Mutualism between euryhaline tilapia Sarotherodon melanotheron heudelotii and Chlorella sp.—Implications for nano-algal production in warmwater phytoplankton-based recirculating systems

The West-African euryhaline tilapia, Sarotherodon melanotheron heudelotii shift from visually feeding on zooplankton when juveniles to mostly filter feeding on phytoplankton when adults. When reared using an appropriate ration in intensive aquaculture systems, S. m. heudelotii also consume algal-based detritus, and contribute to sediment mineralization, clean up their environment, and ultimately stimulate and sustain algal growth. We analysed such practical advantages for phytoplankton-based recirculating systems, using S. m. heudelotii and Chlorella sp. as biological material originating from the prototype of such a system operated in Senegal. We performed a 24-h factorial design experiment in 36 tubs, cross-classifying three levels of S. m. heudelotii (fishless control, unfed fish, and fed fish) with four levels of Chlorella initial density.Chlorella overall mean density increased significantly from fishless, to unfed fish, and fed fish treatments, and with Chlorella initial density. S. m. heudelotii did not alter nitrogen nor phosphorus concentrations, only affected by algal initial densities. Most ammonia excreted by fish was probably uptaken by Chlorella. Bacteria-mediated diurnal nitrification was possibly an alternative ammonium loss mechanism at highest oxygen concentrations. Algae were not limited by nitrogen or phosphorus but most likely by low dissolved organic carbon availability. Chlorella differential responses with fed vs. unfed Sarotherodon suggest that CO₂ supplied by heterotrophic S. m. heudelotii respiration played a key role. Observed Chlorella growth rates were similar to the highest rates obtained in algal mass cultures, enriched with CO₂, nitrate and phosphate, under artificial lighting.Our results suggest the existence of a Sarotherodon-Chlorella mutualism in our systems, where S. m. heudelotii provide CO₂, the major limiting factor of Chlorella growth, whereas Chlorella oxygenate and detoxify the water media from ammonia, promoting S. m. heudelotii production. This mutualism could be used to optimize photosynthetic suspended-growth aquaculture systems, particularly in the Tropics where light is abundant and temperature is continuously high.     

Combined effect of ascorbic acid and gamma irradiation on microbial and sensorial characteristics of beef patties during refrigerated storage

The present study was undertaken to evaluate the effect of ascorbic acid concentrations (0.03 to 0.5%) and irradiation doses (0.5 to 4 kGy) on microbial growth, color coordinates (L, a, and b), and sensory characteristics (taste and odor) of beef patties during storage at 4 +/- 1 degrees C. Ascorbic acid was also compared to citric acid at a similar pH value in order to differentiate the effects of ascorbic acid from those of pH reduction. Results showed significant reduction (p< or = 0.05) of aerobic plate counts (APCs) and total coliforms, and a significant interaction (p< or = 0.05) between ascorbic acid and irradiation dose was observed. The irradiation treatment had detrimental effects on redness, yellowness, and hue angle values of meat. However, incorporation of ascorbic acid into the meat before irradiation resulted in significant (p< or = 0.05) stabilization of color parameters. The color improvement obtained with ascorbic acid was not related to the pH reduction. Also, no significant detrimental effect on taste or odor was found in irradiated samples containing ascorbic acid.     

Effect of native and allochthonous arbuscular mycorrhizal fungi on Casuarina equisetifolia growth and its root bacterial community

Exotic trees are often planted to recover degraded lands. Inoculation with mycorrhizal fungi can improve their survival. Plant growth is partly dependent on the strain used, but little attention has been paid to the selection of mycorrhizal fungi. The aim of this study was to determine whether the growth of Casuarina equisetifolia L. (Johnson) is affected by two different mycorrhizal inocula generated using fungal spores retrieved from an Australian site (allochthonous soil) and a Senegalese site (native soil) under C. equisetifolia trees. Comparative experiments were conducted with plants in a Senegalese soil, previously sterilized or not, and grown in a greenhouse. At harvest, parameters related to plant growth and mycorrhization were evaluated and soil bacterial communities were compared. Tree growth was significantly influenced by both types of inoculants. In unsterilized soil, plants inoculated with the native inoculant were taller than plants inoculated with the allochthonous inoculant and control plants. The frequency of mycorrhization with both inoculants was higher in unsterilized soil. The strongest effects of the mycorhizosphere on the soil microbiome were obtained with the allochthonous inoculum, and analysis of the taxonomic composition revealed mycorrhizal communities specific to each inoculum. These results suggest that the development of C. equisetifolia and its root bacterial community are dependent on the composition of the mycorrhizal inoculum. The functional consequences of this rhizosphere effect in terms of soil fertility should be further studied to better guide reforestation operations.     

Quality control of the vaccines and cold chain during the national vaccination campaigns]

During the two vaccination campaigns against rinderpest and contagious bovine pleuropneumonia (CBPP) in 1989 and 1990, 507 vaccine samples were collected for quality control at each step of the distribution chain from the Veterinary Pharmacy of Abidjan to the vaccinator on the field. Parallel to that, the quality of the cold chain was checked. A total of 463 titrations were performed. All the titration levels were above the OIE/FAO standards, i.e. 10(2.5) DICT50/ml for rinderpest Valence and 10(7) viable germs/ml for contagious bovine pleuropneumonia Valence.     

