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1.
T. Matsumoto Y. Nagashima K. Takayama K. Shimakura K. Shiomi 《Fish physiology and biochemistry》2005,31(1):95-100
In vitro accumulation of tetrodotoxin (TTX) and paralytic shellfish toxin (PST) in tiger puffer fish Takifugu rubripes was investigated using liver tissue slices. When T. rubripes liver slices were incubated with Leibovitz’s L-15 medium containing 0.13 mM TTX at 20 °C in air with saturated humidity, they accumulated 21.5 ± 7.3 μg TTX g?1 liver after the incubation for 12 h and increased to 55.3 ± 8.2 μg TTX g?1 liver at 48 h. In the incubation of T. rubripes liver slices with 0.13 mM PST-containing medium, PST was detected 6.3 ± 0.9 μg g?1 liver at 12 h and reached a plateau thereafter. These results reveal the difference between TTX and PST in accumulation in T. rubripes liver tissue slices. To examine the variation in PST accumulation among fish species, the liver tissue slices from tiger puffer fish T. rubripes, parrot-bass Oplegnathus fasciatus and green ling Hexagrammos otakii were incubated at a concentration of 0.027 mM PST. The toxin contents of 3.0 μg g?1 liver were observed at 8 h regardless of fish species but were not increased subsequently, showing no variety among these three species as to accumulation patterns of PST. It is noted that the tiger puffer fish T. rubripes liver specifically accumulate TTX in preference to PST. 相似文献
2.
A protein that binds to a cis-acting element of wheat histone genes has a leucine zipper motif 总被引:34,自引:0,他引:34
T Tabata H Takase S Takayama K Mikami A Nakatsuka T Kawata T Nakayama M Iwabuchi 《Science (New York, N.Y.)》1989,245(4921):965-967
3.
Takayama H Shimada N Mikami O Murata H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(5):531-533
We evaluated the immunotoxicity of deoxynivalenol (DON), a Fusarium mycotoxin, on bovine and porcine neutrophils in vitro by using two function parameters, luminol-dependent chemiluminescence and random migration under agarose. A 2-hr DON treatment suppressed the chemiluminescence of bovine and porcine cells by 42% and 35% (on average) at 10(-5)M, and by 19% and 26% at 10(-6)M. Slight suppression was observed at concentrations lower than 10(-6)M. However, after an 18-hr DON treatment, random migration of neutrophils of both species remained unaffected, even at the highest concentration (10(-5)M). Although further extensive studies are needed, to our knowledge this is the first study to have revealed in vitro that DON can affect neutrophil function. 相似文献
4.
We determined the chromosome number of 52 accessions of Paspalum from the germplasm collection of the Centro de Pesquisa de
Pecuária do Sudeste (CPPSE/EMBRAPA) originating from different regions in Brazil. All accessions belonged to the Plicatula
group, 13 of them being P. plicatulum, three P. guenoarum, two P. yaguaronense, two P. compressifolium, one P. atratum, and
31 still unidentified Paspalum sp. Except for a P. yaguaronense accession that presented 2n=60, all the remaining ones presented
2n=40 chromosomes. This is the first report of the chromosome number of P. atratum. Considering that the basic number for
most species in the genus Paspalum is ×=10, the accessions analyzed are tetraploids (2n=4×=40) and hexaploids (2n=6×=60).
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
5.
Fisheries Science - The present study established an age determination technique and analyzed growth patterns in three sculpins, Myoxocephalus jaok, Enophrys diceraus, and Gymnocanthus... 相似文献
6.
