建兰花叶病毒TGBp1自身互作研究

Cymbidium mosaic virus (CymMV) is one of the most important viruses that cause orchids great losses. Triple gene block protein 1 (TGBp1) is a multifunctional protein of CymMV. Homologous interaction of CymMV TGBp1 is essential for its biological functions, but little is known about the site of homologous interaction of TGBp1. In this study, three truncated mutants of TGBp1-1 (motif I, Ia), TGBp1-2 (motif II, III, IV) and TGBp1-3 (motif V, VI) were constructed based on its motifs respectively. Yeast two-hybrid (Y2H), bimolecular fluorescence complementation (BiFC) and GST pull-down were used to screen the site of homologous interaction among three truncated mutants of TGBp1 and TGBp1. The results showed that TGBp1-2 (motif II, III, IV) was the main site of homologous interaction of TGBp1. Our results provide a foundation for further research on the movement and replication of CymMV.     

侧流免疫层析技术在兽医诊断中的应用

侧流免疫层析使用有色标记物、荧光标记物或其他标记物等作为标记物，用于偶联抗原(抗体),用以检测抗体(抗原)的一种免疫化学反应。传统的侧流免疫层析依赖于肉眼观察，灵敏度较低，只能提供定性或半定量结果。新一代侧流免疫层析技术采用不同类型标记物和应用外部设备，对待检物进行量化，来呈现待检物的含量，从而实现开发具有定量、多组分检测能力的免疫层析测试。论文总结了侧流免疫层析标记物、检测原理以及在兽医诊断中的应用，以期为今后侧流免疫层析技术在兽医临床诊断中的应用和改进提供理论参考。