Comparison of genetic variation among accessions of <Emphasis Type="Italic">Aegilops tauschii</Emphasis> using AFLP and SSR markers

Genetic diversity of 54 accessions of Aegilops tauschii from five countries was assessed using sequence-tagged microsatellites (or simple sequence repeats, SSRs) and amplified fragment length polymorphisms (AFLPs). In the case of AFLP analysis, a total of 256 amplification products obtained, 234 of them were polymorphic across all the 54 accessions. A total of 224 fragments were obtained from the 24 SSR primers and 219 of fragments were polymorphic across all the genotypes screened. Based on both AFLP and SSR markers, the highest percentage of polymorphisms were obtained in Iranian and accessions of unknown origin. The highest polymorphic information content (PIC) value was observed for SSRs (0.82) while the highest marker index (MI) value was for AFLPs (8.5) reflecting the hyper-variability of the first and the distinctive nature of the second system. Principal co-ordinate analysis (PCO) revealed congruent patterns of genetic relationships for both data sets, but did not group accessions strictly according to their geographical origins. Poor correlation was found between AFLP and SSR marker loci. This low association may be due to low number of AFLP and SSR markers. These results show that molecular markers can help to organize the genetic variability and expose useful diversity for breeding purposes.     

Population structure,genetic diversity,and sexual state of the rice brown spot pathogen Bipolaris oryzae from three Asian countries

Bipolaris oryzae causes brown spot in rice (Oryza sativa) inflicting substantial grain yield losses worldwide. Knowledge of the population structure, genetic diversity and sexual recombination of the fungal pathogen can help to implement effective disease management strategies. In this study, B. oryzae isolates sampled from Iran, the Philippines and Japan were analysed with 12 simple‐sequence repeat (SSR) markers, newly developed from the genome sequence of the fungus. Among the 288 B. oryzae isolates genotyped, 278 unique haplotypes were identified. High genotype numbers (richness) with even distribution (evenness) were found within the collection sites. Both mating types, MAT1‐1 and MAT1‐2, were present in each collection area, and the sexual state was induced under controlled conditions with production of viable ascospores. However, the tests of linkage disequilibrium rejected of the hypothesis of random mating. Discriminant analysis of principal components (DAPC) revealed that the B. oryzae collection formed three clusters, each consisting of isolates from different collection sites. Analysis of molecular variance (amova ) showed that genetic variation among clusters was 18.7%, with the rest of the variation distributed within clusters (R_ST = 0.187, P < 0.001). Statistically significant pairwise genetic differentiation was found between the clusters. These results show that Asian B. oryzae isolates are genetically diverse, and, overall, distributed in three groups. These findings will be helpful in managing the disease and guide the use of representative isolates needed for selection of resistant rice varieties.     

Evaluation of growth indices and quantitative traits of safflower under organic farming and conventional agriculture

Application of organic fertilizers in sustainable agriculture systems improves yield sustainability of field crop production. The current research has been formed to investigate the effects of various levels of vermicompost (zero, 3, 6 and 9 t ha^?1) in combination with foliar spraying of potassium humate (0, 1, 2 and 3 mL L^?1) on spring safflower, in Iran during 2012–2013. In addition, inorganic fertilization has been considered as conventional agriculture (CA). In the current experiment, growth indices, seed yield, yield components and flower yield were evaluated. The results showed that the maximum leaf area index, total dry weight and crop growth rate have been determined at 9 t ha^?1 vermicompost and 3 mL L^?1 K-humate while the maximum netto assimilation rate has existed in CA at the emergence of flower buds. Likewise, the results indicated that vermicompost leads to a significant increase in seed yield, flower yield and yield components except 1000 seed weight. Flower yield, head number per plant and seed number per head were affected by K-humate concentrations and increased significantly from 1 to 3 mL L^?1. It should be mentioned that 9 t ha^?1 vermicompost and 3 mL L^?1 K-humate produced the highest seed and flower yield.     

Effect of saline irrigation water on agronomical and phytochemical characters of chamomile (Matricaria recutita L.)

In arid and semi-arid regions, where water availability is a major limitation in crop production, using alternative water resources, such as saline water is one way to utilize lands. Chamomile (Matricaria recutita L.) as an annual medicinal herb may be considered as an economic substitute for field crops irrigated with fresh water since it has adaptability to wide range of climate and soil. A field examination was conducted during 2004–2005 using complete randomized block design with four replications in order to evaluate the effects of saline irrigation water on morphological characters, mineral content, oil quantity (content, yield), oil composition and apigenin content of chamomile. In each plot, 0.6 g/m² of seeds were grown in 4 rows. The irrigation water had five different salinity levels (0, 4, 8, 12 and 16 dS m⁻¹). The investigated characters through cultivation were fresh weight of flower (g), dry weight of flower (g), dry weight of aerial stems (g), dry weight of root (g), oil yield (kg/h), oil content (%), oil quality and apigenin content (%). After harvesting, the content of minerals (Na⁺, Cl⁻, K⁺, Ca²⁺, Mg²⁺) were evaluated in aerial parts and roots of each plot. Mean comparisons for fresh flower weight in different treatments showed that fresh flower yield decreased with increasing salinity and it was higher in control compared to others. Analysis of variance showed that saline irrigation water had no significant effect on oil quantity (yield and content), oil quality (chemical composition) or apigenin content. Our results showed that chamomile is able to maintain all its medical properties, under saline condition and could be cultivated economically in such conditions.     

