青贮玉米促生菌的鉴定及生物学特性的研究

根据布坎南.R.E等著《伯杰细菌鉴定手册》（第八版）,对4株青贮玉米促进菌进行鉴定。结果表明,4株促生菌分属于短芽胞杆菌（Bacillus brevis),蜡质芽孢杆菌（Bacillus cereus）,放射形土壤杆菌（Agrobacteria radiobacter）和假单胞杆菌（Pseudomonas sp）。此餐,还对4株促生菌的生物学特性进行了比较观察。     

Long-term water balances in La Violada Irrigation District (Spain): II. Analysis of irrigation performance

The analysis of long-term irrigation performance series is a valuable tool to improve irrigation management and efficiency. This work focuses in the assessment of irrigation performance indices along years 1995-2008, and the cause-effect relationships with irrigation modernization works taking place in the 4000 ha surface-irrigated La Violada Irrigation District (VID). Irrigation management was poor, as shown by the low mean seasonal irrigation consumptive use coefficient (ICUC = 51%) and the high relative water deficit (RWD = 20%) and drainage fraction (DRF = 54%). April had the poorest irrigation performance because corn (with low water demand in this month) was irrigated to promote its emergence, whereas winter grains (with high water demands in this month) were not fully irrigated in water-scarce years. Corn, highly sensitive to water stress, was the crop with best irrigation performance because it was preferentially irrigated to minimize yield losses. The construction of a new elevated canal that decreased seepage and drainage fractions, the entrance in operation of six internal reservoirs that would increase irrigation scheduling flexibility, and the on-going transformation from surface to sprinkler irrigation systems are critical changes in VID that should lead to improved ICUC, lower RWD and lower DRF. The implications of these modernization works on the conservation of water quantity and quality within and outside VID is further discussed.     

Effect of diet composition on growth performance,hepatic metabolism and antioxidant activities in Siberian sturgeon (<Emphasis Type="Italic">Acipenser</Emphasis><Emphasis Type="Italic">baerii</Emphasis>, Brandt, 1869) submitted to starvation and refeeding

Many fish species undergo natural starvation periods. Adaptation to starvation is possible through the activation of behavioral, biochemical and physiological mechanisms. Knowledge of the effect of dietary nutrients on the intermediary metabolism during starvation and refeeding can be useful to improve fish health and optimize aquaculture production. To analyze the effect of dietary nutrients on liver metabolism of Siberian sturgeon (Acipenser baerii) submitted to starvation and refeeding, four isoenergetic diets differing in nutrient composition were designed: LP-St (38 % protein, 12 % lipid, 36 % carbohydrate), HP-St (44 % protein, 10 % lipid, 30 % carbohydrate), LP-L (38 % protein, 18 % lipid, 25 % carbohydrate) and HP-L (44 % protein, 16 % lipid, 22 % carbohydrate). Four groups of fish were fed 3 weeks to satiety with the corresponding diet, starved for 2 weeks and then refeed 5 weeks to satiety on the same diet. Starvation mobilized the hepatic lipid store to a greater extent than glycogen. Starvation increased superoxide dismutase activity irrespective of the diet, while low protein diets (LP-St and LP-L) increased catalase activity. The oxidative damage decreased after 5 weeks of refeeding. Refeeding the starved fish on the HP-St diet promoted the greatest growth performance. In addition to reporting for the first time the effect of diet composition on growth, liver composition and antioxidant activities in Siberian sturgeon submitted to starvation and refeeding, our findings suggest that refeeding on HP-St diet stimulated the use of dietary carbohydrates and allowed a protein sparing effect in Siberian sturgeon.     

Assessment of loose and adhered urban street sediments and trace metals: a study in the city of Poços de Caldas,Brazil

Purpose

This study aims to quantify and characterise sediments accumulated in the street gutters in an urban area of Poços de Caldas, Brazil. The main research questions are: What type of urban areas, e.g. those under construction, produce most sediments and what are the main characteristics of such sediments? What differences, e.g. granulometry, can be found in loose and adhered sediments? What trace metals can be found in the sediments?

Materials and methods

Fieldwork was carried out in a residential area of Poços de Caldas, Brazil. Ten samplings were conducted between May and August 2013 to collect sediments from road gutters. The collected sediments were then divided into ‘loose’ and ‘adhered’, depending on whether they were collected in a first, gentle, sweeping with soft bristled brush or in a subsequent sweeping with a stiff bristled brush. Granulometric curves were drawn for both types of sediments. Fine sediment analyses (≤63 μm) were performed on samples from the last five samplings. Two techniques were used to look for trace elements: energy dispersive X-ray fluorescence (EDXRF) and inductively coupled plasma-atomic emission spectrometry (ICP-AES).

