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1.
Soil N mineralization is affected by microbial biomass and respiration, which are limited by available C and N. To examine
the relationship between C and N for soil microbial dynamics and N dynamics, we conducted long-term laboratory incubation
(150 days) after C and N amendment and measured changes in C and N mineralization, microbial biomass C, and dissolved C and
N throughout the incubation period. The study soil was volcanic immature soil from the southern part of Japan, which contains
lower C and N compared with other Japanese forest soils. Despite this, the area is covered by well-developed natural and plantation
forests. Carbon amendment resulted in an increase in both microbial biomass and respiration, and net N mineralization decreased,
probably due to increasing microbial immobilization. In contrast, N amendment resulted in a decrease in microbial respiration
and an increase in net N mineralization, possibly due to decreased immobilization by microbes. Amendment of both C and N simultaneously
did not affect microbial biomass and respiration, although net N mineralization was slightly increased. The results suggested
that inhibitory effect on microbial respiration by N amendment should be reduced if carbon availability is higher. Thus, soil
available C may limit microbial biomass and respiration in this volcanic immature soil. Even in immature soil where C and
N substrate is low, soil C, such as plant root exudates and materials from above- and belowground dead organisms, might help
to maintain microbial activity and N mineralization in this study site. 相似文献
2.
Abstract. Cyprinid herpesvirus 1 (CHV) or Herpesvirus cyprini was virulent for carp, Cyprinus carpio L., fry following 1 h immersion in water at 20 °C. Cumulative mortality for carp fry was 86–97% in 2-week-old common carp, 20% in 4-week-old fancy carp, and 0% in both 8-week-old common and fancy carp. The virus did not produce mortality in fry of crucian carp, grass carp or other cyprinids. It was also oncogenic in carp, inducing papillomas to the extent of 55% among both common and fancy carp fry. The neoplasms appeared 5–6 months after carp had been exposed to the virus by immersion and recurred at an incidence of 83% in carp 7·5 months post-desquamation of the tumour. The CHV was reisolated from all moribund fish and from all survivors. It also induced papillomas at an incidence of 13% in adult mirror carp and at 10% in adult fancy carp 5 months after intraperitoneal inoculation of 105 TCID50 ml-1 fish. The virus was rcisolated only from the ncoplastic tissue and not from internal organs. The neoplasms were normally located on fin, skin or mandible, at the intraperitoneal inoculation site. Specific fluorescence for CHV antigen was frequently detected in the gills, liver, kidneys and intestine of 2-week-old fry from 3 to 21 days following challenge with CHV. It was found in greater concentrations in experimentally induced papillomata on 2-week-old carp fry survivors examined 24 weeks after challenge than in naturally occurring neoplasms. 相似文献
3.
Comparative gear tows were conducted during the summers of 1987–1990 in the Gulf of Alaska. Efficiencies of four types of ichthyoplankton sampling gear were compared with respect to total catch and size distribution of larval fish. Fish were sampled using the bongo net (333 and 505 μm mesh), 1 m Tucker net, 3 m Tucker net and the Methot frame net. Results indicate that the bongo and the 1 m Tucker nets caught similar numbers and size distribution of fish. The larger 3 m Tucker and Method frame nets caught a larger size class of fish but a smaller total catch. Although there was a slightly significant diel difference in total catch detected in the smaller nets, no diel differences in size distribution of fish caught were detected for any of the nets. 相似文献
4.
Paul Dijkstra Ayaka Ishizu Stephen C. Hart Egbert Schwartz Bruce A. Hungate 《Soil biology & biochemistry》2006,38(11):3257-3266
Stable isotope analysis is a powerful tool in the study of soil organic matter formation. It is often observed that more decomposed soil organic matter is 13C, and especially 15N-enriched relative to fresh litter and recent organic matter. We investigated whether this shift in isotope composition relates to the isotope composition of the microbial biomass, an important source for soil organic matter. We developed a new approach to determine the natural abundance C and N isotope composition of the microbial biomass across a broad range of soil types, vegetation, and climates. We found consistently that the soil microbial biomass was 15N-enriched relative to the total (3.2 ‰) and extractable N pools (3.7 ‰), and 13C-enriched relative to the extractable C pool (2.5 ‰). The microbial biomass was also 13C-enriched relative to total C for soils that exhibited a C3-plant signature (1.6 ‰), but 13C-depleted for soils with a C4 signature (−1.1 ‰). The latter was probably associated with an increase of annual C3 forbs in C4 grasslands after an extreme drought. These findings are in agreement with the proposed contribution of microbial products to the stabilized soil organic matter and may help explain the shift in isotope composition during soil organic matter formation. 相似文献
5.
Seiichiro Yonemura Masayuki Yokozawa Gen Sakurai Ayaka W. Kishimoto-Mo Nayeon Lee Shohei Murayama Kentaro Ishijima Yasuhito Shirato Hiroshi Koizumi 《Journal of Forest Research》2013,18(1):49-59
At the Takayama deciduous broadleaved forest Asiaflux site in Japan, the ecosystem carbon dynamics have been studied for more than two decades. In 2005, we installed non-dispersive infrared CO2 sensors in the soil below the site’s flux tower to systematically study vertical soil–air CO2 dynamics and explain the behavior of soil surface CO2 efflux. Soil–air CO2 concentrations measured from June 2005 through May 2006 showed sinusoidal variation, with maxima in July and minima in winter, similar to the soil CO2 effluxes measured simultaneously using open-flow chambers. Soil–air CO2 concentrations increased with soil depth from 5 to 50 cm: from 2,000 to 8,000 ppm in the summer and from 2,000 to 3,000 ppm in the winter under snow. Summer soil–air CO2 concentrations were positively correlated with soil moisture on daily and weekly scales, indicating that the Oi, Oe, and A horizons, where decomposition is accelerated by high-moisture conditions, contributed substantially to CO2 emissions. This result is consistent with the short residence time (about 2 h) of CO2 in the soil and larger emissions in shallow soil layers based on our diffusion model. We revealed for the first time that soil–air CO2 concentrations in winter were correlated with both snow depth and wind speed. CO2 transfer through the snow was hundreds of times the gas diffusion rates in the soil. Our estimate of the CO2 efflux during the snow-cover season was larger than previous estimates at TKY, and confirmed the important contribution of the snow-cover season to the site’s carbon dynamics. 相似文献
6.