A Nondestructive,Remote Sensing-Based Estimation of the Economic Value of Aboveground Temperate Forest Biomass (Case Study: Hyrcanian Forests,Nowshahr-Iran)

ABSTRACT

Background: Traditional field-based methods of measurement of biomass and carbon storage face difficulty in collecting time-consuming and expensive, suggests the use of remote sensing-based techniques. It estimates the economic value of the aboveground biomass (AGB) using satellite remote sensing across the Hyrcanian forests of Iran. Methods: The Landsat-8 OLI sensor data were combined with field-based allometric information of 186 circular sample plots. The AGB was calculated at the plot level using the collected data and specific volumetric mass for species in the studied area. It was followed by calculating the carbon storage using a 50% carbon coefficient and the photosynthesis equation at the forest parcel level. Model results using the random forest and support vector machines. The carbon sequestration value was calculated with USD 25.3 as a shadow value of carbon in 2014 and using the replacement cost approach. Results: The highest performances achieved by RF for biomass, carbon storage and the carbon storage value (Iranian Rials of 0.67% and 16%, respectively). The value was derived once at the plot level of 12.22 million IRR (370.43 USD) per ha. In addition, at the parcel level, which resulted in an estimated value of 12.87 million IRR (390.24 USD) per ha.     

Morphological and oil content variations amongst Damask rose (Rosa damascena Mill.) landraces from different regions of Iran

Forty landraces of Damask rose were collected from 28 provinces (13 origin sites) in Iran and evaluated to determine the diversity among them. The investigated characters were: flower weight, flower diameter, peduncle length, number of petals, number of stamens and oil content. Pearson's coefficients showed that number of petals was positively correlated with flower weight (r = 0.642) and number of stamens (r = 0.630), while its correlation with peduncle length was negative (r = −0.53). A negative correlation was obtained between oil content and number of stamens (r = −0.60) which is useful for indirect selection of landraces with low number of stamens and therefore high amount of oil content. Cluster analysis based on five botanical characters and oil content separated the origin site 12, which included Guilan, Mazandaran and Golestan provinces with a specific climate condition of temperate humid, from other origin sites. This distinctiveness was already confirmed by the bi-plot of the principal components. The distribution of Damask rose landraces provided useful information for the future collection and for the genetic resource management in these origin sites.     

Genetic variability of seed-quality traits in gamma-induced mutants of sunflower (<Emphasis Type="Italic">Helianthus annuus</Emphasis> L.) under water-stressed condition

Sunflower is one of the major annual world crops grown for edible oil and its meal is a potential source of protein for human consumption. It contains tocopherol that decreases potential risk of chronic diseases in human. The objectives of the current research are to assess the genetic variability and to identify AFLP markers and candidate genes associated with seed-quality traits under well-irrigated and water-stressed conditions in gamma-induced mutants of sunflower. Two mutant lines, M8-826-2-1 and M8-39-2-1, with significant increased level of oleic acid were identified that can be used in breeding programs for quality increase high oxidative stability and heart-healthy properties. The significant increased level of tocopherol in mutant lines, M8-862-1N1 and M8-641-2-1, is justified by observed polymorphism for tocopherol pathway-related gene; MCT. The most important marker for total tocopherol content is E33M50_16 which explains 33.9% of phenotypic variance. One of the most important candidate genes involving fatty acid biosynthesis, FAD2 (FAD2-1), is linked to oleic and linoleic acids content and explained more than 53% of phenotypic variance. Common markers associated with different seed-quality traits in well-irrigated and water-stressed conditions could be used for marker-assisted selection (MAS) in both conditions. Other markers, which are specific for one condition whereas linked to different traits or specific for a trait, could be useful for a given water treatment.     

Distribution and diversity of Aegilops tauschii in Iran

The wide morphological variation of Aegilops tauschii has led to the distinction of different subspecies; a typical ssp. tauschii and a second ssp. strangulata. However some researchers pointed out the existance of the intermediate form among morphologically distinguished subspecies. Distribution, diversity and the relationship between different subspecies and the intermediate form were evaluated in the Iranian Ae. tauschii collection. This collection was classified to 15 different populations according to morphological similarities and the collecting origin of accessions. The highest variation was found in tauschii population of Golestan followed by tauschii populations of Gilan and Ardebill, whereas the lowest variation was observed in tauschii populations of central Iran. Two discriminant functions suggested that the length of rachis node and spikelet glume, particularly, the length/width ratios of these traits had the highest impact on identification of different forms. Mahalanobis distances (D ² ) between the two subspecies along with intermediate form on the multidimensional scaling plot showed that the intermediate form is more similar to ssp. tauschii than ssp. strangulata. Although, the diversity within the ssp. strangulata was not very high, it widely affected the diversity of Iranian accessions of Ae. tauschii through continues crossing with the more diversed subspecies, tauschii, during thousands of years. This fact had lead to expansion of its distribution from its origin to Northern Khorasan, Northern Semnan and Eastern Ardebill by producing the intermediate form.