Results and discussion

Larger amounts of sediments were collected after lower intensity rainfall events. Higher intensity events seemed to wash the sediments away. A correlation was found between areas under construction and sediment mass production. A characteristic range of granulometries (medium sand), found in our study is in accordance with studies by other authors. An important presence of heavy metals (Cr, Cd, Pb, Zn, Ni and Cu) was detected and characterised. As and Sn were also detected even though they are not often mentioned in the literature on urban soil pollutants.

Conclusions

Areas under construction were found to produce more sediments than other areas. The trace metals found in highest concentrations were Pb and As. The heavy metal concentration decreases after wet periods, showing that they are carried by runoff. It is expected that this study may serve as an input for establishing diffuse pollution control and mitigation strategies for the accumulation of pollutants in the urban environment.

     

Predicting soil salinity in response to different irrigation practices, soil types and rainfall scenarios

A model was developed to predict rootzone salinity under different irrigation practices on different soil types, with similar rainfall but different monthly distributions. A rootzone daily water and salt balance was performed using eight scenarios: two soil types (coarse textured vs. fine textured), two multi-year series of actual rainfall data and two irrigation practices (surface with fixed number of irrigations and ET-based sprinkler irrigation). All factors influenced the mean electrical conductivity (EC) of the rootzone in the growing season (EC_eS): (i) Surface irrigation led to lower EC_eS than sprinkler irrigation; (ii) Winter-concentrated rainfall caused lower EC_eS than rainfall distributed uniformly throughout the year; and (iii) Coarser-textured soil usually resulted in lower EC_eS than the finer textured. The EC_eS was related to the total precipitation of the hydrologic year and to the annual leaching fraction (LF) but surprisingly not to the seasonal LF. In most cases, the model predicted lower EC_eS than the FAO steady-state approach. Therefore, considering these site-specific features could lead to lower leaching requirements and the safe use of higher salinity water.     

Application of Organic Wastes in a Soil Polluted with Different Rates of Cr-Pb: Effects on Soil Biological Properties

An experiment was performed to determine the effects of adding municipal solid waste (MSW) and poultry manure (PM) to a soil polluted with chromium (Cr), lead (Pb), and Cr + Pb on the biological parameters of the soil. Soil was mixed with two solutions of Cr(NO₃)₃ and/or Pb(NO₃)₂ to give three concentrations (0, 100, and 250 mg Cr kg^?1 soil and 0, 100, and 250 mg Pb kg^?1 soil) and treated with MSW or PM. When the soil was contaminated with the metals without combining, the greatest adenosine triphosphate (ATP), urease, and phosphatase inhibition percentages occurred for 250 mg Pb kg^?1 soil. When the heavy metals were mixed, the inhibition of the biochemical parameters increased. The application of MSW and PM decreased the inhibition of the biochemical parameters and microbial population in the polluted soils. The inhibition percentage was greater for the soil amended with MSW than with PM, possibly due to the high humic acid concentration.     

<Emphasis Type="Italic">Cornus mas</Emphasis> (L.) Fruit as a Potential Source of Natural Health-Promoting Compounds: Physico-Chemical Characterisation of Bioactive Components

Interest in new sources of anti-inflammatory and antioxidant compounds has recently become a major research issue, with the cornelian cherry (Cornus mas L.) receiving particular attention for its significant amounts of phenolic compounds and vitamins, which exhibit a wide range of biological and pharmacological properties. This study was aimed at increasing knowledge regarding the cornelian cherry in Italy through the analysis of biologically active substances in the locally available genotype “Chieri”. Spectrophotometric methods were applied to evaluate antioxidant activity, total anthocyanin content and total polyphenolic content. Identification and quantification of the main phytochemical compounds (polyphenols, monoterpenes, organic acids and vitamin C) was performed via high performance liquid chromatography coupled to a diode array detector. C. mas extracts showed high levels of total soluble solids and low acidity. High amounts of phenolic secondary metabolites were observed, with particular reference to anthocyanins (134.71 mg_C3G/100 g_FW), which confer remarkable nutraceutical properties to the analysed samples. These results highlight the potential of C. mas fruits as a good source of natural antioxidants, suggesting their use as a functional food. Future studies should focus on identifying other specific phytochemical compounds and the genetic traits of local varieties in order to improve cornelian cherry cultivars for food and medicine production.     

