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1.
Ilia Michailoff 《European Journal of Forest Research》1944,66(2):120-125
Ohne Zusammenfassung 相似文献
2.
Pavel V. Panteleev Andrey V. Tsarev Victoria N. Safronova Olesia V. Reznikova Ilia A. Bolosov Sergei V. Sychev Zakhar O. Shenkarev Tatiana V. Ovchinnikova 《Marine drugs》2020,18(12)
Endogenous antimicrobial peptides (AMPs) are evolutionary ancient molecular factors of innate immunity that play a key role in host defense. Among the most active and stable under physiological conditions AMPs are the peptides of animal origin that adopt a β-hairpin conformation stabilized by disulfide bridges. In this study, a novel BRICHOS-domain related AMP from the marine polychaeta Capitella teleta, named capitellacin, was produced as the recombinant analogue and investigated. The mature capitellacin exhibits high homology with the known β-hairpin AMP family—tachyplesins and polyphemusins from the horseshoe crabs. The β-hairpin structure of the recombinant capitellacin was proved by CD and NMR spectroscopy. In aqueous solution the peptide exists as monomeric right-handed twisted β-hairpin and its structure does not reveal significant amphipathicity. Moreover, the peptide retains this conformation in membrane environment and incorporates into lipid bilayer. Capitellacin exhibits a strong antimicrobial activity in vitro against a wide panel of bacteria including extensively drug-resistant strains. In contrast to other known β-hairpin AMPs, this peptide acts apparently via non-lytic mechanism at concentrations inhibiting bacterial growth. The molecular mechanism of the peptide antimicrobial action does not seem to be related to the inhibition of bacterial translation therefore other molecular targets may be assumed. The reduced cytotoxicity against human cells and high antibacterial cell selectivity as compared to tachyplesin-1 make it an attractive candidate compound for an anti-infective drug design. 相似文献
3.
Rosado CJ Buckle AM Law RH Butcher RE Kan WT Bird CH Ung K Browne KA Baran K Bashtannyk-Puhalovich TA Faux NG Wong W Porter CJ Pike RN Ellisdon AM Pearce MC Bottomley SP Emsley J Smith AI Rossjohn J Hartland EL Voskoboinik I Trapani JA Bird PI Dunstone MA Whisstock JC 《Science (New York, N.Y.)》2007,317(5844):1548-1551
Proteins containing membrane attack complex/perforin (MACPF) domains play important roles in vertebrate immunity, embryonic development, and neural-cell migration. In vertebrates, the ninth component of complement and perforin form oligomeric pores that lyse bacteria and kill virus-infected cells, respectively. However, the mechanism of MACPF function is unknown. We determined the crystal structure of a bacterial MACPF protein, Plu-MACPF from Photorhabdus luminescens, to 2.0 angstrom resolution. The MACPF domain reveals structural similarity with poreforming cholesterol-dependent cytolysins (CDCs) from Gram-positive bacteria. This suggests that lytic MACPF proteins may use a CDC-like mechanism to form pores and disrupt cell membranes. Sequence similarity between bacterial and vertebrate MACPF domains suggests that the fold of the CDCs, a family of proteins important for bacterial pathogenesis, is probably used by vertebrates for defense against infection. 相似文献
4.
Ilia Rodushkin Emma Engstr?m Dieke S?rlin Douglas Baxter Birger H?rnfeldt Erik Nyholm Frauke Ecke 《Water, air, and soil pollution》2011,217(1-4):603-610
Chitosan is a biodegradable cationic polymer that may be a potential substitute for aluminum salts in water treatment systems. In our study, we compared the coagulation performances of chitosan with those of coagulant mixtures of chitosan and aluminum sulfate and chitosan and poly-aluminum chloride, respectively. The coagulation efficiency was evaluated in terms of coagulant dosage, solution pH, settling velocity of flocs, floc diameter, and water turbidity. The optimum dosages for acid-soluble and water-soluble chitosan required for removal of a bentonite suspension (100 NTU) were only 1.25 and 1.50 mg/l, respectively, at a respective efficiency of 99.2% and 95.8%. The optimal dosage range for water-soluble chitosan was broader than that for acid-soluble chitosan. The coagulation of bentonite decreased with increasing pH when acid-soluble chitosan was the coagulant. In contrast, the coagulation efficiency of bentonite was not affected at pH 5?C9 when water-soluble chitosan was the coagulant. The mixing of chitosan with alum or PAC in a 1:1 mass ratio significantly improved the coagulation process in terms of preventing the occurrence of re-stabilization. The highest floc settling velocity occurred at a dosage of 5?C6 mg/l of the coagulant mixtures, which was also the highest coagulation efficiency. Given the relatively high cost of chitosan and the good coagulation performance of the coagulant mixtures, we suggest that a 1:1 mass ratio of chitosan mixed with alum or PAC may be an alternative method to the use of pure chitosan in water treatment systems. 相似文献
5.
Synthetic mammalian prions 总被引:1,自引:0,他引:1
Legname G Baskakov IV Nguyen HO Riesner D Cohen FE DeArmond SJ Prusiner SB 《Science (New York, N.Y.)》2004,305(5684):673-676
Recombinant mouse prion protein (recMoPrP) produced in Escherichia coli was polymerized into amyloid fibrils that represent a subset of beta sheet-rich structures. Fibrils consisting of recMoPrP(89-230) were inoculated intracerebrally into transgenic (Tg) mice expressing MoPrP(89-231). The mice developed neurologic dysfunction between 380 and 660 days after inoculation. Brain extracts showed protease-resistant PrP by Western blotting; these extracts transmitted disease to wild-type FVB mice and Tg mice overexpressing PrP, with incubation times of 150 and 90 days, respectively. Neuropathological findings suggest that a novel prion strain was created. Our results provide compelling evidence that prions are infectious proteins. 相似文献
6.
