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Jaroslav Šnajdr Vendula Valášková Veˇra Merhautová Jana Herinková Tomáš Cajthaml Petr Baldrian 《Soil biology & biochemistry》2008,40(9):2068-2075
Extracellular lignocellulose-degrading enzymes are responsible for the transformation of organic matter in hardwood forest soils. The spatial variability on a 12 × 12 m plot and vertical distribution (0–8 cm) of the ligninolytic enzymes laccase and Mn-peroxidase, the polysaccharide-specific hydrolytic enzymes endoglucanase, endoxylanase, cellobiohydrolase, 1,4-β-glucosidase, 1,4-β-xylosidase and 1,4-β-N-acetylglucosaminidase and the phosphorus-mineralizing acid phosphatase were studied in a Quercus petraea forest soil profile. Activities of all tested enzymes exhibited high spatial variability in the L and H horizons. Acid phosphatase and 1,4-β-N-acetylglucosaminidase exhibited low variability in both horizons, while the variability of Mn-peroxidase activity in the L horizon, and endoxylanase and cellobiohydrolase activities in the H horizon were very high. The L horizon contained 4× more microbial biomass (based on PLFA) and 7× fungal biomass (based on ergosterol content) than the H horizon. The L horizon also contained relatively more fungi-specific and less actinomycete-specific PLFA. There were no significant correlations between enzyme activities and total microbial biomass. In the L horizon cellulose and hemicellulose-degrading enzymes correlated with each other and also with 1,4-β-N-acetylglucosaminidase and acid phosphatase activities. Laccase, Mn-peroxidase and acid phosphatase activities correlated in the H horizon. The soil profile showed a gradient of pH, organic carbon and humic compound content, microbial biomass and enzyme activities, all decreasing with soil depth. Ligninolytic enzymes showed preferential localization in the upper part of the H horizon. Differences in enzyme activities were accompanied by differences in the microbial community composition where the relative amount of fungal biomass decreased and actinomycete biomass increased with soil depth. The results also showed that the vertical gradients occur at a small scale: the upper and lower parts of the H horizon only 1 cm apart were significantly different with respect to seven out of nine activities, microbial biomass content and community composition. 相似文献
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Acid phosphatase and alkaline phosphatase active colonies of bacteria, isolated from forest soils, were stained. The activity
of acid and alkaline phosphatase and other soil properties (the number of aerobic bacteria, basal respiration, the level of
ammonification, the number of bacteria active in ammonification, the level of nitrification, the number of micromycetes) were
compared with the number of bacteria belonging to the genus Micrococcus. Soil samples were taken from the following horizons: F-AO1 (fermentative), H-AO2 (humic), and A (basic). The soil samples
were taken from beneath forest stands in the Izera Mountains (North Bohemia, Czech Republic). The number of acid phosphatase
active colonies correlated positively with the number of alkaline phosphatase active colonies in the F-AO1 horizon, and there
was a high, positive correlation between the former and the level of ammonification in the H-AO2 horizon. The number of alkaline
phosphatase active colonies correlated positively with organic carbon, the number of ammonification bacteria, and the number
of micromycetes in the H-AO2 horizon. The A horizon was almost biologically inactive. Neither acid nor alkaline phosphatase
activities correlated positively with the number of phosphatase active colonies of bacteria.
Received: 6 December 1996 相似文献
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The effects of simulated acid rain and acidification, combined with liming, on amylolytic, laminarinolytic and xylanolytic
activity in whole body homogenates of enchytraeids Cognettia sphagnetorum were studied under field conditions. Simulated acid rain (pH 2.5) and simulated acid rain with subsequent liming (CaCO3) were applied to experimental plots in a mixed forest soil. The pH of the soil was lowered by acid treatment (4.3), while
the pH increased after liming (6.3) in comparison with the control (4.5). Acidification of soil caused a decrease in enchytraeid
body mass and amylolytic activity. In acidified plots after liming, amylolytic activity and laminarinolytic activity increased,
while live body mass decreased. The enzymatic activity of enchytraeids depended on season and also indirectly on individual
mean mass.
Received: 12 February 1996 相似文献
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