Impact of starvation on digestive enzymes activities and plasma metabolites in Siberian sturgeon (Acipenser baerii,Brandt, 1869)

To increase the current knowledge about the relationship between nutritional status and the digestive capacity of Siberian sturgeon (Acipenser baerii), we addressed the effect of starvation‐refeeding and macronutrient composition on growth parameters and key digestive enzyme activities in A. baerii. Acipenser baerii juveniles were fed four different diets for 3 weeks, then starved for 2 weeks and allowed to refed for 5 weeks with the same diets. Another group of fish were fed 10 weeks with the corresponding diets. Among 10‐week fed fish, high‐protein diets promoted higher body weight values, while the lowest specific growth rate was observed in fish fed a low‐protein, medium‐carbohydrate, high‐lipid diet (p < .05). At the end of the experiment, in fish refed for 5 weeks following a feeding‐starvation cycle and in 10‐week fed animals, the higher levels of blood glucose, triglycerides and cholesterol were found in fish fed low‐protein diets (p < .05). In all treatments, 2 weeks of starvation decreased α‐amylase activity in the intestine (p < .05), while 4 days of refeeding increased lipase (p > .05) and α‐amylase activity in the intestine as well as pepsin in the stomach (p < .05). Our findings suggest that A. baerii maintains a high capacity to digest proteins and lipids after 2 weeks of starvation and that α‐amylase can be used as an indicator of the nutritional status in fish submitted to starvation‐refeeding cycles. Indeed, refeeding with high‐protein and CHO:L ratio diets after starvation could improve the growth rate of A. baerii in culture.     

Long-term water balances in La Violada irrigation district (Spain): I. Sequential assessment and minimization of closing errors

Long-term analysis of hydrologic series in irrigated areas allows identifying the main water balance components, minimizing closing errors and assessing changes in the hydrologic regime. The main water inputs [irrigation (I) and precipitation (P)] and outputs [outflow (Q) and potential (ET_c) crop evapotranspiration] in the 4000-ha La Violada irrigation district (VID) (Ebro River Basin, Spain) were measured or estimated from 1995 to 2008. A first-step, simplified water balance assuming steady state conditions (with error ? = I + P − Q − ET_c) showed that inputs were much lower than outputs in all years (average ? = −577 mm yr⁻¹ or −33% closing error). A second-step, improved water balance with the inclusion of other inputs (municipal waste waters, canal releases and lateral surface runoff) and the estimation of crop's actual evapotranspiration (ET_a) through a daily soil water balance reduced the average closing error to −13%. Since errors were always higher during the irrigated periods, when canals are full of water, a third-step, final water balance considered canal seepage (CS) as an additional input. The change in water storage in the system (ΔW) was also included in this step. CS and ΔW were estimated through a monthly soil–aquifer water balance, showing that CS was a significant component in VID. With the inclusion of CS and ΔW in the water balance equation, the 1998–2008 annual closing errors were within ±10% of total water outputs. This long-term, sequential water balance analysis in VID was an appropriate approach to accurately identify and quantify the most important water balance components while minimizing water balance closing errors.     

Irrigation modernization and water conservation in Spain: The case of Riegos del Alto Aragón

This study analyzes the effects of irrigation modernization on water conservation, using the Riegos del Alto Aragón (RAA) irrigation project (NE Spain, 123354 ha) as a case study. A conceptual approach, based on water accounting and water productivity, has been used. Traditional surface irrigation systems and modern sprinkler systems currently occupy 73% and 27% of the irrigated area, respectively. Virtually all the irrigated area is devoted to field crops. Nowadays, farmers are investing on irrigation modernization by switching from surface to sprinkler irrigation because of the lack of labour and the reduction of net incomes as a consequence of reduction in European subsidies, among other factors. At the RAA project, modern sprinkler systems present higher crop yields and more intense cropping patterns than traditional surface irrigation systems. Crop evapotranspiration and non-beneficial evapotranspiration (mainly wind drift and evaporation loses, WDEL) per unit area are higher in sprinkler irrigated than in surface irrigated areas. Our results indicate that irrigation modernization will increase water depletion and water use. Farmers will achieve higher productivity and better working conditions. Likewise, the expected decreases in RAA irrigation return flows will lead to improvements in the quality of the receiving water bodies. However, water productivity computed over water depletion will not vary with irrigation modernization due to the typical linear relationship between yield and evapotranspiration and to the effect of WDEL on the regional water balance. Future variations in crop and energy prices might change the conclusions on economic productivity.