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排序方式: 共有82条查询结果,搜索用时 15 毫秒
1.
H Inokuma G Nane T Uechi Y Yonahara P Brouqui M Okuda T Onishi 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(11):1225-1227
Twelve (54.5%) of 22 free-roaming dogs in Ishigaki Island had tick infestation identified as Rhipicephalus sanguineus. There were 121 ticks recovered and consisted of 28 females, 58 males, 22 nymphs and 3 larvae. Infection of dogs possibly with canine ehrlichial pathogens was examined by both indirect immunofluorescence assay and polymerase chain reaction (PCR). Two dogs of the 13 examined were sero-positive for the human granulocytic ehrlichia agent, and one of two dogs was PCR positive for Ehrlichia platys. This dog had platelet numbers slightly lower than normal value, however, no morulae were found within platelet on peripheral blood smear stained with Giemsa. 相似文献
2.
Onishi S Miyata H Inamoto T Qi WM Yamamoto K Yokoyama T Warita K Hoshi N Kitagawa H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(11):1123-1129
It is well known that some caspases in apoptosis is involved in determinant of terminal differentiation and maturation of various cells. Our previous study ultrastructurally clarified the differentiation into M cells from immature microvillous epithelial cells and the redifferentiation from M cells to microvillous epithelial cells in the follicle-associated epithelium (FAE) of rat Peyer's patch. In this study, the difference of epithelial apoptosis between the FAE of Peyer's patch and intestinal villi was immunohistochemically investigated in rat jejunoileum. As a result, cleaved caspase-3 was limited to several epithelial cells at the tip of FAE, whereas almost all of the epithelial cells were cleaved caspase-3 positive in intestinal villi. Cleaved caspase-9 was detected only in a few exfoliating or exfoliated epithelial cells of both FAE and intestinal villi. Nuclear DNA-fragmentation was detected only in several epithelial cells of the tip of FAE, while it was expressed from the middle regions in the intestinal villi. The DNase I expression of the epithelial cytoplasm was much weaker in FAE than in intestinal villi. Bcl-x expression was restricted in the apical cytoplasms of epithelial cells in the FAE, whereas it was restricted in whole cytoplasms in villous epithelial cells. These findings suggest that the progression of the apoptotic process in the epithelial cells of FAE is later than in the intestinal villi, so that the possibility of epithelial differentiation might be remained in the FAE, unlike in the intestinal villi. 相似文献
3.
Matsumoto K Inokuma H Okuda M Onishi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(1):137-140
The effects of salivary gland extract (SGE) from R. sanguineus were examined on the production of IgG1 and IgG2 and the mRNA expression of IFN-gamma, IL-2, IL-4, IL-5 and IL-10 in the mononuclear cells from canine peripheral blood, treated with concanavalin A (ConA) in vitro. SGE suppressed the ConA-induced production of IgG2. It also inhibited the expression of IFN-gamma, IL-2 and IL-5 mRNA in a dose-dependent manner. No dose-dependent suppression was observed of IL-10 mRNA expression although a significant effect was observed at a SGE protein concentration of 25 microg/ml. SGE had no effect on the mRNA expression of IL-4. These results suggest that the suppression of IgG2 production by SGE from R. sanguineus was caused by the suppression of IFN-gamma production. 相似文献
4.
I. Onishi A. Hongo T. Sasakuma T. Kawahara K. Kato H. Miura 《Genetic Resources and Crop Evolution》2006,53(5):985-992
Spelt wheat, Triticum spelta L., has been proved to be rich-sources of useful genes for tolerance to biotic and abiotic stress, and grain quality. But
this crop plant has some undesirable traits including glume tenacity and brittle rachis. Free-threshing and reduced fragility
of rachis are very important traits for cultivation. The objectives in the present study were to investigate genetic variation
of rachis fragility in a wide range of spelt accessions, to examine its genetic segregation pattern, and to clarify if rachis
fragility is associated with dosage of chromosome 5A in aneuploid lines of bread wheat. The results demonstrated that spelt
germplasm contains a wide range of rachis toughness, and thus selection of spelt wheat with desirable characteristics combined
with an appropriate level of tough rachis would be possible. Spike morphology in the F2 plants was segregated into the three types, square-headed, speltoid, and compactoid. The F2 plants with compactoid spikes had the most brittle rachis, followed by the speltoid and square-headed spike F2 plants. Rachis fragility in bread wheat also had genetic variation and was associated with dosage of chromosome 5A. 相似文献
5.
