Antibiotic residues in goats milk following intramammary treatment

Two lactational intramammary antibiotic preparations (cloxacillin and oxytetracycline) were each used to treat eight goats by intramammary infusion in one half. The rate of release of the antibiotics was monitored by a diffusion assay based on the sensitive organism Bacillus stearothermophilus var. calidolactis. Cloxacillin was still detectable 156 hours after the final treatment while oxytetracycline was undetectable 108 hours following treatment. A small but significant amount of antibiotic was translocated between treated and untreated halves of the udder in both cases.     

Brucellosis in migratory sheep flock from Maharashtra,India

Brucellosis is a zoonotic disease worldwide distributed and having the economic as well as public health importance. The prevalence of brucellosis among sheep flock having history of abortions was studied. A total of 229 samples comprising of 157 blood and 72 clinical samples (vaginal swabs) were collected from 157 animals. Clinical samples were processed for the isolation of Brucella melitensis. Serum samples (n = 157) were tested by Rose Bengal plate test (RBPT) and i-ELISA. A total of 68 (43.31%) and 104 (66.24%) samples were positive by RBPT and ELISA, respectively. Brucella isolates (n = 2) were recovered from clinical samples. Both isolates demonstrated amplification for bcsp 31 and IS711 genes. On AMOS PCR, both the isolates amplified at 731 bp, i.e., belongs to B. melitensis species. The incidence of B. melitensis in a migratory flock warns the thorough testing and culling of Brucella-infected sheep from the flock on a continuous basis; otherwise, such incidence will be routine and poor farmers will be at a loss.     

Phytosanitary importance of Heterodera glycines for South-east Asian countries

Heterodera glycines is not known to occur in South-east Asian countries except in Indonesia. Specific surveys carried out recently in central India indicate absence of the nematode. The region has a large area under cultivation of soybean, and also other crop and wild hosts. H. glycines causes annual yield losses of about half a million metric tons in 10 major soybean-growing countries. Asia has to import half its demand of soybeans, mainly from North America and Brazil, where H. glycines occurs. Spread is mainly through soil mixed with seeds, or by spillage of grain. A preliminary pest risk analysis suggests that the South-east Asian region is suitable for the introduction and spread of H. glycines . Management practices are suggested to delay its introduction.     

Surveillance and monitoring for plant parasitic nematodes – a challenge

Surveillance of pests is an important activity for (a) early detection and prevention of spread of indigenous and exotic pests, (b) delimiting pest distribution, (c) searching for pest free areas for meeting the requirements of national and international trade and (d) to achieve effective management of pests that affect crop production. Recent developments on these topics are reviewed with special reference to plant parasitic nematodes in India.     

Lagenidium callinectes (Couch, 1942) infection and its control in cultured larval Indian tiger prawn, Penaeus monodon Fabricius

A mycosis in larval tiger prawn, Penaeus monodon Fabricius, is described for the first time from India. The hyphae of Lagenidium callinectes are contorted, irregularly branched, sparingly septate, and contain a cell wall and membrane, vacuoles, mito chondria, ribosomes, small and large vesicles, and Woronin bodies. The spores occur singly or in pairs. The fungal mycelium may either invade and embed itself in the tissues, or alternatively, replace all the muscle tissues of the infected larval P. monodon. Fungus infected, untreated populations of nauplii, zoea and myksis exhibited mortalities of 5.33 ± 0.55%, 24.68 ± 4.58% and 47.89 ± 0.27%, respectively. A 0.5 ppm treatment with trifluralin significantly reduced the mortality of infected larval populations (i.e. 1.1% nauplii, 3.28% zoea and 5.21% myksis mortality). Lagenidium sp. exhibited growth in potato dextrose agar medium and in Sabouraud's agar at 28 °C.     

