Livestock water productivity in mixed crop–livestock farming systems of the Blue Nile basin: Assessing variability and prospects for improvement

Water scarcity is a major factor limiting food production. Improving Livestock Water Productivity (LWP) is one of the approaches to address those problems. LWP is defined as the ratio of livestock’s beneficial outputs and services to water depleted in their production. Increasing LWP can help achieve more production per unit of water depleted. In this study we assess the spatial variability of LWP in three farming systems (rice-based, millet-based and barley-based) of the Gumera watershed in the highlands of the Blue Nile basin, Ethiopia. We collected data on land use, livestock management and climatic variables using focused group discussions, field observation and secondary data. We estimated the water depleted by evapotranspiration (ET) and beneficial animal products and services and then calculated LWP. Our results suggest that LWP is comparable with crop water productivity at watershed scales. Variability of LWP across farming systems of the Gumera watershed was apparent and this can be explained by farmers’ livelihood strategies and prevailing biophysical conditions. In view of the results there are opportunities to improve LWP: improved feed sourcing, enhancing livestock productivity and multiple livestock use strategies can help make animal production more water productive. Attempts to improve agricultural water productivity, at system scale, must recognize differences among systems and optimize resources use by system components.     

Conservation of marker synteny during evolution

Summary An aspect of cereal science that is becoming increasingly important is comparative genetics. Establishment of the relationship between genomes within polyploids, between species within tribes and between species within families will allow not only the integration of genetic maps but also the knowledge acquired of each of the species. Using a set of homoeologous probes, workers found the relationship between the three wheat genomes to be precisely collinear, after taking a few major translocation events into account. Transfer of the wheat map to rye led to the elucidation of similar relationships between the three wheat genomes and that of rye. Genome collinearity, however, extends even beyond tribes. In a comparison of the genomes of wheat, rice and maize, it was shown that despite the separation of these genomes for possibly 50 million years, gene order was still highly conserved. This collinearity between genomes can be exploited in a number of ways.     

Abatement of High Concentrated Ammonia Loaded Waste Gases in Compost Biofilters

The performance of lab-scale compostbiofilters for the purification of waste gasescontaining high (>70 mg m^-3) ammoniaconcentrations was studied. When using fresh compostmaterial, no effect of inoculating the compostmaterial with a nitrifying culture was observed sincehigh elimination capacities (up to 350 gNH₃ m^-3 d^-1) were obtained in both theinoculated and the non-inoculated biofilter. Due tothe physico-chemical interaction of NH₃ with thecompost material at the start of the experiment, nomicrobiological start-up period was observed and highremoval efficiencies were obtained from the first dayon. Next to this, no NH₃-toxicity was observedeven at concentrations up to 550 mg NH₃ m^-3.About 50% of the NH₃-removal was found to benitrified, while the other 50% remained in thebiofilter as NH ⁺₄ . As a result of this, noacidification of the carrier material was observed andNH₄NO₃ accumulated in the biofilter. Due toosmotic effects, however, a complete inhibition innitrification and NH₃-removal was obtained at ameasured NH₄NO₃-concentration in the compostmaterial of 6–7 g N kg^-1, corresponding to acumulative NH₃-removal in the biofilter of ±6000 g m^-3. Finally, it was illustrated that theremoval of the odorant dimethyl sulfide (Me₂S) ina Hyphomicrobium MS3-inoculated compostbiofilter is completely inhibited due toNH₃-toxicity at a waste gas concentration of 100 mg NH₃ m^-3. Next to this, theNH ⁺₄ - and NO ^?₃ -concentrations inthe compost material that were shown to inhibit thenitrification, also strongly affected theMe₂S-degrading activity of Hyphomicrobium MS3.

     

Determinants of annual fluxes of CO2 and N2O in long-term no-tillage and conventional tillage systems in northern France

The greenhouse gases CO₂ and N₂O emissions were quantified in a long-term experiment in northern France, in which no-till (NT) and conventional tillage (CT) had been differentiated during 32 years in plots under a maize–wheat rotation. Continuous CO₂ and periodical N₂O soil emission measurements were performed during two periods: under maize cultivation (April 2003–July 2003) and during the fallow period after wheat harvest (August 2003–March 2004). In order to document the dynamics and importance of these emissions, soil organic C and mineral N, residue decomposition, soil potential for CO₂ emission and climatic data were measured. CO₂ emissions were significantly larger in NT on 53% and in CT on 6% of the days. From April to July 2003 and from November 2003 to March 2004, the cumulated CO₂ emissions did not differ significantly between CT and NT. However, the cumulated CO₂ emissions from August to November 2003 were considerably larger for NT than for CT. Over the entire 331 days of measurement, CT and NT emitted 3160 ± 269 and 4064 ± 138 kg CO₂-C ha⁻¹, respectively. The differences in CO₂ emissions in the two tillage systems resulted from the soil climatic conditions and the amounts and location of crop residues and SOM. A large proportion of the CO₂ emissions in NT over the entire measurement period was probably due to the decomposition of old weathered residues. NT tended to emit more N₂O than CT over the entire measurement period. However differences were statistically significant in only half of the cases due to important variability. N₂O emissions were generally less than 5 g N ha⁻¹ day⁻¹, except for a few dates where emission increased up to 21 g N ha⁻¹ day⁻¹. These N₂O fluxes represented 0.80 ± 0.15 and 1.32 ± 0.52 kg N₂O-N ha⁻¹ year⁻¹ for CT and NT, respectively. Depending on the periods, a large part of the N₂O emissions occurred was probably induced by nitrification, since soil conditions were not favorable for denitrification. Finally, for the period of measurement after 32 years of tillage treatments, the NT system emitted more greenhouses gases (CO₂ and N₂O) to the atmosphere on an annual basis than the CT system.     

