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排序方式: 共有84条查询结果,搜索用时 46 毫秒
1.
Kathryn C Gamble Michael M Garner Laura Krause Thomas P Alvarado 《Journal of zoo and wildlife medicine》2004,35(3):361-369
Two adult female rock hyraxes (Procavia capensis) at the Dallas Zoo were confirmed with spontaneous diabetes mellitus from 1997-2000, whereas a third animal with a similar clinical presentation never became hyperglycemic. The pancreas in all three animals showed pancreatic islet fibrosis (PIF). Retrospective examination of medical records for rock hyraxes acquired by this collection or born into it from 1991-2002 identified eight more animals affected with PIE All affected animals, including three males and eight females, were 1-7 yr of age and presented either with vague clinical signs of soft feces and rough hair coat or were acutely moribund or dead. Clinical pathology data was available for seven of the animals before onset of overt clinical signs and revealed inappropriate hyperglycemia in six, as well as elevated serum concentrations of creatine phosphokinase, amylase, and lipase in all seven animals. Pedigree evaluation did not support a familial pattern for PIE Review of the histopathology findings from nine other zoologic collections with rock hyrax deaths during the study period identified six institutions with 12 additional cases genetically unrelated to the incident collection. Histopathology and viral serology did not support an infectious cause. Analysis of serum anti-islet and anti-insulin antibodies did not suggest autoimmune disease, and none of the animals had known exposure to toxic substances. Limited nutritional analyses did not support a nutritional basis for the condition, and the cause for PIF remains unknown. 相似文献
2.
Alvarado IR Villegas P El-Attrache J Jensen E Rosales G Perozo F Purvis LB 《Avian diseases》2007,51(1):27-32
An avian adenovirus (AAV) was isolated from liver samples of two 2-wk-old broiler-breeder flocks obtained from grandparents vaccinated at 10 and 17 wks of age with an autogenous inactivated vaccine containing the European AAV 8 (8565 strain) and 11 (1047 strain) serotypes (AAV8/11 vaccine). Affected broiler-breeders exhibited clinical signs and macroscopic and microscopic lesions associated with inclusion body hepatitis (IBH). The isolated adenovirus, identified as Stanford, was molecularly characterized as European serotype 9. The pathogenicity of the Stanford strain was confirmed after inoculation of specific-pathogen-free (SPF) chickens at 1-7 days of age, causing 100% and 20% mortality, respectively. The level of protection against IBH was evaluated in two broiler-breeder progenies from AAV 8/11-vaccinated grandparent flocks and a commercial broiler flock by challenge at 1 or 7 days of age with the AAV 8 and 11 serotypes and/or the Stanford strain. The broiler-breeder progenies and the commercial broiler flock exhibited protection against IBH after challenge. No significant differences in mean body weights were observed at 3 wk of age in any of the evaluated groups. We conclude that broiler-breeder progenies from 30- to 50-wk-old grandparents vaccinated with the AAV 8/11 vaccine were adequately protected against challenge with the AAV 8 and 11 serotypes and the Stanford strain. 相似文献
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R. M. Trethowan Alexei Morgunov Zhonghu He R. De Pauw J. Crossa M. Warburton Arman Baytasov Chunli Zhang M. Mergoum G. Alvarado 《Euphytica》2006,152(3):303-316
Spring sown bread wheat is grown at high latitudes in Europe, Asia and North America. However, it is not clear what the associations are among environments, particularly in Asia and North America, and whether or not cultivars developed in one region may adapt in another. A yield trial comprised of cultivars developed in northern Kazakhstan, western Siberia, the Canadian Prairies, northern USA, northeastern China and broadly adapted genotypes bred by CIMMYT in Mexico was planted in all the above mentioned environments in 2002–2004. In general, cultivars performed best within the regions they were developed. However, cultivars developed in northern Kazakhstan/western Siberia were the most broadly adapted at high latitudes; they were not significantly different for grain yield from the locally developed cultivars in both China and Canada. Stronger photoperiod response, greater plant height and larger seed weight appeared to be key adaptive features of these materials. At lower latitudes, the Kazakh/Siberian cultivars were significantly lower yielding than all other materials. When low latitude Mexican sites were removed from the analysis, the Chinese locations tended to associate, whereas most Canadian and Kazak/Siberian locations were negatively associated with those from China.
SSR analysis of the cultivars from each region split the materials into two general groups, one based on North American cultivars and one comprised of Kazakh/Siberian and Chinese cultivars. Lines developed in Mexico were spread across these two groupings. Evidence suggests that considerable scope exists to improve bread wheat adaptation at high latitudes globally through intercrossing materials originating from Asia and North America. 相似文献
6.
