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Padet Tummaruk Pannin Surapat Sutharat Sriariyakun Oraphan Seemakram Em-on Olanratmanee Rachod Tantilertcharoen Roongroj Thanawongnuwech 《Tropical animal health and production》2013,45(3):771-779
The objectives of the present study were to determine the prevalence of porcine reproductive and respiratory syndrome virus (PRRSV) in Thailand between 2005 and 2010. The study was conducted by retrospectively investigating the detection of PRRSV from different pig types including boars, sows, piglets, nursery pigs, and fattening pigs from six regions of Thailand, i.e., the northern, eastern, northeastern, central, western, and southern parts. The data were obtained from cases submitted to the Chulalongkorn University Veterinary Diagnostic Laboratory for PRRSV detection between 2005 and 2010. Frequency analyses and generalized linear models were used to evaluate the prevalence of PRRSV in relation to various factors. In total, 2,273 tissues (n?=?636), semen (n?=?210) and serum (n?=?1,427) samples were included. PRRSV was detected in 32.6 % (740/2,273) of the pigs. The virus was found in 43.1 %, 15.7 %, and 30.3 % in the tissues, semen, and serum samples, respectively (P?<?0.001). The prevalence of PRRSV was highest in 2005 (43.6 %) and lowest in 2009 (23.6 %) (P?<?0.001). The prevalence of PRRSV was highest in nursery pigs (43.7 %) and lowest in boars (15.4 %) (P?<?0.001). The prevalence of PRRSV in the hot season (34.9 %) was higher than that found in the cool season (28.1 %, P?=?0.018) but did not differ significantly compared to rainy season (34.0 %, P?=?0.486). The strain of PRRSV isolated in the present study was genotype 2 (54.5 %), genotype 1 (31.0 %), and mixed genotypes (14.5 %). It can be concluded that PRRSV was detected in the tissue samples more frequently than the semen and serum samples. The prevalence of PRRSV was high in the nursery pigs. A high prevalence of PRRSV was found in the hot season, indicating that climatic factors may also contribute to the prevalence of PRRSV in Thailand. Of all the PRRSV detected, 31.0 %, 54.5 %, and 14.5 % belonged to genotype 1, genotype 2, and mixed genotypes, respectively. 相似文献
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Acetylcholinesterase-Inhibiting Activity of Pyrrole Derivatives from a Novel Marine Gliding Bacterium, Rapidithrix thailandica 下载免费PDF全文
Yutthapong Sangnoi Oraphan Sakulkeo Supreeya Yuenyongsawad Akkharawit Kanjana-opas Kornkanok Ingkaninan Anuchit Plubrukarn Khanit Suwanborirux 《Marine drugs》2008,6(4):578-586
Acetylcholinesterase-inhibiting activity of marinoquinoline A (1), a new pyrroloquinoline from a novel species of a marine gliding bacterium Rapidithrix thailandica, was assessed (IC50 4.9 μM). Two related pyrrole derivatives, 3-(2′-aminophenyl)-pyrrole (3) and 2,2-dimethyl-pyrrolo-1,2-dihydroquinoline (4), were also isolated from two other strains of R. thailandica. The isolation of 3 from a natural source is reported here for the first time. Compound 4 was proposed to be an isolation artifact derived from 3. The two isolated compounds were virtually inactive in the acetylcholinesterase-inhibitory assay (enzyme inhibition < 30% at 0.1 g L−1). 相似文献
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Dai Kusumoto Hayato Masuya Toshihide Hirao Hideaki Goto Keiko Hamaguchi Wen-I Chou Wiwat Suasa-ard Sawai Buranapanichpan Sopon Uraichuen Oraphan Kern-asa Sunisa Sanguansub Aumporn Panmongkol Thu Pham Quang Sih Kahono Heddy Julistiono Naoto Kamata 《Journal of Forest Research》2014,19(4):404-410
Japanese oak wilt (JOW) has been prevalent in Japan since the late 1980s. Infections of the fungus, Raffaelea quercivora Kubono et Shin. Ito, which is transmitted by an ambrosia beetle [Platypus quercivorus (Murayama)], can cause JOW. Although R. quercivora, P. quercivorus, and oak trees are distributed in other Asia–Pacific countries, the incidence of JOW has not been reported outside Japan. In this study, we collected R. quercivora isolates from 5 Asian countries, including Japan, and compared their ability to induce sapwood discoloration by inoculating Q. serrata logs. The tangential widths of the discoloration in sapwoods inoculated with non-Japanese isolates were equivalent to or greater than those of the 2 Japanese isolates. This indicates that a lack of JOW incidence outside Japan is not because of the lowered ability of R. quercivora to spread discoloration compared with the Japanese isolates. Statistical analyses of the relationship between discoloration and phylogeny based on DNA sequences of actin and chitin synthase showed that the discoloration width was independent of phylogenetic relatedness among the isolates. To discuss why the occurrence of JOW has not been reported outside Japan, further studies (e.g., on host susceptibility and P. quercivorus aggression) throughout Asia are needed. 相似文献
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