Genetic study of bolting and stem length in sugar beet (Beta vulgaris L.) using a factorial cross design

Summary The objective was to study the genetic basis of bolting and stem length in sugar beet full-sibs, developed by a factorial mating design (N.C. design II). The extent to which the inheritance of these characters can be attributed to the additive and non-dominance variation was investigated. The genetic analysis consisted of three sets (4×10, 4×8, and 3×8) of progenies. Estimates of additive genetic variance (² _A) and variance due to dominance deviations with some fractions of epistatic variance (² _D) were obtained for both characters. Bolting had a relatively large proportion of total genetic variance accounted for by ² _A. In most cases, non-additive effects were also important in determining the type of gene action in bolting. Stem length showed similar genetic variation to bolting susceptibility. Bolting resistance seemed to be dominant to the bolting susceptibility in most cases. Narrow sense heritability estimated for bolting was generally very large (0.93 to 0.96), which suggests that early generation selection for bolting resistance in a sugar beet population would be successful.     

Einfluß von Ethrel auf Form und Zuckergehalt der Zuckerrübe

Influence of Ethrel upon morphology and sugar content of sugar beet Repeated spraying of growing sugar beet plants with 100 and 200 ppm solutions of Ethrel changed the shape of the storage roots and reduced their sugar content. When CCC was added to the Ethrel solutions, the negative influence of Ethrel upon the sugar content was nearly abolished.     

Der Einfluß von Ancymidol und Ethrel auf den Gibberellinsäuregehalt (GA3) von Getreidepflanzen

Influence of Ancymidol and Ethrel on the synthesis of gibberellic acid (GA₃) in cereal plants. In pot experiments with oat, barley and rye both growth regulators Ancymidol and Ethrel considerably reduced the GA₃ content in the 3 plant species. Ancymidol was approximately 10 times more effective than Ethrel. This higher effectivity of Ancymidol on the GA₃ content and the different structure of the Ancymidol molecule compared to Ethrel makes it probable that Ancymidol inhibits the GA synthesis in a different way than Ethrel (or CCC). Although Ethrel was less effective in reducing the GA content it decreased the stalk length more than Ancymidol. It is concluded that Ethrel not only influences the GA induced growth but also other processes which regulate the length growth of plants.     

The Associations Between Tea and Coffee Drinking and Risk of Calcium-Oxalate Renal Stones

Plant Foods for Human Nutrition - Findings regarding the association between tea and coffee consumption and oxalate-calcium stone are sparse and uncertain. The purpose of this study was to...     

Inotropic and chronotropic effects of 6-hydroxy-4-methylquinolin-2(1H)-one derivatives in isolated rat atria

Background: Selective phosphodiesterase (PDE3) inhibitors improve cardiac contractility and may use in congestive heart failure. However, their proarrhythmic potential is the most important side effect. Methods: In this research, we evaluated the potential cardiotonic activity of six new synthesized selective PDE3 inhibitors (6-hydroxy-4-methylquinolin-2(1H)-one derivatives) using the spontaneously beating atria model. In each experiment, atrium of reserpine-treated rat was isolated and the contractile and chronotropic effects of a synthesized compound were assessed. The 3-isobutyl-1-methylxanthine, a non-selective PDE inhibitor, was used for comparison. Results: The results showed that, among new compounds, the best pharmacological profile was obtained with the compound 6-[4-(4-methylpiperazine-1-yl)-4-oxobutoxy]-4-methylquinolin-2(1H)-one, C6, which displayed selectivity for increasing the force of contraction (168 +/- 5% change over the control) rather than the frequency rate (138 +/- 5% change over the control) at 300 muM. However, C6 at concentrations of 10 and 100 muM produced left and upward shift in the positive inotropic concentration-response curve of isoprenaline. The -log EC50 of isoprenaline was 8.843 +/- 0.171 in the absence, 9.448 +/- 0.138 and 9.456 +/- 0.107 in the presence of 10, 100 muM of C6, respectively (P<0.001, n = 6). Also, amrinone, a selective PDE3 inhibitor, shifted the isoprenaline concentration-response curve to the left and upward. The concentration of 10 and 100 muM amrinone decreased -log EC50 of isoprenaline to 9.527 +/- 0.287 and 9.423 +/- 0.243, respectively (P<0.001, n = 6). Moreover, the positive chronotropic effect of isoprenaline was not affected by amrinone or C6. Conclusion: This study provides functional evidence for the positive inotropic effect of C6. Considering the augmentation of isoprenaline positive inotropic concentration-response with C6 and amrinone, we conclude that C6 produces its effect via potentiation of cAMP-dependent signaling system and possibly by inhibition of PDE3 activity.     

Forest Road Detection Using LiDAR Data

We developed a three-step classification approach for forest road extraction utilizing LiDAR data. The first step employed the IDW method to interpolate LiDAR point data （first and last pulses） to achieve DSM, DTM and DNTM layers （at 1 m resolution）. For this interpolation RMSE was 0.19 m. In the second step, the Support Vector Machine （SVM） was employed to classify the LiDAR data into two classes, road and non-road. For this classification, SVM indicated the merged distance layer with intensity data and yielded better identification of the road position. Assessments of the obtained results showed 63% correctness, 75% completeness and 52% quality of classification. In the next step, road edges were defined in the LiDAR-extracted layers, enabling accu- rate digitizing of the centerline location. More than 95% of the Li- DAR-derived road was digitized within 1.3 m to the field surveyed nor- rnal. The proposed approach can provide thorough and accurate road inventory data to support forest management.     

