Interactions between different media and follicle‐stimulating hormone supplementation on in vitro culture of preantral follicles enclosed in ovarian tissue derived from collared peccaries (Pecari tajacu Linneaus, 1758)

The aim was to verify the effect of follicle‐stimulating hormone (FSH) supplementation to α‐MEM+ or TCM199+ media on the in vitro development of ovarian preantral follicles (PFs) derived from collared peccaries. Ovaries (n = 5 pairs) were collected and divided into fragments destined to control group (non‐cultured) or treatments that were cultured for 7 days. The PFs morphology, growth and activation were evaluated by classical histology. The immunohistochemistry markers Ag‐NOR and PCNA were used for nuclear proliferation analysis, and the picrosirius red labelling was used for ovarian extracellular matrix (ECM) evaluation. After 7‐day culture, only the TCM199+ treatment maintained the proportion of intact PFs similar to day 1 (63.2%), but no differences were found among treatments (p > .05). In addition, a significant increase in the growing follicles proportion was verified for all the treatments, indicating follicular activation (p > .05). By the Ag‐NOR analysis, only the TCM199+/FSH maintained the nuclear proliferation similar to the first day (p > .05). The picrosirius red staining revealed that the ECM remained intact in all the treatments (p > .05). We suggest the use of TCM199+ medium supplemented of FSH for the in vitro development of peccaries PFs under 7‐day culturing conditions.     

Deep‐water cirripedes colonizing dead shells of the cephalopod Nautilus macromphalus from New Caledonian waters

Fossil cephalopods are frequently encrusted by epibionts; however, determining whether encrustation occurred prior to or post‐mortem to the host, and whether the final environment of deposition corresponds to the habitat of encrustation is complex. The present paper describes cirripede epibionts, their calcareous bases and their attachment scars on 6 post‐mortem shells of Nautilus macromphalus, collected from deep water off New Caledonia. The cirripedes have left both cemented calcareous bases of Hexelasma and scars associated with bioerosion and discoloration produced by verrucomorph barnacles. Live cirripedes included a Metaverruca recta, with articulated opercular plates and organic tissue (on a shell that had been exposed on the sea floor for at least 150 years), and specimens of Hexelasma velutinum, one of which was partly attached to an internal surface of a shell. The disposition of verrucomorphs indicates that most Nautilus shells were colonized post‐mortem rather than during a floating stage. However, as cirripedes are known to have colonized living Nautilus, some Hexelasma, preserved only as calcareous eroded bases, may represent specimens that settled on a living Nautilus. The degree of bioerosion and discoloration induced by verrucomorph barnacles varies according to the surface preservation of Nautilus shells, with deeper and discolored traces preserved on old and degraded shells. Traces made by verrucomorphs described here are ellipsoidal and a new ichnotaxon, Anellusichnus ellipticus, is proposed to accommodate them. Importantly, verrucomorphs and other cirripede taxa with membranous bases that were attached to pristine shells may not leave any substantial scars, and, thus, will be difficult to detect in the fossil record.     

澳大利亚鸡肉生产中亟待解决的科研问题

在未来十年,驱动鸡肉行业发展的方面主要是以下几个因素:安全优质的鸡肉产品、有竞争力的价格、有利于鸡和环境的友好生产模式.尽管这些因素对于这个行业来说并不是什么新鲜意见,但是它们对于未来澳大利亚的鸡肉如何生产将变得日益重要.支持这个行业的研究项目需要根据行业的需要进行调整,并且理解和应对这些领域出现的问题.     

Use of simulation models to evaluate irrigation performance including water productivity, risk and system analyses

 Worsening water scarcity will increase pressure to use water more productively. In the classical view of irrigation research, some important aspects are often ignored: the total water balance approach, productivity of water, food security, and irrigation-system level analyses. These four approaches were evaluated using a detailed agro-hydrological model applied to an irrigation system in western Turkey. Emphasis was placed on the two dominant crops in the area: cotton and grapes. According to the classical point of view, the only result would be to irrigate the cotton with 1000 mm and the grapes with 800 mm. From the water productivity point of view, however, the water productivity of grapes appeared to be maximal without any irrigation; while for the cotton, irrigation at 600 mm maximizes water productivity. To minimize risks and increase yield stability, grapes perform better than cotton. Finally, from the irrigation system point of view, decisions can be made about the desirable cropping pattern and the distribution of water between crops. With limited amounts of water available for irrigation, a cropping pattern consisting mainly of grapes is desired; while with higher water availability, a mixture of cotton and grapes is preferable. The methods presented provide a clear methodology with which to achieve the most productive use of water. Received: 3 June 1999