Monitoring of blood vessels and tissues by a population of monocytes with patrolling behavior

The cellular immune response to tissue damage and infection requires the recruitment of blood leukocytes. This process is mediated through a classical multistep mechanism, which involves transient rolling on the endothelium and recognition of inflammation followed by extravasation. We have shown, by direct examination of blood monocyte functions in vivo, that a subset of monocytes patrols healthy tissues through long-range crawling on the resting endothelium. This patrolling behavior depended on the integrin LFA-1 and the chemokine receptor CX(3)CR1 and was required for rapid tissue invasion at the site of an infection by this "resident" monocyte population, which initiated an early immune response and differentiated into macrophages.     

Evaluation of feed utilization and growth performance of juvenile striped catfish Pangasianodon hypophthalmus fed diets with varying inclusion levels of corn gluten meal

A nutrition trial with striped catfish (Pangasianodon hypophthalmus) juveniles was undertaken to evaluate the effect of replacing dietary fishmeal (FM) protein with corn gluten meal (CGM). A diet with FM as the main protein source was used as the control diet (FM). Five experimental diets (approximately 320 g kg^?1 crude protein) were formulated to progressively replace 20% (CGM20), 40% (CGM40), 60% (CGM60), 80% (CGM80) and 100% (CGM100) of FM protein. Fifteen fish per tank (initial weight 11.2 ± 0.6 g) were randomly distributed into 18 80‐litre fibreglass tanks connected to a closed recirculation system (temperature 30.3 ± 1.0 °C). The diets were tested in triplicate for 12 weeks. The final weight and specific growth rate (SGR) of fish fed diets CGM20, CGM40 and CGM60 were not significantly different compared to fish fed the FM diet. Feed intake (FI) tended to decrease with increasing dietary CGM level. Striped catfish fed FM, CGM20 and CGM40 had significantly lower feed conversion ratio (FCR) compared with fish fed CGM80 and CGM100 (P < 0.05). The protein efficiency ratio (PER) of fish fed the CGM80 and CGM100 diets was significantly lower than those of all other treatments (P < 0.05). Total ammonia‐nitrogen (TAN) excretion increased with elevated dietary CGM inclusion. The viscerosomatic index (VSI) of fish fed the CGM80 and CGM100 diets were significantly higher (P < 0.05) than those of fish fed the other treatments. The crude lipid content in the final body composition of the striped catfish was elevated significantly with increasing dietary CGM levels. Fish fed the CGM80 and CGM100 diets displayed haematocrit levels significantly lower (P < 0.05) than those fed the other diets. The haemoglobin content in fish was significantly higher in fish fed CGM20 and lower at CGM100 compared to fish fed the FM diet. The results of the present trial indicated that the optimum level of FM protein replacement with CGM determined by quadratic regression analysis was 25.1% on the basis of maximum SGR.     

Spirulina as a natural carotenoid source on growth, pigmentation and reproductive performance of yellow tail cichlid Pseudotropheus acei

A feeding trial on yellow tail cichlid Pseudotropheus acei (Regan 1922) was undertaken to asses the effect of dietary Spirulina meal as a natural carotenoid source. Four experimental diets were formulated to progressively replace 0% (C), 2.5% (SP2.5), 5% (SP5) and 10% (SP10) of fish meal weight. Ten fish per tank (initial weight 3.75?±?0.02?g) were randomly distributed into twelve 80?l fiberglass tanks connected to a closed recirculation system (temperature 26.7?±?0.06°C). The diets were tested in triplicate for 12?weeks. The specific growth rate of fish fed all Spirulina diets were significantly higher compared to diet C. Feed intake (FI) tended to increase with dietary Spirulina level, and fish fed diet SP10 had significantly higher FI values compared to diet C. No significant differences in feed conversion ratio were observed among these groups. Although the protein efficiency ratio of fish fed diet C was lower than that of all Spirulina diets, no significant differences were observed among these groups (P?>?0.05). The total egg production and hatching rate (%) of eggs derived from all fish fed with Spirulina diets was significantly higher compared those from fish fed diet C. The yellow and blue coloration of yellow tail cichlid fed the diet containing Spirulina meal was enhanced and inclusion of dietary Spirulina meal was elevated carotenoids in skin. Results of the present study indicated that Spirulina meal has the potential to enhance the growth, reproductive performance and coloration on yellow tail cichlid.     

Behaviour of sulphur and nitrogen compounds measured at marine stations Lista and Säby in Scandinavia

Long distance transport of various air pollutants has been studied at two Scandinavian coastal sites. The measuring period has covered one year divided into 4 campaigns. Seasonal variations for sulphur and nitrogen compounds as well as anthropogenically emitted metals are reported. Concentrations of Pb and Zn as well as the ratio of V/Ni concentrations have been used to trace sulphuric episodes. Covariation of anthropogenic pollutants for the two sites is demonstrated.     

Distribution and population diversity of <Emphasis Type="Italic">Australian grapevine viroid</Emphasis> (AGVd) in Turkish autochthonous grapevine varieties

Several viroid species have been reported to infect grapevine in Turkey and there has been not much about their genetic diversity, except Australian grapevine viroid (AGVd, genus Apscaviroid, family Pospiviridae). The viroid was recently detected in Turkish vineyards with very little information about its variability. Therefore, the autochthonous grapevine varieties in two major grape-growing areas in Turkey, i.e. the Eastern Mediterranean and the Southeast Anatolia regions were tested for the prevalance and genetic diversity. The presence of AGVd in the autochthonous varieties was verified by RT-PCR. The incidence of AGVd infection was relatively low in both regions due to self-rooted nature of the plants in the areas. Comparative analysis of local AGVd isolates from these native varieties was performed with viroidal genome sequences from NCBI database. Consequently, AGVd populations in Turkey contained five major sequence variants consisting of three varieties. Four variants had minor sequence variations while one was remarkably diverse.     