Inheritance of Bolting in Three Sugar Beet Crosses after Different Periods of Vernalization

The induction of bolting after different periods of vernalization was studied in the parental, F₁, F₂ and backcross generations (F₂× P₁ and F₂× P₂) of three crosses between five sugar beet lines. The parental lines represented different levels of bolting resistance. The populations were evaluated in replicated field or greenhouse trials. Generation mean analysis was used to quantify the genetic effects of bolting. The additive (d) parameter was predominant for those levels of cold treatment that stimulated the bolting to occur. The dominance (h) parameter was also shown to be important in most cases. In one cross, the additive × additive (i) type of epistasis was significant. The chances of detecting genetic effects are increased by exposing the plants to vernalization conditions which maximize the difference between two parental lines of each cross. Hence, it would be sensible to exploit both additive and non-additive genetic effects in any selection programme.     

Effect of Water‐Deficit Stress on Germination and Early Seedling Growth in Sugar Beet

Sugar beet progeny lines screened for both high water use efficiency and high sugar yield under drought stress conditions in the field were assessed for the rate of seed germination and early seedling growth in water deficit stress, induced by mannitol solutions. Seeds of nine different sugar beet progeny lines were grown in three experimental conditions using filter paper, perlite and water agar as substrate. Three levels of 0.0, 0.2 and 0.3 m mannitol concentrations were applied in each experiment. A factorial design was used with three replications. Germination percentage was determined in all experiments. Seedling growth parameters such as cotyledon fresh weight, cotyledon dry weight, root fresh weight, root dry weight (RDW) and root length (RL) were measured experimentally. Abnormality was only recorded in the filter paper experiment. The results showed that drought stress could be simulated by mannitol solution and significant differences were found between stress levels for seedling characteristics. Distinct genetic variances were found among progeny lines with respect to germination and early seedling growth characteristics, except for cotyledons and RDW. Seedling growth and germination rates severely declined at the highest concentration of mannitol. The rate of abnormality was increased progressively at the germination stage with an increase in mannitol concentration but it was more pronounced in the drought‐susceptible progeny lines. The highest values of relative germination % and relative growth % of RL were obtained for the most tolerant line. In conclusion, seedling characteristics, in addition to other physiological components involved in the seed germination process under specific stress conditions, may be considered for breeding purposes.     

Identifizierung und Bestimmung einiger Gibberelline und der Einfluß von Chlorcholinchlorid (CCC) auf das Gibberellinspektrum der einzelnen Getreidearten

Identification and determination of some gibberellins in the gibberellin spectrum of some cereals, als affected by CCC treatment The gibberellin contents of wheat, barley and oat plants, treated with CCC, were studied during the vegetative period. It was possible to identify a characteristic gibberellin spectrum for every cereal plant examined, although the variation was in a limited number of gibberellin forms. A great variation in the content of individual gibberellic acid forms was noticed, as well, among the different cereals studied. The highest content of gibberellins was found with GA₃. The content of GA₅ was lower than that of GA₉ while was not generally higher than that of GA₈. CCC-treated cereal plants showed lower values of gibberellins when compared with those of control. On the other hand, CCC treatment did not affect the content of gibberellins in the spikes which showed a considerably high content of GA₃, GA₅ and GA₉. A higher content of GA₇ was observed in both barley and oats, but not in the case of wheat, during tillering stage. However it was difficult, from the results of the present work, to indicate whether GA₇ is reponsible for the week effect of CCC obtained with both barley and oats or not.     

Reduced water consumption of dormant-seeded sugar beet in a semiarid climate

Dormant seeding refers to sowing spring crop species in fall. In the spring, seedlings start growing and the crop is established before spring sowing is normally completed. A dormant-seeded crop can establish quickly in spring and may escape from early season drought. A study was conducted to compare performances of dormant seeding with early and late spring seeding of sugar beet. Field experiments were carried out in the Khorasn province (NE of Iran) in 2002 and 2003 by growing a bolting resistant variety (BR1). The experiment consisted of a randomized complete block design with four replications. Dormant seeding was subjected to six irrigation treatments in addition to an early and late spring seeding. The first irrigation was applied 0, 10, 20, 30, 40 and 50 days after 60% soil moisture depletion. After harvest, root yield, sugar concentration, white sugar yield, and non-sugar components of the root samples were measured and analyzed. The results of the dormant seeding showed that root yield of sugar beet decreased with increasing delay of irrigation at the early stages of plant growth. High white sugar yield was obtained at dormant seeding without irrigation delay compared to the early seeding. No significant differences were observed for white sugar yield until three and five water withholdings in 2002 and 2003, respectively. In the second year of the experiment (2003), a relatively cold winter induced about 4.5% bolters and reduced plant population with 33% compared to the early and late spring sowing treatments. In general, white sugar yield produced in response to specific irrigation amounts for dormant-seeded treatments was higher compared to early and late seeding. However, dormant seeding cannot yet be recommended as a comprehensive agricultural practice for sugar beet and, therefore, further investigations are required to improve the quality of the beet by both agronomic techniques and new improved varieties.