Sediment Retention by Alternative Filtration Media Configurations in Stormwater Treatment

Urban stormwater can be treated by infiltration at the source using systems like permeable paving. A critical component of such a system is the filtration media. Laboratory experiments were conducted using columns and boxes to evaluate the sediment retention efficiencies of different filtration media—crushed Greywacke, Greywacke mixed with 10% sand, and layered Greywacke and sand-Greywacke mix. Sediments of 0.001–6 mm were applied at concentrations of 460–4,200 mg/l along with water at flow rates of 100–900 ml/min. All columns showed between 96 and 91% sediment retention efficiency for single dry sediment applications, with lowered sediment retentions at higher flow rates. Decreasing the sediment loading, applying particles of <38 μm size, and suspending the particles in inflow as opposed to directly applying sediments to the column surface gave lower sediment retention efficiencies of 55 to 89%. Sediment retention primarily occurred in the top 20 mm of all columns and the 50th percentile value of retained sediments was 100–300 μm. The box tests showed little effect of flow and sediment loading on particle retention, with the tests showing an average retention of 93%. Similar to the column tests, the box tests showed lower sediment retention (84 to 88%) for <38 μm sediments and greater retention (approximately 95%) for larger sediments.     

Pigments in avocado tissue and oil

Pigments are important contributors to the appearance and healthful properties of both avocado fruits and the oils extracted from these fruits. This study determined carotenoid and chlorophyll pigment concentrations in the skin and three sections of the flesh (outer dark green, middle pale green, and inner yellow flesh-nearest the seed) and anthocyanin concentrations in the skin of Hass avocado during ripening at 20 degrees C. Pigments were extracted from frozen tissue with acetone and measured using high-performance liquid chromatography. Pigments were also measured in the oil extracted from freeze-dried tissue sections by an accelerated solvent extraction system using hexane. Carotenoids and chlorophylls identified in the skin, flesh, and oil were lutein, alpha-carotene, beta-carotene, neoxanthin, violaxanthin, zeaxanthin, antheraxanthin, chlorophylls a and b, and pheophytins a and b with the highest concentrations of all pigments in the skin. Chlorophyllides a and b were identified in the skin and flesh tissues only. As the fruit ripened and softened, the skin changed from green to purple/black, corresponding to changes in skin hue angle, and a concomitant increase in cyanidin 3-O-glucoside and the loss of chlorophyllide a. In flesh tissue, chroma and lightness values decreased with ripening, with no changes in hue angle. The levels of carotenoids and chlorophylls did not change significantly during ripening. As fruit ripened, the total chlorophyll level in the oil from the flesh sections remained constant but declined in the oil extracted from the skin.     

Large-scale copy number polymorphism in the human genome

The extent to which large duplications and deletions contribute to human genetic variation and diversity is unknown. Here, we show that large-scale copy number polymorphisms (CNPs) (about 100 kilobases and greater) contribute substantially to genomic variation between normal humans. Representational oligonucleotide microarray analysis of 20 individuals revealed a total of 221 copy number differences representing 76 unique CNPs. On average, individuals differed by 11 CNPs, and the average length of a CNP interval was 465 kilobases. We observed copy number variation of 70 different genes within CNP intervals, including genes involved in neurological function, regulation of cell growth, regulation of metabolism, and several genes known to be associated with disease.     

Chungtaejeon,a Korean Fermented Tea,Scavenges Oxidation and Inhibits Cytokine Induced Proliferation and Migration of Human Aortic Smooth Muscle Cells

Oxidation susceptibility of serum lipid and the proliferation and migration of vascular smooth muscle cells (VSMC) from tunica media to the sub endothelial region are the key steps in the progression of atherosclerosis. The objective of this study was to determine the effects of Chungtaejeon (CTJ) on oxidation and cytokine induced proliferation and migration of human aortic smooth muscle cells (HASMC). The antioxidative effects of CTJ were evaluated by using 1,1-diphenyl-2-picryl hydrazyl (DPPH) scavenging assay, nitric oxide (NO) scavenging assay and thiobarbituric acid reactive substance (TBARS) assay. Similarly, the proliferation, migration and expression of matrix metalloproteinases (MMPs) in HASMC were assessed by MTT assay, transwell Boyden chamber assay and gelatin zymography, respectively. Western blotting was done to determine the protein expression of MMP-9, phospho extracellular regulated kinase (pERK1/2) and phospho c-Jun N-terminal kinase (pJNK). In results, the IC₅₀ values for DPPH and NO scavenging activities were 8.91 μg/ml and 14.32 μg/ml, respectively. Furthermore, CTJ inhibited TBARS formation dose dependently. The pretreatment of CTJ dose dependently inhibited the tumor necrosis factor-α (TNF-α) induced proliferation and MMP-9 expression and platelet derived growth factor (PDGF) induced migration of HASMC. Thus, CTJ can be suggested to have beneficial effect in the prevention of atherosclerosis.     