Determination of Cadmium in Tap,Sea and Waste Water Samples by Vortex-Assisted Dispersive Liquid-Liquid-Solidified Floating Organic Drop Microextraction and Slotted Quartz Tube FAAS After Complexation with a Imidazole Based Ligand

This study presents a combination of dispersive liquid-liquid-solidified floating organic drop microextraction (DLLSFODM) and slotted quartz tube (SQT) with conventional flame atomic absorption spectrometry (FAAS) to improve the sensitivity for cadmium determination. A ligand namely 2-(4-methylphenyl)-1H-imidazo-[4,5-f]-[1,10]-phenanthroline which has not been used in trace analyte determination was used to form a cadmium complex. Stepwise optimization of parameters affecting complex formation (pH, ligand, and buffer solution) and extraction (extraction and dispersive solvents, salt effect and mixing) was done to maximize cadmium absorbance. The slotted quartz tube was fitted onto the flame burner and optimized to increase residence time of atoms in the flame. Instrumental parameters such as sample and fuel flow rate were also optimized to further enhance the absorbance signal for cadmium. Using optimal parameters and values, the limits of detection and quantification were determined to be 0.81 and 2.69 μg L^?1, respectively. Low percent relative standard deviations (<?6.0%) indicated good precision for both extraction and instrumental measurements. Recovery tests were used to determine the accuracy of the method and the recovery results obtained were between 88 and 113%.

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The Spatial Dimension of Trade‐ and FDI‐driven Productivity Growth in Chinese Provinces: A Global Cointegration Approach

This paper analyses the major determinants of long‐ and short‐run labour productivity evolution for Chinese provinces between 1978 and 2010. The role played by openness to trade and foreign direct investment (FDI) constitutes the main focus of this analysis. From a methodological perspective, our main contribution is the inclusion of spatial effects into a dynamic error correction modelling framework. The results show that, in addition to domestic factors such as investment intensity and infrastructure use, trade openness and inward FDI also exert a direct impact on labour productivity. Furthermore, the geographical environment has a strong indirect influence on productivity: The more a region is surrounded by high‐productive regions with good infrastructure and linkages to the world economy, the higher are its productivity level and growth rate. The magnitude of these impacts varies by spatial regime (coastal, interior provinces) and time period in focus. Especially in the recent past, trade and FDI activity appear to be increasingly important drivers of regional productivity evolution, both for coastal and interior regions. These findings have important policy implications: In order to fully exploit the benefits from such spillovers, coordinated industrial policies which foster regional complementarities and support the free movement of production factors across regional borders are crucial.     

Synthesis,Characterization, and Use of Carbon Microspheres for Removal of Different Dyes from Aqueous Environments

Water, Air, & Soil Pollution - Sustainable groundwater quality has become a major concern for the agro-based country like Bangladesh. Integrated approaches of various irrigation water quality...     

Dietary Spirulina (Arthrospira platensis,Gomont, 1892) improved the flesh quality and shelf life of rainbow trout (Oncorhynchus mykiss,Walbaum, 1792) fed fish meal or plant‐based diet

This study was carried out to determine the effects of fish meal or plant‐based diets with supplementation of dietary Spirulina (4%) on shelf life of rainbow trout, Oncorhynchus mykiss. Rainbow trout (initial weight: ~135 g) were fed four experimental diets (fish meal‐based FM, plant meal‐based‐PM, Spirulina included at 4% of fish meal‐based‐FM/S or plant meal‐based PM/S) for 12 weeks. After the feeding trial, trout were stored on ice at 4°C for 0, 5, 10, 15 and 20 days. The chemical, microbiological and sensory analyses of fish were subjected to each sampling days for flesh quality evaluation. TBA of supplemented dietary Spirulina was lower than that non‐supplemented dietary Spirulina. pH values of all groups decreased significantly between days 0 and 20 during the storage period (p < 0.05). Luminosity (L*) value of all groups increased with time in storage period. Fish fed Spirulina supplemented diets showed an increased red/green tonality (a*) (p < 0.05) compared with fish fed non‐Spirulina supplemented diets. In the end of the storage period, the total coliforms values of fish fed with PM/S diet lower than that of other all diets. In conclusion, dietary Spirulina could be allowed the shelf life of the rainbow trout to be extended.     

The effects of trout culture on diet and food availability of native freshwater fish populations

Cultivation of a species or population and distribution to various regions has raised the idea of negative impacts on native species. The effect of non‐native species on native species may be caused by competition for source, predation, hybridization, and parasite/disease transport. The present study aims to evaluate the possible effects of a trout farm established on the banks of Kocaba? Stream (Çanakkale, Turkey) on feeding characteristics of some freshwater fish (Phoxinus phoxinus, Barbus oligolepis, and Squalius cii). The samples were collected monthly over a year at two stations located before and after the trout farm and at a control station on the tributary not impacted by the effects of the farm. Fish and environmental samples were gathered monthly with an electroshocker and some fishing tools between August 2015 and July 2016. The fish diet and resource incorporation into biomass were evaluated by gut content and stable isotope analyses. Changes in diet preferences when the species are exposed to source stresses and some individuals fed on farm‐based feed contaminated from the farm in Kocaba? Stream. In this study, it was observed that native fish populations in aquaculture regions were influenced by diet preferences. Designing enclosed systems limiting feed and waste transit in aquaculture establishments may be suitable for sustainability of these species.