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基于菰黑粉菌的全基因组序列,克隆得到了UeRbf1(GenBank登录号:MW375682),该基因的开放阅读框为1 281 bp,无内含子,编码426 个氨基酸,经预测具有3个C2H2锌指结构域,提示其具有转录调控功能。进一步在菰黑粉菌中敲除UeRbf1后进行体外生长及侵染试验,发现:在体外培养时,UeRbf1的缺失没有改变菌株的生长速率及单倍体形态,也未改变两个性亲和UeRbf1缺失突变体的融合及菌丝形成能力,但是融合菌丝的丝状生长能力显著减弱,而且在侵染时UeRbf1缺失突变体丧失了菌丝形成及丝状生长能力,人工接种野茭后也无法使其茎部膨大。以上结果表明,UeRbf1是调控菰黑粉菌丝状生长和侵染能力的一个重要因子。另外, UeRbf1缺失突变后,在体外融合及侵染时相关丝状生长和致病力的调控因子Biz1、Clp1、Hdp1和UeKpp6呈下调表达,其中Biz1的下调表达最为显著,在突变体中几乎不表达,说明UeRbf1对菰黑粉菌丝状生长和致病力的影响,可能是通过调控Biz1来实现的。 相似文献
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In recent years, in addition to brown spot disease, leaf black spot has occurred frequently and seriously affected the yield and quality in the production areas of Zizania latifolia in China. In order to identify the pathogen, diseased samples were collected from Lu'an, Suzhou, Tongxiang, Yuhang, Yuyao, Jinhua, Shengzhou. Finally, 90 fungal strains were obtained by tissue isolation method, in which 25 strains causing black spot disease of Z. latifolia was identified as Nigrospora oryzae by morphological observation, multiple sequence alignment analysis and pathogenicity test. Toxicity test of 12 fungicides commonly used in fields was carried out in laboratory conditions. The results showed that the effect of 250 g·L-1 prochloraz EC was best, with the EC50 of 0.002 mg·L-1; followed by 30% Pyraclostrobin SC and 60% Difenoconazole WG, with the EC50 of 0.056 and 0.057 mg·L-1, respectively. Combined with the inhibitory effect of the 12 fungicides on Ustilago esculenta, 250 g·L-1 prochloraz EC was the best choice as a control agent. This study provided scientific basis for the identification and control of leaf black spot of Z. latifolia. 相似文献
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