CTLA-4 control over Foxp3+ regulatory T cell function 总被引:1,自引:0,他引:1
Wing K Onishi Y Prieto-Martin P Yamaguchi T Miyara M Fehervari Z Nomura T Sakaguchi S 《Science (New York, N.Y.)》2008,322(5899):271-275
Naturally occurring Foxp3+CD4+ regulatory T cells (Tregs) are essential for maintaining immunological self-tolerance and immune homeostasis. Here, we show that a specific deficiency of cytotoxic T lymphocyte antigen 4 (CTLA-4) in Tregs results in spontaneous development of systemic lymphoproliferation, fatal T cell-mediated autoimmune disease, and hyperproduction of immunoglobulin E in mice, and it also produces potent tumor immunity. Treg-specific CTLA-4 deficiency impairs in vivo and in vitro suppressive function of Tregs-in particular, Treg-mediated down-regulation of CD80 and CD86 expression on dendritic cells. Thus, natural Tregs may critically require CTLA-4 to suppress immune responses by affecting the potency of antigen-presenting cells to activate other T cells. 相似文献
6.
7.
Inokuma H Yoshizaki Y Matsumoto K Okuda M Onishi T Nakagome K Kosugi R Hirakawa M 《Veterinary parasitology》2004,121(3-4):341-346
A total of 80 free-roaming dogs on Okinawa Island, Japan, were examined for Babesia infection using the polymerase chain reaction (PCR) and sequence analysis. Of 80 samples, 12 were positive in a Babesia genus-specific PCR. Consequent species-specific PCR for B. canis and B. gibsoni revealed that 5 (6.3%) and 7 (8.8%) dogs were infected with B. canis and B. gibsoni, respectively. Sequence analysis of the PCR products revealed that the 18S rRNA gene sequence of B. canis detected from dogs in Okinawa was very close to B. canis vogeli with sequence similarity of 99.94%. 相似文献
8.
Onishi Junya Ikeura Hiroshi Paluashova Gaukhar K. Shirokova Yulia I. Kitamura Yoshinobu Fujimaki Haruyuki 《Paddy and Water Environment》2019,17(2):185-193
Paddy and Water Environment - In the arid lands covering Uzbekistan, furrow irrigation with low application efficiency is still widely used due to a lack of funds and shortage of labor. To save... 相似文献
9.
K Ohno M Terado H Iwata H Inokuma T Onishi 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(8):915-920
Feline serum amyloid A (SAA) cDNA clone was isolated from a hepatic mRNA of a mixed-breed cat. The feline SAA cDNA clone contains 333 nucleotides and deduced amino acid sequence shows 87.4%, 73.9%, and 65.8% homology with dog, human and mouse SAA respectively. Relative to the human and mouse SAA proteins, an additional peptide of eight amino acids is specified in the feline cDNA clone. Recombinant feline SAA (rfSAA) was expressed at high levels using pGEX bacterial expression system. Cleavage from the fusion moiety, and purification using glutathione-sepharose yielded pure soluble form of rfSAA. Antibodies generated against rfSAA were specific for feline SAA and showed no cross-reactivity with human SAA. Furthermore, antibodies against human SAA did not react with feline SAA. These results indicate that antigenicity of feline SAA is totally different from human SAA. 相似文献
10.
Toshisuke Maruyama Fumikazu Noto Tsuyoshi Takahashi Kimihito Nakamura Takeo Onishi 《Paddy and Water Environment》2011,9(2):267-274
An evaluation of the nitrogen pollution load potential (NPLP) arising from several sewage treatment systems in the Tedori
River Alluvial Fan Area was evaluated by determining the removal percentage of nitrogen as a result of sewage treatment and
amounts of nitrogen lost from the different treatment systems. These data were applied to the entire area resulting in an
estimated total NPLP of 734 t year−1 (103 kg year−1), of which 548 t year−1 discharges into the Japan Sea and so does not contribute to the pollution of the environment considered here. The residual
186 t year−1 of the NPLP has a high probability of causing groundwater or downstream pollution, with 58% of the NPLP being contributed
from septic tanks not connected to public sewage systems. Rural sewage system accounted for about 18% of the NPLP. Material
directly flowing into the middle stream of the Tedori River together with sewage water from small companies and untreated
sewage water from local family dwellings made up about 3–12% of the NPLP. To improve water quality due to sewage effluent,
it is essential to increase the number of connections to public sewage systems from septic tanks. 相似文献