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排序方式: 共有156条查询结果,搜索用时 234 毫秒
1.
Jawahar Lal Katara Ram Lakhan Verma Debkanta Nayak Umakanta Ngangkham Soham Ray Hatanath Subudhi Lambodar Behera Sanghamitra Samantaray Ravi Nageswara Rao Onkar Nath Singh Trilochan Mohapatra 《Plant Breeding》2017,136(1):74-82
Identification of new parental lines is crucial for developing ecology‐specific hybrids with ideal agronomic performance. We screened a total of 570 different ecology‐specific Indian rice varieties for the presence of fertility restorer genes, Rf3 and Rf4 using tightly linked markers DRRM Rf3‐10 and RM6100, respectively. Among these varieties, 13% carried Rf3Rf3/Rf4Rf4, 31% carried rf3rf3/rf4rf4, 6% carried Rf3Rf3/rf4rf4 and remaining 50% carried Rf4Rf4/rf3rf3 allelic combinations. A mini set of 40 varieties with variable allelic combinations of fertility restorer genes were testcrossed with WA and Kalinga‐based CMS lines. All the 80 F1s were evaluated for spikelet fertility and fertility restoration ability. Rf3Rf3/rf4rf4 genotypes mostly behaved as partial maintainers or partial restorers. In contrast, rf3rf3/Rf4Rf4 genotypes were partial or effective restorers. However, double dominant genotypes showed better fertility restoration than the genotypes containing Rf3 or Rf4 individually. Some of the genotypes showed unexpected restoration pattern implying occurrence of other fertility restorer(s) apart from Rf3 and Rf4. The perfect restorers and maintainers identified in this study can be directly used in hybrid rice breeding. 相似文献
2.
Identification of internal control genes in milk‐derived mammary epithelial cells during lactation cycle of Indian zebu cow 下载免费PDF全文
3.
Sandeep K. Sharma MVSc Ambika P. Singh MVSc PhD Rishi Tayal MVSc Inder S. Chandna MVSc PhD 《Veterinary radiology & ultrasound》1984,25(1):17-22
Contrast radiography of the alimentary canal was conducted in six adult sheep with barium sulfate (70% W/V, 25–30 ml/kg). A period of 12 hours was adequate for opacification of most parts of the gastrointestinal tract. The presence of contrast medium in different parts of the alimentary canal was noted for as long as 60 hours. The right lateral view was adequate for visualization of most structures, but the typical laminar mucosal pattern of the omasum and the spiral mucosal folds of the abomasum were demonstrated better on the ventrodorsal view. 相似文献
4.
5.
Richa Sood Rekha Khandia Sandeep Bhatia Divakar Hemadri Manoj Kumar Sharan S. Patil Atul K. Pateriya Arshi Siddiqui Malkanna Sanjeev Kumar Mudalagiri Dasappa Venkatesha Diwakar D. Kulkarni 《Tropical animal health and production》2014,46(6):1037-1043
Malignant catarrhal fever (MCF) is a fatal herpesvirus infection of domestic and wild ruminants, with a short and dramatic clinical course characterized primarily by high fever, severe depression, swollen lymph nodes, salivation, diarrhea, dermatitis, neurological disorders, and ocular lesions often leading to blindness. In the present study, fatal clinical cases of sheep associated malignant catarrhal fever (SA-MCF) were identified in cattle in the state of Karnataka. These cases were initially presented with symptoms of diarrhea, respiratory distress, conjunctivitis, and nasal discharges. Laboratory diagnosis confirmed the detection of ovine herpesvirus-2 (OvHV-2) genome in the peripheral blood samples of two ailing animals. The blood samples collected subsequently from sheep of the neighboring areas also showed presence of OvHV-2 genome indicating a nidus of infection in the region. The positive test results were further confirmed by nucleotide sequencing of the OIE approved portion of tegument gene as well as complete ORF8 region of the OvHV-2 genome. Phylogenetic analysis based on the sequence of the latter region indicated close genetic relationship with other OvHV-2 reported elsewhere in the world. 相似文献
6.
Summary Interspecific hybrids have been obtained in an otherwise incompatible cross betweenBrassica juncea × Brassica hirta through the in vitro culture of hybrid ovules and ovaries. The best response was observed from ovules and ovaries cultured 10–15 and 5–7 days after pollination respectively on a basal medium supplemented with indoleacetic acid, kinetin and casein hydrolysate. In some cases the basal cut end of the ovaries proliferated to form callus and shoots. The in vitro-derived hybrid seeds varied in their colour, size and shape, and the F1 plants in the field showed a large diversity in their morphological traits. The hybrids were sterile, and had an intermediate number of chromosomes (2n=30). 相似文献
7.
M. N. Shrivastava Arvind Kumar Sandeep Bhandarkar B. C. Shukla K. C. Agrawal 《Euphytica》2003,130(1):143-145
The Asian rice gall midge, Orseolia oryzae Wood Mason (Diptera: Cecidomyiidae), is a major pest of rice in several South and South East Asian countries. The maggots
feed internally on the growing tips of the tillers and transform them into tubular galls, onion leaf-like structures called
‘silver shoots’ resulting into severe yield loss to the rice crop. We studied the mode of inheritance and allelic relationships
of the resistance genes involved in resistant donor Line 9, a sib of a susceptible cultivar ‘Madhuri’. The segregation behaviour
of F1, F2 and F3 populations of the cross between Line 9 and susceptible cultivar MW10 confirmed the presence of a single dominant gene for
resistance. Tests of allelism with all the known genes giving resistance to this population indicated that Line 9 possessed
a new gene which was designated Gm 9
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
8.
