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1.
M. E. Nairn M.Sc. Ph.D. A. R. A. Watson M.R.C.V.S.† 《Australian veterinary journal》1972,48(12):645-656
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Regulation of chloride channels by protein kinase C in normal and cystic fibrosis airway epithelia 总被引:21,自引:0,他引:21
M Li J D McCann M P Anderson J P Clancy C M Liedtke A C Nairn P Greengard M J Welsch 《Science (New York, N.Y.)》1989,244(4910):1353-1356
Apical membrane chloride channels control chloride secretion by airway epithelial cells. Defective regulation of these channels is a prominent characteristic of cystic fibrosis. In normal intact cells, activation of protein kinase C (PKC) by phorbol ester either stimulated or inhibited chloride secretion, depending on the physiological status of the cell. In cell-free membrane patches, PKC also had a dual effect: at a high calcium concentration, PKC inactivated chloride channels; at a low calcium concentration, PKC activated chloride channels. In cystic fibrosis cells, PKC-dependent channel inactivation was normal, but activation was defective. Thus it appears that PKC phosphorylates and regulates two different sites on the channel or on an associated membrane protein, one of which is defective in cystic fibrosis. 相似文献
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SUMMARY Palpable lesions within the scrotum but not associated with the testis or epididymis were detected in 20 of 200 merino rams in 3 flocks during routine veterinary examination. In 13 of 15 rams further examined Corynebacterium pseudotuberculosis was isolated from the lesions, which were typical CLA type abscesses. C. pseudotuberculosis was not shed in semen, and semen quality was normal in all affected rams. 相似文献
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M E Nairn 《Avian diseases》1973,17(3):504-517
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Hayley R. Tumas Brian M. Shamblin Mark Woodrey Nathan P. Nibbelink Richard Chandler Campbell Nairn 《Landscape Ecology》2018,33(9):1585-1601
Context
Common species important for ecosystem restoration stand to lose as much genetic diversity from anthropogenic habitat fragmentation and climate change as rare species, but are rarely studied. Salt marshes, valuable ecosystems in widespread decline due to human development, are dominated by the foundational plant species black needlerush (Juncus roemerianus Scheele) in the northeastern Gulf of Mexico.Objectives
We assessed genetic patterns in J. roemerianus by measuring genetic and genotypic diversity, and characterizing population structure. We examined population connectivity by delineating possible dispersal corridors, and identified landscape factors influencing population connectivity.Methods
A panel of 19 microsatellite markers was used to genotype 576 samples from ten sites across the northeastern Gulf of Mexico from the Grand Bay National Estuarine Research Reserve (NERR) to the Apalachicola NERR. Genetic distances (FST and Dch) were used in a least cost transect analysis (LCTA) within a hierarchical model selection framework.Results
Genetic and genotypic diversity results were higher than expected based on life history literature, and samples structured into two large, admixed genetic clusters across the study area, indicating sexual reproduction may not be as rare as predicted in this clonal macrophyte. Digitized coastal transects buffered by 500 m may represent possible dispersal corridors, and developed land may significantly impede population connectivity in J. roemerianus.Conclusions
Results have important implications for coastal restoration and management that seek to preserve adaptive potential by sustaining natural levels of genetic diversity and conserving population connectivity. Our methodology could be applied to other common, widespread and understudied species.8.
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