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1.
Shoshi Mizuta Yuko Nakanishi Masashi Shiraishi Yoshihiro Yokoyama Reiji Yoshinaka 《Fisheries Science》2007,73(6):1353-1361
ABSTRACT: To obtain fundamental information for the effective use of scallop Patinopecten yessoensis mantle, which is one of the underutilized marine resources. Some properties of collagen contained in the mantle were examined by chemical and histochemical techniques. Collagen content in the mantle varied annually, ranging from 0.98 to 1.72% of wet tissue, 7.7 to 12.6% of dry tissue and 13.5 to 26.5% of total protein, being relatively in high level of collagen content of invertebrate muscles. Collagen fiber was densely distributed in the inner connective tissue matrix of the mantle pallial, in contrast to the inner fold part which was rich in muscle fibers. The collagen contained in the crude collagen fraction (residue after alkali extraction), prepared from the mantle, was revealed to have considerably low solubility on hot-water extraction, constantly less than 20% of the total collagen at the temperatures in the range of 20–90°C. 相似文献
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ABSTRACT: A quantitatively major collagen was isolated from the pepsin-solubilized collagen preparation of the mantle by differential salt precipitation and phosphocellulose column chromatography, and its constituent α components (α1 and α2) were purified by phosphocellulose column chromatography. The subunits were demonstrated to be genetically distinct from each other by peptide mapping and amino acid analysis. The amino acid composition calculated from those of the α1 and α2 components in 2 : 1 ratio coincided well with that of the major collagen from the mantle. These results suggest that the major collagen in the mantle of the oyster may have a heterotrimer structure (α1)2 α2. 相似文献
3.
Studies of the uptake of dieldrin, lindane, and DDT by isolated rat hepatocytes in Krebs-Henseleit salt solution containing 2.34% bovine serum albumin have shown that the partition equilibrium of these hydrophobic organochlorine compounds is established very rapidly between the hepatocytes and the extracellular medium by reversible uptake and release processes. In the hepatocyte suspension system, these compounds are in dynamic partition equilibrium between the hepatocytes and the Krebs-Henseleit salt solution, and also between the bovine serum albumin molecules and the solution. The respective rate constants for the uptake and release processes were 0.83 and 2.52 × 10?2 sec?1 in a 2-ml suspension containing 106 cells. It has thus been demonstrated that the transport of hydrophobic organochlorine compounds between the hepatocytes and the extracellular medium is a much faster process than the metabolic transformation reaction in hepatocytes. 相似文献
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Selection logging is a principal management scheme in natural teak-bearing forests in Myanmar. Monitoring the spatial extent
and intensity of selection logging is important for sustainable forest management. This study applied the normalized difference
vegetation index (NDVI) image differencing method using two SPOT-5 pan-sharpened images (2.5 m spatial resolution) taken in
October 2007 and January 2009 to analyze canopy changes associated with damage from forest harvesting. According to the pixel-based
analysis, NDVI changes were larger in most logging road/log landing points whereas smaller NDVI changes were seen in most
unlogged points. NDVI changes in teak stump areas were related to distance from a logging road and the number of stumps within
the estimated crown area (a circular area with a 10 m radius from the center of each stump). A Fisher’s exact test showed that one of the main factors
causing the high NDVI change in teak stump areas was the effect of road construction. The distribution pattern of teak stumps
indicated that teak stumps with estimated crown areas that contained more than one stump had high mean change in NDVI. The spectral difference between before and after logging
revealed that logging roads had a greater effect on canopy changes than teak stumps. 相似文献
6.
