First record of autochthonous canine ehrlichiosis caused by Ehrlichia canis in Romania

This case study describes the first genetically confirmed and clinically manifested autochthonous Ehrlichia canis infection in a 9‐year‐old female mixed‐breed dog from Romania. Health screening of the dog included clinical examination, evaluation of stained peripheral blood smear and hematologic variables, as well as serologic testing and molecular analysis. Clinical signs included fever, apathy, dehydration, pale mucous membranes, and weakness. The microscopic examination of the blood smear and immunologic assays for Borrelia burgdorferi, Anaplasma phagocytophilum, and E canis antibodies, and for Dirofilaria immitis antigen yielded negative results. Hematologic abnormalities included moderate nonregenerative anemia, leucopenia with neutropenia, and moderate thrombocytopenia. The biochemical abnormalities identified were hypoalbuminemia, and mildly increased serum enzyme activities of AST and ALT. In addition, increased urea and creatinine levels associated with low urine specific gravity and proteinuria were also present. Nested PCR amplification of the partial E canis 16S rRNA gene demonstrated the presence of this rickettsial pathogen in the dog's blood, which subsequently was confirmed through sequencing based on the 100% homology with GenBank deposited E canis isolates. After specific treatment with doxycycline (10 mg/kg, orally, SID) for one month, the proteinuria, and hematologic and serum biochemical abnormalities with the exception of mild azotemia resolved. This report supports the geographical expansion of canine ehrlichiosis caused by E canis in nonendemic regions of Europe.     

Oxidant-antioxidant imbalance in horses infected with equine infectious anaemia virus

This study assesses the impact of equine infectious anaemia virus (EIAV) infection on the oxidant/antioxidant equilibrium of horses. Blood samples from 96 Romanian horses aged 1-25 years, were divided into different groups according to their EIAV-infection status, age, and time post-seroconversion. The effect of infection on oxidative stress was estimated by measuring enzymatic antioxidants (superoxide dismutase [SOD], glutathione peroxidase [GPx] and catalase), non-enzymatic antioxidants (uric acid and carotenoids), and lipid peroxidation (malondialdehyde [MDA]). Infection modified the oxidant/antioxidant equilibrium in the horses, influencing GPx and uric acid levels (P<0.05). Time post-seroconversion also contributed to oxidative stress imbalance, exhibiting a significant influence on both SOD and MDA concentrations in the blood (P<0.05). Animal age did not have a significant influence on oxidative stress. Recently infected horses (<1 year following seroconversion), and horses >5 years old, represented the most vulnerable category in terms of oxidative stress, followed by recently infected animals <5 years old. The results of this study are novel in implicating EIAV infection in the development of oxidative stress in horses.     

Biological indicators for evaluating soil quality improvement in a soil degraded by erosion processes

Journal of Soils and Sediments - Erosion is one of the main soil degradation problems. It diminishes soil biological activity and therefore its quality. The aim of the study was to evaluate if the...     

Temporal variation in the recovery from impairment in adriamycin-induced wound healing in rats

Background  

An adriamycin-induced impairment of wound healing has been demonstrated experimentally in rats. The purpose of this study is to investigate a possible temporal variation in recovery from the impairment of wound healing caused by adriamycin administration.     

Analysing agricultural users’ patterns of behaviour: The case of OPTIRas™, a decision support system for starch crop selection

Redesigning IT systems for specific user groups encompasses a lot of effort with respect to analysing and understanding user behaviour. The goal of this paper is to provide insights into patterns of behaviour of agricultural users, during the usage of a decision support system called OPTIRas™. This system aids agricultural users in their cultivar selection activities. We analyse logs resulting from OPTIRas™, and we get insights into user’s navigational patterns. We claim that the results of our analysis can be used to support the redesign of decision support systems in order to address specific agricultural users’ characteristics.     

Über die Wirkung von Bacillus thuringiensis in Kombination mit dem synthetischen Pyrethroid Ambusch auf Operophthera brumata,Tortrix viridana und die Insektenfauna eines Eichenbestandes

In order to increase the effect of the biopreparation Thuricide HP, we mixed it with pyrethroid Ambusch, a substance highly effective against insects possessing satisfactory parameters as regards toxic residua. Using aerial application, we tested Thuricide HP as a 0.5% suspension in a dose of 1 kg in 200 l water per one ha, the same dose of the preparation combined with a sublethal dose of Ambusch (8 ml/ha, i. e., 2 g of effective substance), and Ambusch in a dose of 120 ml/ha, i. e., 30 g of effective substance/1 ha. The mortality of larvae ofO. brumata andT. viridana attained 61% after the application of Thuricide HP, 83% after Thuricide HP combined with Ambusch, 96% after treatment with Ambusch. As indicated by our results, the biopreparation killed only larvae of Lepidoptera, while pyrethroid effected the entire spectrum of insect species colonizing the crowns of the treated stand. A combination of the biopreparation with a sublethal dose of pyrethroid was sufficiently effective against the pest, with a decreased effect on the remaining insect species.