Takayama I Kubo M Takenaka A Fujita K Sugiyama T Arai T Yoneda M Sato H Yanai T Kai C 《Comparative immunology, microbiology and infectious diseases》2009,32(6):539-549
Ten wild masked palm civets infected with canine distemper virus (CDV), captured in Japan from 2005 to 2007, were histopathologically and phylogenetically analyzed. Phylogenetic analysis based on the amino acid sequences of the H protein of two CDV isolates from masked palm civets revealed that the two isolates were classified into the clade of recent isolates in Japan. Histopathologically marked lesions of virus encephalitis were present in the brain, whereas gastrointestinal lesions were absent or at a mild degree. The distribution of the lesions resembles that of recent CDV cases in dogs. Therefore, recent CDV infections in masked palm civets could be caused by recently prevalent CDV in dogs. The possibility of the masked palm civet as a spreader of CDV among wildlife is also discussed. 相似文献
7.
8.
Kubo K Entani T Takara A Wang N Fields AM Hua Z Toyoda M Kawashima S Ando T Isogai A Kao TH Takayama S 《Science (New York, N.Y.)》2010,330(6005):796-799
Self-incompatibility in flowering plants prevents inbreeding and promotes outcrossing to generate genetic diversity. In Solanaceae, a multiallelic gene, S-locus F-box (SLF), was previously shown to encode the pollen determinant in self-incompatibility. It was postulated that an SLF allelic product specifically detoxifies its non-self S-ribonucleases (S-RNases), allelic products of the pistil determinant, inside pollen tubes via the ubiquitin-26S-proteasome system, thereby allowing compatible pollinations. However, it remained puzzling how SLF, with much lower allelic sequence diversity than S-RNase, might have the capacity to recognize a large repertoire of non-self S-RNases. We used in vivo functional assays and protein interaction assays to show that in Petunia, at least three types of divergent SLF proteins function as the pollen determinant, each recognizing a subset of non-self S-RNases. Our findings reveal a collaborative non-self recognition system in plants. 相似文献
9.
Akiho KATAYAMA Ayako MIYAZAKI Naohito OKAZAKI Teruko NAKAYAMA Osamu MIKAMI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(6):931
A total of ten 1–2-year-old rabbits died within 2 weeks at a facility in Ehime prefecture in May 2019. Necropsy revealed liver discoloration and fragility, hemorrhage of some organs and blood coagulation failure. On histopathologic examination, necrotizing hepatitis was a common finding, together with fibrin thrombi in the small vessels and hemorrhage in some organs. Rabbit hemorrhagic disease (RHD) virus gene was detected in liver samples, and viral particles of approximately 32 nm in diameter were found in the cytoplasm of degenerated hepatocytes by electron microscopy. Phylogenetic analysis based on the partial VP60 gene sequence classified it as Lagovirus europaeus GI.2/RHDV2. This is the first confirmed outbreak of RHD caused by globally emerging GI.2/RHDV2 in Japan. 相似文献
10.
Tadashi Oikawa Toshiya Matsui Yasunori Matsuda Teruko Takayama Hitoshi Niinuma Yasuyo Nishida Kazuo Hoshi Mitsuyoshi Yatagai 《Journal of Wood Science》2006,52(2):140-146
Volatile organic compounds (VOCs) from wood and their effects on artifacts are of intense interest to museum staff who must
strictly manage the preservation environment. In this study, the causal substances of deterioration were inferred by comparison
with laser Raman (LR) spectra of deterioration products. The artifact samples were deteriorated using VOCs from woods (WV
test), and using specific substances that occur in wood VOCs (SV test). The deteriorated samples were analyzed by LR spectroscopy
and the results of the WV and SV tests were compared.
Hinokitiol and acetic acid were found to be two of the main causal substances of deterioration in western red cedar. Deterioration
of iron and copper by spruce did not appear to be caused by acetic acid, as is generally assumed, but by some other unknown
compound(s). Sensitivity to wood-based VOC components depended on the type of artifact. In western red cedar, mitsudasou (litharge) was very sensitive to acetic acid, while other artifacts were sensitive to hinokitiol. The LR method used in this
study is very useful for the inference of causal substances of deterioration based on the detection of small amounts of deteriorated
products such as those generated by deterioration due to wood VOCs.
Part of this article was presented at the 25th Annual Meeting of the Japan Society for the Conservation of Cultural Property,
Kyoto, June 2003 相似文献