J.C. RODRIGUEZ SOUZA C.A. STRÜSSMANN TAKASHIMA SEKINE & SHIMA 《Aquaculture Nutrition》1999,5(1):41-51
This study examined the capacity of Japanese spiny lobster Panulirus japonicus phyllosoma larvae to absorb nutrients directly from the surrounding water. For this purpose, 48-, 81-, 124-, 160-, and 264-day-old intermoult larvae were starved for 48 h, held for 30 min to 4 h in 0.05 g L–1 solutions or dispersions of cholesterol, peanut oil, saccharose and horseradish peroxidase in seawater, and subsequently examined by histological and ultrastructural methods. Lipids, carbohydrate, and protein were detected in either the lumen of the midgut gland tubules, the midgut gland cells, the haemolymph or in the cells of the epidermis of all larvae examined after each experimental period, with general improvement of the cell and tissue characteristics over unfed controls. The lipids were also found in the cuticle. Thus, the results provide evidence of nutrient absorption via the digestive tract, and possibly also via the integument. Structural adaptations that may be involved in the process of absorption were tentatively identified. Our observations suggest the possibility that particulate organic matter (POM) and dissolved organic matter (DOM) can be utilized by the P. japonicus phyllosoma larvae, perhaps as supplementary sources of nutrients to macroscopic diets. 相似文献
7.
Takishita Kiyotaka Kawai Rihoko Tsutsumi Ayaka Tanifuji Goro Otsubo Mayuko 《Fisheries Science》2020,86(4):633-643
Fisheries Science - Tidal flats are ecologically important as they support a large community of animals (e.g., crabs, mollusks, and polychaetes) and restore water quality. However, information... 相似文献
8.
Md. Shaheed Reza Nanami Mizusawa Ayaka Kumano Chiharu Oikawa Daisuke Ouchi Atsushi Kobiyama Yuichiro Yamada Yuri Ikeda Daisuke Ikeda Kazuho Ikeo Shigeru Sato Takehiko Ogata Toshiaki Kudo Mitsuru Jimbo Ko Yasumoto Kazutoshi Yoshitake Shugo Watabe 《Fisheries Science》2018,84(3):563-577
In an effort to determine genus- or species-level taxonomic profiles and diversity of bacterial consortia in the Tama River around urban Tokyo, next-generation sequencing technology targeting a 16S ribosomal RNA (rRNA) gene amplicon was employed. Metagenomic analysis performed by an Ion Personal Genome Machine after sequentially filtering samples through 5-, 0.8- and 0.2-μm filters yielded 1.48 Gb of 16S sequences (average 2.38 M reads/sample). The results indicated that half of the bacterial sequences belonged to Proteobacteria, followed by Bacteroidetes, Actinobacteria and Cyanobacteria. Flavobacterium (Bacteroidetes), possibly including a potential fish pathogen, was the most numerous genera in the Tama River metagenome, and accounted for?~?16% of assigned 16S reads, followed by Mycobacterium. Other dominant bacterial genera including Zoogloea, Sediminibacterium, Hyphomicrobium, Sphingopyxis, Thiothrix and Lysobacter, were thought to be associated with waste water and sludge. MiSeq metagenomic analysis revealed that environmental factors, particularly water temperature, influenced the bacterial composition throughout the year, with a strong negative correlation observed for Proteobacteria and a positive correlation for Bacteroidetes. In terms of bacterial genera, Flavobacterium was positively correlated with temperature, while Polaromonas, Pseudomonas and Bradyrhizobium were negatively correlated with this, suggesting dynamic change in the free-living bacterial population throughout the year and versatile adaptation strategies in relation to environmental factors. 相似文献
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10.
Mikuya IWANAGA Naoto IMAI Ayaka KAMIKAWA Kaho SHIMADA Masatoshi OKURA Daisuke TAKAMATSU Daijiro UEDA Mizuki NAKAYAMA Tomoyuki SHIBAHARA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):53
A 179-day-old calf, which was weak and stunted, showed neurological signs and was euthanized. Postmortem examination revealed extensive and severe cloudy area in the meninges, and pleural pneumonia. Gram-positive cocci were isolated from systemic organs. Biochemical and 16S rRNA gene sequence analyses identified the isolate as Streptococcus gallolyticus, and its subspecies was suggested to be gallolyticus (SGG). The isolate was classified as a novel sequence type (ST115) by the multilocus sequence typing scheme for SGG and showed susceptibility to penicillin, ampicillin, amoxicillin, florfenicol, sulfamethoxazole-trimethoprim, and chloramphenicol. Histopathologically, suppurative meningoencephalitis and perineuritis were detected. As SGG has been isolated solely from a cow with mastitis in Japan, this is the first SGG infection in a calf with suppurative meningoencephalitis and perineuritis in this country. 相似文献