Ploughing frequency and compost application effects on soil infiltrability in a cotton–maize (Gossypium hirsutum–Zea mays L.) rotation system on a Ferric Luvisol and a Ferric Lixisol in Burkina Faso

One of the key issues to increase soil productivity in the Sahel is to ensure water infiltration and storage in the soil. We hypothesised that reducing tillage from annual to biennial ploughing and the use of organic matter, like compost, would better sustain soil hydraulic properties. The study had the objective to propose sustainable soil fertility management techniques in the cotton–maize cropping systems. The effects of reduced tillage (RT) and annual ploughing (AP) combined with compost application (Co) on soil infiltration parameters were assessed on two soil types. Topsoil mean saturated hydraulic conductivities (K_s) were between 9 and 48 mm h⁻¹ in the Luvisol, while in the Lixisol they were between 18 and 275 mm h⁻¹. In the two soil types compost additions with reduced tillage or with annual ploughing had the largest effect on K_s. Soil hydraulic behaviour was in reasonable agreement with soil pore size distribution (mean values varied from 19.5 to 237 μm) modified by tillage frequency and organo-mineral fertilization. Already the first 3 years of this study showed that use of organic matter, improved soil infiltration characteristics when annual ploughing was used. Also biennial ploughing showed promising results and may be a useful strategy for smallholders to manage these soils.     

Local perceptions and regional climate trends on the Central Plateau of Burkina Faso

Due to devastating droughts in the 1970s and 1980s, climatic and environmental change in the West African Sahel has attracted a great deal of scientific research. While many of these studies documented a long‐term trend of declining rainfall, analyses conducted in the last few years suggest that a ‘recovery’ is underway. Drawing on ethnographic interviews, focus groups, and participant observation in two Provinces of the Central Plateau of Burkina Faso, we elicited local perspectives on these rainfall trends from the people who are most directly affected, namely local farmers. Fieldwork revealed that farmers in the research sites perceive that both overall seasonal rainfall and the number of ‘big rains’ during the rainy season have decreased over the last 30 years. We then tested these perceptions against rainfall records from nearby meteorological stations and found them to be corroborated. This paper illustrates how farmers of the Central Plateau now view drought as ‘normal’, having incorporated drought‐mitigation adaptations into their agricultural systems. Our case study highlights the need for ground truthing scientific analyses and assessing livelihood implications at the local level. It also advocates for sustained institutional support for rural communities, to increase their ability to adapt to climate change. Copyright © 2008 John Wiley & Sons, Ltd.     

Sorghum-cowpea Intercropping: An Effective Technique Against Runoff and Soil Erosion in the Sahel (Saria,Burkina Faso)

In the Central Plateau of Burkina Faso, runoff on bare soil amounts to 40% of annual rainfall and soil losses reach 4 to 8 Mg ha-¹ a-¹, despite slopes of under 3%. Several studies have shown that mulching the soil surface can reduce runoff by over 60%. However, the scarcity of straw and the incompatibility of mulching with mechanical soil preparation have prevented the large-scale adoption of the technique. The study conducted in Saria village where annual rainfall is 800 mm,set out to evaluate the efficacy of sorghum-cowpea intercropping in reducing runoff and erosion. The study design comprised five plots set up so as to recover runoff and transported solids, on a Ferric lixisol with a slope of 0.7%. Three years results showed that sorghum-cowpea intercropping reduces runoff by 20-30% compared to a sorghum monoculture and by 45-55% compared to a cowpea monoculture. Soil loss is also reduced with intercropping by at least a half compared to sorghumand cowpea monoculture. Moreover, it transpired that sorghum-cowpea intercropping is also beneficial in agricultural production terms, since the grain yield of the intercropped plots was double that obtained with sorghumor cowpea monocultures. The better crop production can be an asset for the widespread use of this technique in the country. An evaluation of the advantages of the legume in terms of nitrogen supplies to the cereal (N 2 symbiotic fixation) and of added soil OM will be useful in improving the cost-effectiveness of such a technique.     

Carbon sequestration from common property resources: Lessons from community-based sustainable pasture management in north-central Mali

Carbon sequestration in soils has been presented as a potential mechanism to enhance productivity in semi-arid lands in Africa while contributing to the mitigation of greenhouse emissions. Most of the literature, however, focuses on assessing the capacity of existing technology to sequester carbon in soils. There is much less discussion in the literature on the social and institutional elements that need to be in place to realize the potential benefits of carbon sequestration. This paper contributes insights in this direction by analyzing a case of community-based pasture management in north-central Mali. The case study challenges common assumptions in carbon sequestration efforts, namely that land resources are devoted to a single use by resident users; have distinct boundaries and fall within identifiable territorial and administrative jurisdictions, and are subject to widely recognized claims and free of conflict. We suggest that this is not always the case. Findings indicate that carbon sequestration projects centered on rangelands need to allow for flexibility in livestock movements and resource availability and to account for the diverging interest of multiple stakeholders, including different types of pastoralists and farmers. We conclude that social capital formation and conflict management are key elements of a carbon sequestration strategy in supports of sustainable and equitable development in the Sahelian region.     

Mechanical and barrier properties of cross-linked soy and whey protein based films

Sterilized biofilms based on soy protein isolate (SPI, S system) and a 1:1 mixture of SPI and whey protein isolate (WPI, SW system) were achieved through the formation of cross-links by means of gamma-irradiation combined with thermal treatments. The effect of the incorporation of carboxymethylcellulose (CMC) and poly(vinyl alcohol) was also examined. gamma-Irradiation combined with thermal treatment improved significantly the mechanical properties, namely, puncture strength and puncture deformation, for all types of films. Irradiated formulations that contain CMC behave more similarly as elastomers. CMC showed also significant improvements of the barrier properties, namely, water vapor permeability, for irradiated films of the S system and for non-irradiated films of the SW system.