Aida Z. Kebede Baldev S. Dhillon Wolfgang Schipprack Jose L. Araus Marianne Bänziger Kassa Semagn Gregorio Alvarado Albrecht E. Melchinger 《Euphytica》2011,180(2):219-226
For in vivo production of doubled haploid (DH) lines in maize, the rate of haploid induction is of crucial importance. Maternal
haploid induction depends primarily on the inducer used as a pollinator. However, the source germplasm used as a maternal
parent and the environmental conditions for induction may also influence haploid induction and these aspects have not been
examined in tropical maize so far. The objectives of our study were to (i) monitor the variation for haploid induction rate
(HIR) among diverse source germplasm in tropical maize, (ii) determine the relative importance of general (GCA) and specific
(SCA) combining abilities for HIR, and (iii) investigate the influence of summer and winter seasons and genotype × season
interactions on this trait. Ten inbreds were mated in a half diallel design. The resulting 45 F1 single crosses were pollinated with the haploid inducer hybrid RWS × UH400 during the summer 2008 and winter 2009 seasons
in a lowland tropical environment in Mexico. HIR of the single crosses averaged over seasons ranged from 2.90 to 9.66% with
an overall mean of 6.74%. Mean HIR was significantly (P < 0.01) higher during the winter (7.37%) than summer season (6.11%). Significant (P < 0.01) variation was observed due to GCA effects of parental inbreds of single crosses but not for SCA, GCA × season and
SCA × season interactions. Our study underpins that a higher HIR in tropical maize can be obtained by selecting appropriate
source germplasm and undertaking pollination under favorable environmental conditions. 相似文献
7.
After amputation, freshwater planarians properly regenerate a head or tail from the resulting anterior or posterior wound. The mechanisms that differentiate anterior from posterior and direct the replacement of the appropriate missing body parts are unknown. We found that in the planarian Schmidtea mediterranea, RNA interference (RNAi) of beta-catenin or dishevelled causes the inappropriate regeneration of a head instead of a tail at posterior amputations. Conversely, RNAi of the beta-catenin antagonist adenomatous polyposis coli results in the regeneration of a tail at anterior wounds. In addition, the silencing of beta-catenin is sufficient to transform the tail of uncut adult animals into a head. We suggest that beta-catenin functions as a molecular switch to specify and maintain anteroposterior identity during regeneration and homeostasis in planarians. 相似文献
8.
Egelhoff WF Stiles MD Pappas DP Pierce DT Byers JM Johnson MB Jonker BT Alvarado SF Gregg JF Bland JA Buhrman RA 《Science (New York, N.Y.)》2002,296(5571):1195; discussion 1195
9.
Reddien PW Oviedo NJ Jennings JR Jenkin JC Sánchez Alvarado A 《Science (New York, N.Y.)》2005,310(5752):1327-1330
We have identified two genes, smedwi-1 and smedwi-2, expressed in the dividing adult stem cells (neoblasts) of the planarian Schmidtea mediterranea. Both genes encode proteins that belong to the Argonaute/PIWI protein family and that share highest homology with those proteins defined by Drosophila PIWI. RNA interference (RNAi) of smedwi-2 blocks regeneration, even though neoblasts are present, irradiation-sensitive, and capable of proliferating in response to wounding; smedwi-2(RNAi) neoblast progeny migrate to sites of cell turnover but, unlike normal cells, fail at replacing aged tissue. We suggest that SMEDWI-2 functions within dividing neoblasts to support the generation of cells that promote regeneration and homeostasis. 相似文献
10.
PR WIDDERS KJ COATES S. WARNER JC BEATTIE IR MORGAN MW HICKEY† 《Australian veterinary journal》1995,72(6):208-211
SUMMARY The microbiological quality of carcases, meat and environmental surfaces was evaluated in commercial boning rooms processing beef and lamb. There was considerable variation in the level of microbial contamination on both carcases and meat, with counts ranging from less than 20 to 108/cm2 on carcases and to 2 times 107/cm2 on meat. The level of microbial contamination on meat was influenced by the level of carcase contamination at boning and by the boning process itself. Carcase contamination was the major determinant of microbiological quality, as more than 70% of carcases had microbial counts greater than 103/cm2. Cutting boards were a major source for microbial dissemination during boning, particularly when carcase counts were less than 103/cm2. If carcases were heavily contaminated, the contamination of processing surfaces was irrelevant in determining microbial loads on meat. Where carcase contamination was at low to moderate levels, the contribution of the boning process to the contamination on meat assumed increased significance. Under these conditions, improved sanitation of cutting surfaces in the boning room resulted in a significant reduction in microbial contamination on the surface of meat. These results can form the basis for ensuring that improvements made in carcase management before boning, to improve microbiological quality, will be preserved through attention to cutting board hygiene during boning. 相似文献