Impacts of the ancient Maya on soils and soil erosion in the central Maya Lowlands

Many studies across the central and southern Maya Lowlands of Belize, Guatemala, Honduras, and Mexico have produced records of land degradation, mostly sedimentation and soil erosion, during the ancient Maya period from before 1000 BC to the Maya Collapse of c. AD 900. This paper provides new data from two sites (Blue Creek and Cancuén), synthesizes more than a decade of the authors' research in Guatemala, Belize, and Mexico, and synthesizes other findings from this region. These research projects analyzed more than 100 excavations in upland and depression sites, cored lakes and wetland sediments, and studied sediments in the field and laboratory using radiocarbon dating, a battery of soil chemistry tests, stratigraphic analysis, magnetic susceptibility, elemental analyses, and artifact identification. Our objective was to date when sedimentation and soil erosion occurred, identify stable surfaces, and correlate them with the state of knowledge about past land use. These findings indicate three general epochs of accelerated soil erosion and identified two major paleosols. The three waves of soil erosion occurred in the Preclassic period (c. 1000 BC to AD 250), the Late Classic (AD 550 to 900), and in the last several decades. The major paleosol (‘Eklu'um’) in these sites is a well-developed Mollisol or Vertisol that started forming in the early Holocene and was buried in either the Preclassic or Classic periods (AD 250 to 900). At some sites the Eklu'um paleosol lies beneath sediments with a fainter paleosol, which in turn lies buried below Classic period and later sediments. This picture shows higher than expected soil erosion linked to the region's first pioneer farmers in the Preclassic and less than expected soil erosion in the Late Classic when population peaked and land use was the most intensive. In other regions like Cancuén, Guatemala, however, most soil erosion occurred during the Maya Late Classic (AD 550–830). Erosion here was intense but short-lived: depressions record 1–3 m of aggradation in two centuries. A third epoch of accelerated soil loss and aggradation arose with the rapid land use changes brought by new pioneers during the last several decades.     

Catalytic hydrolysis of vasoactive intestinal peptide by human autoantibody

Vasoactive intestinal peptide (VIP) labeled with 125I, [Tyr10-125I]VIP, can be hydrolyzed by immunoglobulin G (IgG) purified from a human subject, as judged by trichloroacetic acid precipitation and reversed-phase high-performance liquid chromatography (HPLC). The hydrolytic activity was precipitated by antibody to human IgG, it was bound by immobilized protein G and showed a molecular mass close to 150 kilodaltons by gel filtration chromatography, properties similar to those of authentic IgG. The Fab fragment, prepared from IgG by papain treatment, retained the VIP hydrolytic activity of the IgG. Peptide fragments produced by treatment of VIP with the antibody fraction were purified by reversed-phase HPLC and identified by fast atom bombardment-mass spectrometry and peptide sequencing. The scissile bond in VIP deduced from these experiments was Gln16-Met17. The antibody concentration (73.4 fmol per milligram of IgG) and the Kd (0.4 nM) were computed from analysis of VIP binding under conditions that did not result in peptide hydrolysis. Analysis of the antibody-mediated VIP hydrolysis at varying concentrations of substrate suggested conformity with Michaelis-Menton kinetics (Km). The values for Km (37.9 X 10(-9) M) and the turnover number kcat (15.6 min-1) suggested relatively tight VIP binding and a moderate catalytic efficiency of the antibody.     

Synthesis of Novel Thin-Film Materials by Pulsed Laser Deposition

Pulsed laser deposition (PLD) is a conceptually and experimentally simple yet highly versatile tool for thin-film and multilayer research. Its advantages for the film growth of oxides and other chemically complex materials include stoichiometric transfer, growth from an energetic beam, reactive deposition, and inherent simplicity for the growth of multilayered structures. With the use of PLD, artificially layered materials and metastable phases have been created and their properties varied by control of the layer thicknesses. In situ monitoring techniques have provided information about the role of energetic species in the formation of ultrahard phases and in the doping of semiconductors. Cluster-assembled nanocrystalline and composite films offer opportunities to control and produce new combinations of properties with PLD.