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1.
Peru Gopal BISWAS Yuma OHARI Uday Kumar MOHANTA Tadashi ITAGAKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):666
We analyzed the nuclear ribosomal internal transcribed spacer (ITS) 1 and ITS2 sequences for Bangladesh isolates of Ascaridia galli, and we determined that the sequences were unreliable as molecular markers for distinguishing A. galli from other Ascaridia species, because the sequences showed high identity with that of A. columbae. However, the ITS1 sequences were available for designing PCR primers distinguishable between Ascaridia galli and Heterakis spp. Bangladesh isolates of A. galli constituted a monophyletic clade along with other geographical isolates in the cytochrome c oxidase subunit I (COI) phylogenetic tree, however, we could not clarify the phylogenetic relationships between A. galli and other Ascaridia spp., because their available sequences in GenBank were very few. The developed PCR method using DNA from A. galli and Heterakis spp. eggs would enable differential diagnosis of the individual infections in the future. 相似文献
2.
The activity levels of succinate dehydrogenase (SDH) and glucose‐6‐phosphate dehydrogenase (G6PD) were assessed in various tissues of Cyprinus carpio var communis which had been exposed to lethal concentrations of group‐II pyrethroids (deltamethrin, cypermethrin, fenvalerate and fluvalinate) for a period of 72 h. The results indicated a steady decrease in SDH activity with a concomitant increase in G6PD activity. The decreased SDH activity indicated inhibition of SDH at mitochondrial level and the increased G6PD activity an enhancement of an alternative pathway of carbohydrate metabolism, viz the hexose monophosphate shunt (HMP) or pentose phosphate pathway as a biochemical adaptation to overcome the toxic stress. © 2001 Society of Chemical Industry 相似文献
3.
Successful fruit set depends on several reproductive processes including pollen germination and tube growth processes. An experiment was conducted to determine the effects of temperature on pollen germination characteristics and to identify species/genotypic differences in Capsicum using the cumulative temperature response index (CTRI) concept. Pollen was collected from plants of seven genotypes from five Capsicum species, adapted to various parts of the world and grown outdoors in large pots. The pollen was subjected to in vitro temperatures ranging from 15 to 50 °C at 5 °C intervals. Pollen germination and tube lengths were recorded for all species after 24 h of incubation at the respective treatments. Species/genotypes differed significantly for in vitro pollen germination percentage and pollen tube length with mean values of 78% and 734 μm, respectively. The mean cardinal temperatures (Tmin, Topt, and Tmax) averaged over genotypes, were 15.2, 30.7, and 41.8 °C for pollen germination and 12.2, 31.2, and 40.4 °C for pollen tube growth. The CTRI of each species/genotype calculated as the sum of eight relative individual stress response values, such as maximum pollen germination, maximum pollen tube length; Tmin, Topt, and Tmax temperatures of pollen germination, and pollen tube lengths, identified species tolerance to high temperatures. Capsicum annum cv. Mex Serrano from Mexico was identified as tolerant, C. chacoense cv. 1312 and C. spp. cv. Cobanero from Argentina and Guatemala, respectively as intermediate and C. frutescens cv. Early Spring Giant from China, C. annum cv. Long Green from South Korea, C. spp. cv. NM89C130 and C. pubescens cv. 90002 from Guatemala as sensitive to high temperatures. The tolerant species/genotypes can be used in breeding programs to develop new genotypes that can withstand high temperature conditions both in the present climate and particularly in a future warmer climate. 相似文献
4.
Valérie Verdier Komi Assigbétsé Gopal Khatri-Chhetri Kerstin Wydra Klaus Rudolph Jean-Paul Geiger 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(6):595-602
Strains of Xanthomonas campestris pv. vignicola (Xcv), isolated from cowpea leaves with blight or minute pustules and collected from various geographic areas, were selected on the basis of pathological and physiological features. All strains were analyzed for genotypic markers by two methods: ribotyping with EcoRI endonuclease, and RFLP analysis with a plasmid probe (pthB) containing a gene required for pathogenicity from Xanthomonas campestris pv. manihotis. Ribotyping revealed a unique pattern for all the strains that corresponded to the previously described ribotype rRNA7. Based on polymorphism detected by pthB among Xcv strains, nine haplotypes were defined. The observed genetic variation was independent of the geographic origin of the strains and of pathogenic variation. Some haplotypes were widely distributed, whereas others were localized. In some cases, we could differentiate strains isolated from blight symptoms and pustules according to haplotypic composition. However, in most cases, no significant differences were observed. Our results and the previous pathogenic and biochemical characterizations suggest that the strains isolated from leaves with blight symptoms or minute pustules belong to the same pathovar. We provide information on pathogen diversity that can be used to identify and characterize resistant germplasm. 相似文献
5.
N. Vinod Kumar B. Sreedevi A. Karthik S. Vijaya Lakshmi A. Geetha Reddy D. Sreenivasulu 《Veterinary research communications》2014,38(4):323-328
An outbreak of sheep associated malignant catarrhal fever in crossbred cattle in a village of Andhra Pradesh, southern India, affected thirteen adult cows and two calves from a population of forty animals. All the affected animals were died between December and January 2013–14. The clinical and gross postmortem findings were typical of MCF in Indian crossbred cattle. Migrating sheep flocks were suspected source of infection for the cattle. The diagnosis was confirmed by heminested PCR in all the affected cattle and the suspected sheep flock. The PCR provided evidence of ovine herpes virus type 2. 相似文献
6.
