DUBA: a deubiquitinase that regulates type I interferon production

Production of type I interferon (IFN-I) is a critical host defense triggered by pattern-recognition receptors (PRRs) of the innate immune system. Deubiquitinating enzyme A (DUBA), an ovarian tumor domain-containing deubiquitinating enzyme, was discovered in a small interfering RNA-based screen as a regulator of IFN-I production. Reduction of DUBA augmented the PRR-induced IFN-I response, whereas ectopic expression of DUBA had the converse effect. DUBA bound tumor necrosis factor receptor-associated factor 3 (TRAF3), an adaptor protein essential for the IFN-I response. TRAF3 is an E3 ubiquitin ligase that preferentially assembled lysine-63-linked polyubiquitin chains. DUBA selectively cleaved the lysine-63-linked polyubiquitin chains on TRAF3, resulting in its dissociation from the downstream signaling complex containing TANK-binding kinase 1. A discrete ubiquitin interaction motif within DUBA was required for efficient deubiquitination of TRAF3 and optimal suppression of IFN-I. Our data identify DUBA as a negative regulator of innate immune responses.     

Diversity of ectomycorrhizal fungus assemblages on nursery grown European beech seedlings

? Context

An important feature of forest nursery management is the production of first-rate planting stock, which is closely connected with an adequate development of ectomycorrhizas on seedling roots.

? Aims

The aim of this study was to provide a detailed assessment of the ectomycorrhizal (ECM) fungal diversity of European beech grown in bare-root forest nursery conditions.

? Methods

Morphological and molecular analyzes were used to identify ECM fungi, quantify fungal richness, and record differences in the relative abundance of individual taxa.

? Results

Twenty-seven different mycorrhizal fungal taxa were found in four tested nurseries. Of these 27, only 6 were present in more than one nursery. Taxa richness in individual nurseries ranged from 6 to 13, indicating that ECM colonization potential is nursery-dependent. The most frequent taxa were: Tuber sp. 1, Cadophora sp., Cenococcum geophilum agg., Hebeloma sacchariolens, and Laccaria tortilis. According to the analysis of similarity, ECM fungal communities were significantly different between nurseries (R?=?0.7741; p?<?0.0001).

? Conclusion

Beech seedlings grown in bare-root nurseries are colonized by a highly diverse suite of ECM fungi; thus, artificial inoculation of seedlings is generally not necessary in nursery practice.     

Effect of methanabol (17 beta hydroxy-17 alpha methyl-1,4-androstadiene-3-one) on growth and breast muscle nucleic acid content in the chicken

1. The effect of methanabol (17 beta hydroxy-17 alpha methyl-1,4-androstadiene-3-one) on growth and breast muscle nucleic acid in White Rock chickens grown to 8 weeks of age was tested. Methanabol was administered by mouth daily during this period at 0.05 mg/kg body weight in aqueous suspension to 30 birds, with appropriate controls. 2. Growth was significantly increased by methanabol. 3. Breast muscle RNA was significantly increased by methanabol but DNA was not affected.     

Past,present and future suitable areas for the relict tree Pterocarya fraxinifolia (Juglandaceae): Integrating fossil records,niche modeling,and phylogeography for conservation

European Journal of Forest Research - Pterocarya fraxinifolia, native to the southern Caucasus and adjacent areas, has been widely introduced in Europe. In this study, we investigate the following:...