Identification of a diverse mini‐core panel of Indian rice germplasm based on genotyping using microsatellite markers

Identification of a small core germplasm set representing the available genetic diversity is essential for its proper evaluation and subsequent utilization in rice improvement programmes. For constituting a small diverse mini‐core panel of Indian rice germplasm, a representative set of 6912 accessions drawn based on their geographic origin from the whole rice germplasm collection available in the National Gene Bank was genotyped using 36 microsatellite markers. Automated fragment analysis of amplicons yielded a total of 435 alleles, with an average 12.4 and range of 3–29 alleles per locus. Polymorphism information content (PIC) ranged from 0.08 (RGNMS190) to 0.86 (RM552) with an average of 0.528. Based on genotyping data, a mini‐core consisting of 98 genotypes was identified. Ninety‐four per cent of the alleles present in the core set were present in the mini‐core. The identified small but diverse panel will be useful for further intensive trait‐specific evaluation and utilization in allele mining.     

Effect of tillage and mode of straw mulch application on soil erosion in the submontaneous tract of Punjab, India

The submontaneous tract of Punjab comprising 10% of the state, is prone to soil erosion by water. Soils of the area are coarse in texture, low in organic matter and poor in fertility. High intensity rains during the monsoon season result in fertile topsoil removal. There is an urgent need to control soil erosion in this region so as to improve soil productivity. A field study was conducted to estimate the effect of tillage and different modes of mulch application on soil erosion losses. Treatments comprised two levels of tillage, viz. minimum (T_m) and conventional (T_c) in the main plots and five modes of straw mulch application, viz. mulch spread over whole plot (M_w), mulch spread on lower one-third of plot (M_1/3), mulch applied in strips (M_s), vertical mulching (M_v) and unmulched control (M_o), in subplots in a replicated split plot design. Rate of mulch application was 6 t ha⁻¹ in all modes. Compared with M_o, M_w reduced runoff by 33%. Runoff and soil loss were 5 and 40% higher under T_c than under T_m. Though other modes of straw mulch application (M_1/3, M_s and M_v) controlled soil loss better than M_o, their effectiveness was less than M_w. T_m was more effective in conserving soil moisture than T_c. Compared with M_o, M_w had 3–7% higher soil moisture content in the 0–30 cm soil depth under T_m. Minimum soil temperature of the surface layer was 1.4–2.4 °C lower under M_w than under M_o. Straw mulching reduced maximum soil temperature and helped in conserving soil moisture. Minimum tillage coupled with M_w was highly effective in reducing soil erosion losses, decreasing soil temperature and increasing moisture content by providing maximum surface cover.     

Tracks of cosmic rays in plastics

Cosmic ray nuclei have been observed with the use of plastic trackdetecting solids in satellites and high-altitude balloon flights. Nuclear emulsions in the stacks of plastic sheets allowed the positive identification of cosmic raynuclei as light as nitrogen. The most striking new information was the failure to observe relativistic iron nuclei, a result which has led to an advance in the understanding of track registration criteria.     

Micrometeorite craters discovered on chondrule-like objects from kapoeta meteorite

Craters attributable to hypervelocity impacts of micrometeorites have been discovered on rare chondrule-like objects from the gas-rich meteorite Kapoeta. These chondrule-like objects, probably generated by impacts themselves, provide further evidence for the regolith origin of Kapoeta. The micrometeorite flux at the time of formation of the meteorites was probably an order of magnitude higher than the present flux, but the solar luminosity could not have been higher than 1.7 times its present value.     

Genetic Diversity for Morphological and Quality Traits in Quinoa(<Emphasis Type="Italic">Chenopodium quinoa</Emphasis> Willd.) Germplasm

Twenty nine germplasm lines of Chenopodium quinoa and two of Chenopodium berlandieri subsp. nuttalliae were evaluated for 12 morphological and 7 quality traits for two test seasons. The 19 traits were analyzed for cluster and principal component analysis. The first four PCs contributed 78.70 % of the variability among the germplasm lines. The first PC accounted for 39.5% of the variation and had inflorescence/plant, plant height and stem diameter as the traits with largest coefficients, all with positive sign. The characters with greatest positive weight on PC₂ were days to maturity (0.309), inflorescence length (0.260) and branches/plant. All the germplasm lines were grouped into six clusters based on average linkage method. Cluster III had high values for seed yield and most of the quality traits but showed a small seed size. The dendrogram separated the two lines of C. berlandieri subsp. nuttalliae from the quinoa lines.