Differential HSP90α expression in fish hepatocytes from polluted estuary during summer 总被引:2,自引:0,他引:2
The molecular chaperone, heat shock protein 90α (HSP90α), plays an important role in protein folding, degradation of denatured
proteins and steroid activation. It is essential for the maintenance of cellular integrity and survival when induced in response
to environmental, physical and chemical stresses. In the present investigation the effect of environmental stress on HSP90α
expression was examined in grey mullet Mugil cephalus living in either a contaminated (Ennore) or uncontaminated (Kovalam) estuary over two seasons: Hepatocytes were isolated
from grey mullet of both estuaries. Oxidative stress was determined along with HSP90α in these fish. Additionally, immunohistochemical
changes were studied to confirm the HSP90α expression. Comparison of the results revealed enhanced hepatocyte oxidative stress
and HSP90α expressions in fish from Ennore to a significant extent than fish from Kovalam. Also, the results showed significant
seasonal variations with maximum expression observed during summer compared to the monsoon season. Overexpression of HSP90α
in hepatocytes exposed to chronic environmental stress by pollutants may confer differential effects on cell survival by protecting
against oxidative stress induced changes. The results also indicate that seasonal variations have significant effect on the
HSP90α expression. 相似文献
7.
Jai Gopal 《Euphytica》1993,72(1-2):133-142
Summary Six hundred and seventy six accessions of cultivated potato (Solanum tuberosum ssp. tuberosum) from 25 countries, were studied for flowering and fruiting behaviour under long days (12–14 h). Flowering intensity ranged from dropping of floral buds just after initiation to profuse blooming. The majority (58.3%) of the accessions bloomed profusely, though 20.4% of the accessions did not bloom at all. Weeks to flowering ranged from 6 to 15 and the majority (66.5%) of the flowering accessions bloomed within 8 to 9 weeks after planting. Duration of flowering ranged from 1 to 10 weeks and the majority (68.1%) of the flowering accessions bloomed for 1 to 4 weeks only. Twentythree per cent of the flowering accessions were completely male sterile. Maximum male fertility was 90% only. No berry setting was observed in 31.8% of the flowering accessions. Only 54.3 per cent of the accessions were found to be fertile in all respects and could be used both as male and female parents. Premature bud abscission was the major cause of sterility. Peru was the best source of profuse-flowering genotypes, Poland was the best source of early flowering genotypes and Mexico was the best source of long duration flowering and good berry setting genotypes. The results suggested that flower bud formation; the growth and development of mature flowers; weeks to flowering and duration of flowering are independent characters controlled by different genes of quantitative nature. Berry setting and duration of flowering were closely associated (r=0.95). Genetic as well as environmental factors interfered with the developmental process leading to flower production and berry setting at different times in different genotypes. The practical implications of these results for true potato seed production are discussed.Publication No. 1298, CPRI, Shimla. 相似文献
8.
The reversible blockage of synthetic pores formed by rigid-rod beta barrels, either by substrates or products, was used to sense a variety of enzymatic reactions in high-throughput format with "naked-eye" fluorescent detection. Improvement of sensor sensitivity beyond three orders of magnitude by straightforward internal mutations underscores the functional plasticity of rigid-rod beta barrels. Such detectors of enzyme activity with the aforementioned characteristics are needed in areas as diverse as proteomics and environmentally benign organic synthesis. 相似文献
9.
Chakrabarty S Das A Bhattacharya A Chakrabarti G 《Journal of agricultural and food chemistry》2011,59(5):2040-2048
Here we studied the antiproliferative activity of theaflavins in cervical carcinoma HeLa cells by investigating their effects on cellular microtubules and purified goat brain tubulin. Theaflavins inhibited proliferation of HeLa cells with IC(50) value of 110 ± 2.1 μg/mL (p = < 0.01), caused cell cycle arrest at G(2)/M phase and induced apoptosis with alteration of expression of pro- and antiapoptotic proteins. Along with these antiproliferative activities, theaflavins act as microtubule depolymerizers. Theaflavins disrupted the microtubule network accompanied by alteration of cellular morphology and also decreased the polymeric tubulin mass of the cells. The polymerization of cold treated depolymerized microtubules in HeLa cells was prevented in the presence of theaflavins. In vitro polymerization of purified tubulin into microtubules was also inhibited by theaflavins with an IC(50) value of 78 ± 2.43 μg/mL (P < 0.01). Thus, disruption of cellular microtubule network of HeLa cells through microtubule depolymerization may be one of the possible mechanisms of antiproliferative activity of theaflavins. 相似文献
10.
Development of Core Subset of Finger Millet Germplasm Using Geographical Origin and Data on 14 Quantitative Traits 总被引:6,自引:0,他引:6
Hari D. Upadhyaya C. L. L. Gowda R. P. S. Pundir V. Gopal Reddy Sube Singh 《Genetic Resources and Crop Evolution》2006,53(4):679-685
Finger millet [Eleusine coracana (L.) Gaertn.] is an important cereal food crop in Africa and South Asia. It is a hardy crop that can be grown in very diverse
environments from almost at sea level to about 2400 m.a.s.l. Finger millet has an excellent food value as its seeds contain
protein ranging from 7 to 14% and are particularly rich in methionine amino acid, iron, and calcium. Despite all these merits,
this crop has been neglected from the main stream of crop improvement research. One of the means to boost its production and
productivity is to enhance utilization of finger millet germplasm to breed superior varieties. Keeping this objective in view,
a core subset of finger millet germplasm (622 accessions) based on origin and data on 14 quantitative traits was developed
from the entire global collection of 5940 accessions held in the genebank at ICRISAT, Patancheru, India. The comparison of
means, variances, frequency distribution, Shannon-Weaver diversity index (H′) and phenotypic correlations indicated that the
core subset represents the entire collection. These tests indicated that sampling was optimal and the diversity has been captured
very well in the core subset. The correlation analysis indicated that panicle exsertion and longest finger length could be
given lower priority in the future germplasm evaluation work of finger millet. 相似文献