Biological control of Rhizoctonia root rot on bean by phenazine- and cyclic lipopeptide-producing Pseudomonas CMR12a

Pseudomonas CMR12a was previously selected as an efficient biocontrol strain producing phenazines and cyclic lipopeptides (CLPs). In this study, biocontrol capacity of Pseudomonas CMR12a against Rhizoctonia root rot of bean and the involvement of phenazines and CLPs in this ability were tested. Two different anastomosis groups (AGs) of Rhizoctonia solani, the intermediately aggressive AG 2-2 and the highly aggressive AG 4 HGI, were included in growth-chamber experiments with bean plants. The wild-type strain CMR12a dramatically reduced disease severity caused by both R. solani AGs. A CLP-deficient and a phenazine-deficient mutant of CMR12a still protected bean plants, albeit to a lesser extent compared with the wild type. Two mutants deficient in both phenazine and CLP production completely lost their biocontrol activity. Disease-suppressive capacity of CMR12a decreased after washing bacteria before application to soil and thereby removing metabolites produced during growth on plate. In addition, microscopic observations revealed pronounced branching of hyphal tips of both R. solani AGs in the presence of CMR12a. More branched and denser mycelium was also observed for the phenazine-deficient mutant; however, neither the CLP-deficient mutant nor the mutants deficient in both CLPs and phenazines influenced hyphal growth. Together, results demonstrate the involvement of phenazines and CLPs during Pseudomonas CMR12a-mediated biocontrol of Rhizoctonia root rot of bean.     

Serum thyroid hormone concentrations and thyroglobulin autoantibodies in trained and non-trained healthy whippets

Numerous factors including non-thyroidal systemic diseases and drug administration can significantly alter canine thyroid function test results. Furthermore, the importance of breed specific variations has probably been underestimated. In this study, total thyroxine (TT4), free thyroxine (FT4), canine endogenous thyroid stimulating hormone (cTSH) serum concentrations and thyroglobulin autoantibodies (TgAA) were determined in a population of healthy whippets and compared to a control group of different breeds. Mean TT4 values were significantly lower in the whippets but no significant differences were seen between whippets and control dogs for FT4 and for cTSH. The prevalence of serum TgAA in the whippets was 2%, and this was not significantly different from the controls. The results suggest a breed variation for TT4, but not for FT4, cTSH and TgAA serum concentrations in whippets. Serum thyroid hormone concentrations were also compared between trained and non-trained whippets and it was concluded that regular training did not seem to have any significant influence.     

Sentinel lymph node mapping by near‐infrared fluorescence imaging and contrast‐enhanced ultrasound in healthy dogs

Sentinel lymph node (SLN) mapping is a valuable and crucial diagnostic procedure in staging malignancies. We compared two non‐invasive techniques, near‐infrared (NIR) fluorescence imaging and contrast‐enhanced ultrasound (CEUS), to identify the SLNs in three superficial anatomical regions in an animal model. Six healthy laboratory dogs were included in a proof‐of‐concept trial. A NIR fluorescent dye (Indocyanine Green) and microbubbles (Sonovue) were consecutively injected subdermally in the Inguinal, axillary and popliteal region to map the SLNs. Transcutaneous NIR fluorescence imaging identified SLNs in 17 out of a total of 18 occasions. CEUS identified SLNs in all regions (18/18). Whereas NIR fluorescence imaging performed better in the visualization of the afferent lymphatic tract, CEUS demonstrated different filling patterns of the SLNs, a feature potentially critical for the concept of SLN mapping in cancer patients. Both NIR fluorescence imaging and CEUS are safe, non‐invasive, practical and accurate methods to perform real‐time transcutaneous SLN mapping with potential in a clinical setting.     

Home garden system dynamics in Southern Ethiopia

Home gardens in southern Ethiopia are regarded as efficient farming systems, allowing interactions and synergies between crop, tree and livestock components. However, these age-old traditional home gardens are evolving rapidly in response to changes in both the socio-economic and biophysical environment. Altered cropping patterns, farm size and component interactions may affect the systems’ sustainability. Home gardens exhibit a huge diversity in farms and farming systems, which needs to be understood in order to design interventions for improvement. Dynamics of home gardens were studied over two-decades (1991–2013) based on a survey of 240 farm households and focus group discussions. Farms were grouped into five types: Khat-based, Enset-cereal-vegetable, Enset-based, Enset-coffee and Enset-livestock. Farm trajectories revealed a shift from food-oriented Enset-based and Enset-livestock systems to (1) cash crop oriented khat-based systems, and (2) combined food and cash crop oriented Enset-cereal-vegetable systems. In densely populated, market proximate areas a major trend was expansion of khat, from 6 to 35% of the area share per farm, while the combined area share of enset and coffee decreased from 45 to 25%. Concurrently, the cattle herd size fell from 5.8 TLU to 3.9 TLU per household. In medium populated, less accessible areas the trend was consolidation of combined production of food and cash crops. Enset and coffee together maintained a share of over 45%. Easy transport and marketing of the perishable cash-generating khat compared with traditional crops favoured its cultivation among smallholders located close to markets. The insights in home garden change in response to increasing population pressure, decreasing farm size and market development may help to design interventions to increase system sustainability.