We introduced mutations into prxA3a, a peroxidase gene of hybrid aspen, Populus kitakamiensis, to substitute the amino acid residues at the surface of the protein, and analyzed substrate specificities. PrxA3a and mutated enzymes heterogeneously gene expressed in Saccharomyces cerevisiae were purified by Ni affinity chromatography, hydrolysis of sugar chain (Endoglycosidase Hf) and gel filtration. The substrate specificities were altered by substituted amino acid residues. PrxA3a F77Y A165W acquired the substrate specificity to m-chlorophenol. PrxA3a F77Y and PrxA3a F77YA165W could polymerize sinapyl alcohol. In addition, PrxA3a A165W, F77Y, and F77YA165W improved cytochrome c oxidizing activity. These substituted amino acid residues should function as a catalytic site outside of the heme pocket. 相似文献
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New Forests - The genus Aquilaria (Thymelaeaceae) is widely used for the production of agarwood, and includes several valuable plantation species in Southeast Asia. To understand appropriate... 相似文献
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Kuniaki Nakata Ikki Yamamoto Yoshifumi Miyama Toru Nakamaru Reiji Masuda Masatomo Tagawa 《Fisheries Science》2017,83(6):1027-1035
In the aquaculture of the Japanese flounder Paralichthys olivaceus, hypermelanosis, a malpigmentation condition in which the scales of a significant area on the blind side express the characteristics of those on the ocular side, remains a major concern. Since introducing sand into the rearing tank effectively suppresses hypermelanosis, the inhibitory effects of various characteristics of the surface of the tank floor were investigated. Although an inhibitory effect was observed in both tanks with a sand-image floor and a sand-pasted floor, the strongest effect was found in tanks with a dimpled floor. In addition, covering the inner surface of the tank with net also inhibited hypermelanosis. Using a commercially available corrugated plate, the inhibition of hypermelanosis was confirmed with a noted increase in this effect when combined with light coloration. Juveniles tended to situate themselves in the valley portions of the corrugated plate, suggesting that floor contact with the blind side may contribute to hypermelanosis inhibition. Further observations on the floor contact area with various floor configurations suggested that hypermelanosis is locally suppressed in the areas with floor contact. Therefore, suppression by an undulated (both dimpled and corrugated) floor is likely due to an increase in floor contact area with the blind side. 相似文献
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Kazuo Satoh Tomohiro Tanaka Yuuichi Oguro Reiji Takahashi Tatsuaki Tokuyama 《Soil Science and Plant Nutrition》2013,59(5):777-781
Long-term preservation of ammonia-oxidizing bacteria had been achieved until now by using the silica gel-freezing method. Recently, the freeze-drying method for the preservation of microorganisms has been widely used, and has been applied to the preservation of ammonia-oxidizing bacteria. The 10% skim milk-l% pyruvate or glutamate ratio was optimum as a dispersion-medium, and the preservation temperature of the freeze-dried cells was effective at -80°C. This method of preservation was found to be considerably more effective than the silica gel-freezing method. 相似文献
10.
Software development for estimating the concentration of radioactive cesium in the skeletal muscles of cattle from blood samples 下载免费PDF全文
Tomokazu Fukuda Masahiro Hiji Yasushi Kino Yasuyuki Abe Hideaki Yamashiro Jin Kobayashi Yoshinaka Shimizu Atsushi Takahashi Toshihiko Suzuki Mirei Chiba Kazuya Inoue Yoshikazu Kuwahara Motoko Morimoto Masafumi Katayama Kenichiro Donai Hisashi Shinoda Tsutomu Sekine Manabu Fukumoto Emiko Isogai 《Animal Science Journal》2016,87(6):842-847
The 2011 earthquake severely damaged the Fukushima Daiichi Nuclear Power Plant (FNPP), resulting in the release of large quantities of radioactive material into the environment. The deposition of these radionuclides in rice straw as livestock feed led to the circulation of contaminated beef in the market. Based on the safety concern of the consumers, a reliable method for estimating concentrations of radioactive cesium in muscle tissue is needed. In this study, we analyzed the concentrations of radioactive cesium in the blood and skeletal muscle of 88 cattle, and detected a linear correlation between them. We then developed software that can be used to estimate radioactive cesium concentrations in muscle tissue from blood samples. Distribution of this software to the livestock production field would allow us to easily identify high‐risk cattle, which would be beyond the safety regulation, before shipping out to the market. This software is planned to be released as freeware. This software would contribute to food safety, and aid the recovery of the livestock industry from the damage creacted by the 2011 Tohoku earthquake and tsunami. 相似文献