.01 hours and 1.52+/-0.96 ml/min, respectively. CONCLUSIONS AND CLINICAL RELEVANCE: An infusion catheter can be used for continuous infusion of gentamicin into the tarsocrural joints of horses for up to 5 days. At a gentamicin dosage of 0.17+/-0.02 mg/kg/h, continuous intra-articular infusion results in synovial fluid gentamicin concentrations greater than 100 times the minimal inhibitory concentration reported for common equine pathogens. 相似文献
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