Kholodov V. A. Yaroslavtseva N. V. Yashin M. A. Farkhodov Yu. R. Ilyin B. S. Lazarev V. I. 《Eurasian Soil Science》2021,54(3):366-371
Eurasian Soil Science - Specific features in the content and accumulation of organic carbon and nitrogen in the aggregates of typical chernozems (Protocalcic Chernozems) of long-term experimental... 相似文献
7.
ASSESSMENT OF SENTINEL LYMPH NODE METASTASIS IN CANINE MAMMARY GLAND TUMORS USING COMPUTED TOMOGRAPHIC INDIRECT LYMPHOGRAPHY 下载免费PDF全文
Christina Soultani Michail N. Patsikas Maria Karayannopoulou Samuel Jakovljevic Ioannis Chryssogonidis Lysimachos Papazoglou Nikolaos Papaioannou Paraskevi Papadopoulou Kyriaki Pavlidou Georgia M. Ilia Dimitrios G. Kaitzis Tatiana M. Ilia 《Veterinary radiology & ultrasound》2017,58(2):186-196
In the staging process of the breast cancer, demonstrating metastasis of the sentinel lymph node (SLN) has an important prognostic value, in both humans and animals. The aim of this prospective case‐control study was to determine the diagnostic value of computed tomographic indirect lymphography (CT‐LG) for detecting SLN metastasis in dogs with mammary cancer. Thirty‐three female dogs with tumors in the abdominal and inguinal mammary glands were prospectively selected and subjected to CT‐LG, 1 and 5 min after injection of 1 ml of contrast agent (iopamidol) in the subareolar tissue of the neoplastic and the contralateral normal mammary glands. The pattern of postcontrast opacification, degree of postcontrast enhancement, and size and shape were assessed in 65 SLNs in total and were correlated with histopathological findings. The absence of opacification or heterogeneous opacification 1 min after contrast medium injection showed the highest sensitivity, specificity, and accuracy (93%, 100%, and 98.4%, respectively). In images taken 1 min after injection, an absolute density value lower than 444 Hounsfield units (HU) in the center of the SLN also provided significant sensitivity and specificity (93.8% and 75%, respectively). The size and shape of the SLN (maximum and minimum diameter, maximum/minimum diameter ratio, maximum diameter/height of fifth thoracic vertebral body ratio) showed the lowest sensitivity and specificity. Results of this study support the hypothesis that CT‐LG could help in the assessment of SLN metastasis in cases of mammary gland tumors in dogs. 相似文献
8.
Lohmüller T Aydin D Schwieder M Morhard C Louban I Pacholski C Spatz JP 《Biointerphases》2011,6(1):MR1-M12
This comprehensive overview of block copolymer micelle nanolithography (BCMN) will discuss the synthesis of inorganic nanoparticle arrays by means of micellar diblock copolymer approach and the resulting experimental control of individual structural parameters of the nanopattern, e.g., particle density and particle size. Furthermore, the authors will present a combinational approach of BCMN with conventional fabrication methods, namely, photolithography and electron beam lithography, which combines the advantages of high-resolution micronanopatterning with fast sample processing rates. In addition, the authors will demonstrate how these nanoparticle assemblies can be transferred to polymer substrates with a wide range of elasticity. In the second part of this report the authors will introduce some of the most intriguing applications of BCMN in biology and materials science: The authors will demonstrate how nanoparticle arrays may be used as anchor points to pattern functional proteins with single molecule resolution for studying cellular adhesion and present a technological roadmap to high-performance nanomaterials by highlighting recent applications for biomimetic optics and nanowires. 相似文献
9.
Arpigny C Jehin E Manfroid J Hutsemékers D Schulz R Stüwe JA Zucconi JM Ilyin I 《Science (New York, N.Y.)》2003,301(5639):1522-1524
High-resolution spectra of the CN B2 summation operator +-X2 summation operator + (0,0) band at 390 nanometers yield isotopic ratios for comets C/1995 O1 (Hale-Bopp) and C/2000 WM1 (LINEAR) as follows: 165 +/- 40 and 115 +/- 20 for 12C/13C, 140 +/- 35 and 140 +/- 30 for 14N/15N. Our N isotopic measurements are lower than the terrestrial 14N/15N = 272 and the ratio for Hale-Bopp from measurements of HCN, the presumed parent species of CN. This isotopic anomaly suggests the existence of other parent(s) of CN, with an even lower N isotopic ratio. Organic compounds like those found in interplanetary dust particles are good candidates. 相似文献
10.
Winfried Schröder Marcel Holy Roland Pesch Harry Harmens Ilia Ilyin Eiliv Steinnes Renate Alber Yuliya Aleksiayenak Oleg Blum Mahmut Coşkun Maria Dam Ludwig De Temmerman Marina Frolova Marina Frontasyeva Laura Gonzalez Miqueo Krystyna Grodzińska Zvonka Jeran Szymon Korzekwa Miodrag Krmar Eero Kubin Kestutis Kvietkus Sébastien Leblond Siiri Liiv Sigurður Magnússon Blanka Maňkovská Juha Piispanen Åke Rühling Jesus Santamaria Zdravko Spiric Ivan Suchara Lotti Thöni Viktor Urumov Lilyana Yurukova Harald G. Zechmeister 《Journal of Soils and Sediments》2010,10(8):1572-1584