微量穴醚(2.2.2)测定方法初探

用紫外分光光度法研究微量穴醚(2.2.2)的检测方法,并将该方法用于氟[18F]脱氧葡萄糖中微量穴醚(2.2.2)测定.将适量苯甲醛加入硝酸铅缓冲溶液中配制储备液,使溶液在250 nm检测波长处的吸收曲线为一平台,用储备液和微量穴醚(2.2.2)配制供试液,用紫外分光光度法检测.穴醚(2.2.2)的线性范围为1.0～10.0 μg/mL,相关系数0.999 5,平均回收率为100.5%(n=5),相对标准偏差为2.24%,方法检出限(3d)为0.15μg/mL.该方法用于氟[18F]脱氧葡萄糖中微量穴醚(2.2.2)测定,结果良好.该方法精密度高,准确性好,可用于微量穴醚(2.2.2)测定.

Abstract：

A method for the determination of trace cryptand 222 by ultraviolet spectrometry was researched. As benzaldehyde and complex of lead cryptand 222 possess the same ultralviolet absorbance peak and their maximum absorbance wavelength are both 250 nm, proper amount of benzaldehyde and lead nitrate was added to the citric acid buffer solution (pH=6.4) for preparing storage solution, and the ultralviolet absorbance curve of the storage solution was made a platform about 250 nm detection wavelength.Trace cryptand 222 was added to the storage solution, and trace complex of lead cryptand 222 was detected by ultraviolet spectrometry for quantitative determination of trace cryptand 222. This method kept in good linearity from 1.0 to 10.0 μg/mL of cryptand 222 concentration with a correlation coefficient of 0. 999 5, a recovery of 100.5% (n=5), a relative standard deviation of 2.24% and a limit of quantification of 0.15 μg/mL. This method has been successfully applied in determination of trace cryptand 222 of [18F]-fluorodeoxyglucose with satisfactory results. This method is simple, accurate and suitable for determination of trace cryptand 222.