Molecular mapping of a locus controlling resistance to Albugo candida in Indian mustard 总被引:1,自引:0,他引:1
Brassica juncea (L.) Czern & Coss is widely grown as an oilseed crop in the Indian subcontinent. White rust disease caused by Albugo candida (Pers.) Kuntze is a serious disease of this crop causing considerable yield loss every year. The present study was undertaken to identify molecular markers for the locus controlling white rust resistance in a mustard accession, BEC‐144, using a set of 94 recombinant inbred lines (RILs). The screening of individual RILs using an isolate highly virulent on the popular Indian cultivar ‘Varuna’ revealed the presence of a major locus for rust resistance in BEC‐144. Based on screening of 186 decamer primers employing bulked segregant analysis (BSA), 11 random amplified polymorphic DNA markers were identified, which distinguished the parental lines and the bulks. Five of these markers showed linkage with the rust resistance locus. Two markers, OPN0l000 and OPB061000, were linked in coupling and repulsion phases at 9.9 cM and 5.5 cM, respectively, on either side of the locus. The presence of only two double recombinants in a population of 94 RILs suggested that the simultaneous use of both markers would ensure efficient transfer of the target gene in mustard breeding programmes. 相似文献
9.
Suitability of mapped sequence tagged microsatellite site markers for establishing distinctness, uniformity and stability in aromatic rice 总被引:6,自引:0,他引:6
R.K. Singh R.K. Sharma A.K. Singh V.P. Singh N.K. Singh S.P. Tiwari T. Mohapatra 《Euphytica》2004,135(2):135-143
At present, testing for distinctness, uniformity and stability (DUS) of crop varieties relies on a set of morphological characters.
These characters suffer fromthe limitations of number, interaction with the environment in which the variety grows and subjectivity
in decision-making. The potential of DNA-based markers such as sequence tagged microsatellite site (STMS), for establishing
DUS merits investigation. In the present study, a set of 55 mapped STMS markers, selected from 12 linkage groups of rice genome,
was used to examine distinctness of 23 aromatic rice genotypes including the commercially important Basmati varieties. Forty-one
of these markers (74.5%) showed polymorphism between the varieties. The number of alleles per locus ranged from 2–4 with an
average of 2.3. The polymorphism information content (PIC) of the markers varied from 0.083 to 0.665 with an average of 0.338.
All the varieties could be differentiated from each other at a low probability (0.07×10-13) of identical match by chance. The marker-based clustering of the varieties corresponded with the known phenotypic classification,
thereby providing confidence in the distinctness established by the mapped STMS markers. The utility of these markers to study
uniformity and stability was analysed using a commercially important crossbred Basmati rice variety Pusa Basmati 1(IET-10364)
that contributes about 40–50% of Basmati rice export from India. Genotyping of twenty individual plants, grown from the nucleus,
breeder, foundation, certified and farmer's saved seed samples using all the 55 markers revealed no variation among the plants.
These observations suggested that the set of mapped markers employed in this study could be further used for establishing
distinctness of aromatic rice varieties and for studying DUS of the important commercial variety Pusa Basmati 1.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
10.
Sandeep?KumarEmail author Ranjith?P.?Udawatta Stephen?H.?Anderson Ashish?Mudgal 《Agroforestry Systems》2011,83(1):51-62
Buffers have been found to reduce non-point source pollution (NPSP) from watersheds. Hydrologic simulation models assist in
predicting the effects of buffers on runoff and sediment losses from small watersheds. The objective of this study was to
calibrate, validate and simulate runoff and sediment losses and compare buffer effects on NPSP losses relative to control
watersheds (no buffer) for seven years. The experimental design consists of four watersheds under pasture management which
were monitored from 2002 through 2008; two with agroforestry buffers (AgB 100 and AgB 300) and two control watersheds (CW
400 and CW 600). Pasture areas included red clover (Trifolium pretense L.) and lespedeza (Kummerowia stipulacea Maxim.) planted into fescue (Festuca arundinacea Schreb.) while the agroforestry buffer area included Eastern cottonwood trees (Populus deltoids Bortr. ex Marsh.) planted into fescue. The APEX model was calibrated from 2002 to 2005 and was validated from 2006 to 2008.
The r
2
and NSE values for the calibration and validation periods of the runoff varied from 0.52 to 0.78 and 0.50 to 0.74, respectively.
The model did not predict sediment loss very well probably due to insufficient number of measured events and low measured
sediment loss. The measured runoff was 57% higher for CW watersheds compared to AgB watersheds. The measured sediment loss
was 95% higher for CW watersheds compared to AgB watersheds. After calibrating and validating the model, it was run for long-term
scenario analyses for 10 years from 1999 to 2008. Simulated buffer width had a significant influence on runoff. Simulated
runoff decreased by 24% when the buffer width was doubled compared to losses associated with the measured buffer width. Simulated
runoff from the CW watersheds was 11% higher with double stocking density (relative to measured density) compared to AgB watersheds
with double stocking density. With half stocking density (relative to measured density), the AgB watershed had 18% lower runoff
compared to CW. Results from this study imply that establishment of agroforestry buffers on grazed pasture watersheds reduce
runoff and sediment losses compared to control watersheds without buffers. 相似文献