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Mitsutoshi Nagase Kazuhiko Maeta Tadanori Aimi Katsuaki Suginaka Tsutomu Morinaga 《Fisheries Science》2009,75(3):811-816
Nucleotide sequence and polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis of the 3′-portion
of the mitochondrial 16S RNA gene (rDNA) coding sequence were used to differentiate between flying fishes and other fishes
that are frequently used in ago-noyaki production. In this study, we successfully distinguished between flying fishes and the other fishes by combining amplified
DNA fragments with universally designed primers and digesting the PCR products with AfaI and MfeI restriction endonucleases. PCR-RFLP of 15 ago-noyaki, 2 noyaki, and 8 other processed flying-fish products were analyzed to authenticate flying-fish content among processed seafood products.
After digestion of the PCR products with both enzymes, we found that all ago-noyaki and processed flying fish products contained either two or three DNA fragments of ~200, 300, and 530 bp, and noyaki samples (which do not contain flying fish) contained only one fragment of ~530 bp. Here, we present a new procedure to confirm
the content of flying-fish meal in ago-noyaki. 相似文献
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Akira Matsuda Yuki Shimizu Teppei Kanda Akihiro Ohnishi Noritaka Maeta Masahiro Miyabe Kaori Saeki Yoshiki Itoh 《Canadian journal of veterinary research》2021,85(3):236
Some immortalized lens epithelial cell lines have been established and are useful for molecular analysis. The establishment of additional cell lines must, however, enable a variety of in-vitro examinations. The objective of this study was to establish a new canine lens epithelial cell line by isolating CLC-1 cells from the lens tissue of a dog with cataracts. In CLC-1 cells, transforming growth factor beta (TGF-β) treatment significantly decreased gene expression of an epithelial marker and elevated that of mesenchymal markers; these characteristics are similar to those of a human lens epithelial cell line. Interestingly, CLC-1 cells exhibited lower expression of an epithelial marker and higher expression of mesenchymal markers than an anterior lens capsule. These results suggest that CLC-1 cells were derived from a cell population that was committed to epithelial-mesenchymal transition in cataract lens tissue. In conclusion, CLC-1 cells could be useful for analyzing molecular pathogenesis in canine cataracts. 相似文献
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Nagase Mitsutoshi Yi Ruirong Hidaka Fuminori Maeta Kazuhiko Aimi Tadanori Yamaguchi Takeshi Suginaka Katsuaki Morinaga Tsutomu 《Fisheries Science》2010,76(5):885-892
Real-time polymerase chain reaction (PCR) analysis of the 3′-portion of the mitochondrial 16S RNA gene (rDNA) coding sequence
was used to determine flying fish paste in ago-noyaki. We quantified the amount of flying fish paste in ago-noyaki samples using flying fish-specific primers (Tobi16SF3/Tobi16SR) and universal primers (Univ16SF2/Univ16SR2). Using real-time
PCR of standard ago-noyaki, a standard equation was obtained (y = 1.08x − 3.20; R
2 = 0.977). This equation was then used to estimate the relative flying fish paste contents of eight commercially available
ago-noyaki and two similar products. These results verified that the ago-noyaki products that had already been labeled with the E-mark deserved this status. 相似文献
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Kazuhiko Maeta Ayumi Koike Hiroyuki Mukaiyama Tomoya Ochi Takao Terashita Yutaka Kitamoto Tadanori Aimi 《Journal of Wood Science》2008,54(4):337-340
In order to develop a novel method of obtaining monokaryons for a mycorrhizal fungus, Lyophyllum shimeji, monokaryotization of dikaryotic stock culture via protoplast formation and regeneration was performed using 12 dikaryotic
stocks. From 6 dikaryotic stocks, a total of 120 monokaryons were isolated, and their mating compatibility was tested. Mating-compatible
monokaryons were successfully derived from a dikaryotic stock (NBRC 100325), and monokaryons of only 1 mating type relative
to the parental dikaryons were isolated from another 3 strains (MH01710, OK2L-1, and HY7L-1). We successfully prepared monokaryotic
stocks via protoplast monokaryotization, a technique that can be used to identify biological species of L. shimeji. This technique could be used for breeding various mycorrhizal mushrooms, including Tricholoma matsutake, for which the preparation of monospore cultures is extremely difficult.
Part of this study was presented at the 11th Annual Meeting of the Japanese Society of Mushroom Science and Biotechnology
(Asahikawa), September 2007 相似文献
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