Improving androgenesis‐mediated doubled haploid production efficiency of FCV tobacco (Nicotiana tabacum L.) through in vitro colchicine application

Production of doubled haploid plants through androgenesis in flue‐cured Virginia (FCV) tobacco is a promising and convenient alternative to conventional selfing techniques for the generation of absolute homozygous lines. Here, we show a robust in vitro haploid and doubled haploid development protocol in FCV tobacco with major emphasis on improving the efficiency of chromosome doubling using in vitro colchicine treatment. We used five FCV tobacco hybrids for comparison of colchicine treatments. The anther culture response varied with developmental stages of the buds, and the highest response was observed in stage 2 buds. The effect of cold pretreatment was significant, and 4 days of pretreatment was optimum for gametic embryogenesis. Among the methods used for determining the ploidy status of plants, flow cytometry was found to be easy, fast and reliable for high‐throughput screening of haploids. Doubled haploids regeneration percentage varied from 6.77 to 11.95 in in vivo treatment, while the range of variation was 22.11% to 28.40% in in vitro colchicine treatment. We observed a pronounced increase in plant survival and the proportion of doubled haploid plants in in vitro treatment compared with the standard in vivo approach.     

Climate variability and crop production in Tanzania

Improved understanding of the influence of climate on agricultural production is needed to cope with expected changes in temperature and precipitation, and an increasing number of undernourished people in food insecure regions. Many studies have shown the importance of seasonal climatic means in explaining crop yields. However, climate variability is expected to increase in some regions and have significant consequences on food production beyond the impacts of changes in climatic means. Here, we examined the relationship between seasonal climate and crop yields in Tanzania, focusing on maize, sorghum and rice. The impacts of both seasonal means and variability on yields were measured at the subnational scale using various statistical methods and climate data. The results indicate that both intra- and interseasonal changes in temperature and precipitation influence cereal yields in Tanzania. Seasonal temperature increases have the most important impact on yields. This study shows that in Tanzania, by 2050, projected seasonal temperature increases by 2 °C reduce average maize, sorghum, and rice yields by 13%, 8.8%, and 7.6% respectively. Potential changes in seasonal total precipitation as well as intra-seasonal temperature and precipitation variability may also impact crop yields by 2050, albeit to a lesser extent. A 20% increase in intra-seasonal precipitation variability reduces agricultural yields by 4.2%, 7.2%, and 7.6% respectively for maize, sorghum, and rice. Using our preferred model, we show that we underestimate the climatic impacts by 2050 on crop yields in Tanzania by 3.6%, 8.9%, and 28.6% for maize, sorghum and rice respectively if we focus only on climatic means and ignore climate variability. This study highlights that, in addition to shifts in growing season means, changes in intra-seasonal variability of weather may be important for future yields in Tanzania. Additionally, we argue for a need to invest in improving the climate records in these regions to enhance our understanding of these relationships.     

Preparation of poly(acrylonitrile)-grafted silk fibers with antibacterial properties

This study is focused on investigating the feasibility of using silver(I) ions loaded poly(acrylonitrile)-grafted silk fibers as antibacterial dressing material. The optimum grafting conditions for ceric ammonium nitrate induced graft-copolymerization of acrylonitrile onto silk fibers were found to include initiator concentration of 35 mM, catalyst HNO₃ concentration of 0.40 M and initiation time of 10 min. The poly(acrylonitrile)-grafted silk fibers were loaded with silver(I) ions by equilibration method. The resulting fibers were investigated for their biocidal action against E. coli, by using zone inhibition and colonies counting method.     

Cooptation or solidarity: food sovereignty in the developed world

This paper builds on previous research about the potential downsides of food sovereignty activism in relatively wealthy societies by developing a three-part taxonomy of harms that may arise in such contexts. These are direct opposition, false equivalence, and diluted goals and methods. While this paper provides reasons to resist complacency about wealthy-world food sovereignty, we are optimistic about the potential for food sovereignty in wealthy societies, and we conclude by describing how wealthy-world food sovereignty can be a location of either transnational solidarity or (at least) nonharmful forms of cooptation.     

A novel pre‐emergence herbicide (Diclosulam) as an environmentally friendly weed management option in peanut and its phytotoxicity evaluation

A two‐season field experiment was conducted to evaluate the efficacy of diclosulam compared with recommended doses of pendimethalin and oxyfluorfen for weed management in peanut. Persistence of diclosulam in oil, de‐oiled cake, haulm of peanut, and soil was also studied. Diclosulam 22 and 26 g a.i. ha^?1, and a weed‐free check had a high pod yield due to more mature pods and their mass. Pre‐emergence application of diclosulam 26 g a.i. ha^?1 provided better weed management and enhanced peanut growth and yield with high net returns. Phytotoxicity studies indicated that the diclosulam application did not cause any phytotoxic symptoms (vein clearing, epinasty, hyponasty, wilting, and injury) on leaf tips and leaf surface of peanut. Diclosulam residues in peanut viz. oil, de‐oiled cake, haulm, and soil were below the detection level after harvesting of peanut from the experimental field. Further, germination percentage and seed cotton yield of cotton crop cultivated after peanut were also not affected significantly.