Mitochondrial DNA analysis of Nepalese domestic dwarf cattle Lulu*

Dwarf Lulu cattle, the only Bos Taurus type of cattle in Nepal, are raised under severe environments in the mountainous zone of that country. In the present study, the body measurement traits, cytogenetic and molecular genetic characteristics of the Lulu cattle are investigated. Blood samples were collected from 31 animals in four villages (altitudes 2590–3550 m) in the southern part of Mustang. The Lulu cattle had a normal karyotype with 2n = 60, XY or XX. Only one male examined had a large submetacentric X‐chromosome and a small submetacentric taurine type Y‐chromosome. The mitochodrial DNA (mtDNA) genotypes were analyzed by PCR mediated restriction fragment length polymorphisms, displacement (D)‐loop region PCR mediated single strand conformation polymorphisms, and D‐loop region sequences. Many base substitutions were found in the D‐loop region, suggesting that the Lulu cattle originated from at least 10 maternal lines. Three types of mtDNA from these cattle were found, the Bos taurus type (n = 23), the Bos indicus type (n = 6), and the Bos grunniens type (n = 2). In the village at the lowest altitude, four of the five cows were of the Bos indicus type. These results indicated that mtDNA types of the Lulu cattle mostly belong to Bos taurus, but have been hybridized with Bos indicus cattle in lower‐elevation regions in their maternal lineage.     

Impact of agroforestry intervention on farm income under the subsistence farming system of the middle hills, Nepal

Practices that minimize the rate of soil degradation, increase crop yields and raise farm income are key to sustaining agricultural productivity in the hills of Nepal. The use of farmland is undergoing rapid changes in response to increasing population pressure, deforestation and subsistence needs. Against this background, this study examined the impact of an agroforestry intervention project on farm income based on a sample of subsistence farm households in Dhadhing district. The project was implemented by Nepal Agroforestry Foundation in 1993/94 to increase fodder production through the promotion of agroforestry. A total of 223 households (82 with project and 141 without project) were interviewed during May–October 1998 to collect information on production and agroforestrys' impact on farm income. The benefit-cost analysis showed that the agricultural system including agroforestry was more profitable than the conventional one. The results also showed that the introduction of mulberry trees for sericulture could further enhance the profitability of an agroforestry-based system. Thus, agroforestry has great potential for enhancing food production and farmers' economic conditions in a sustainable manner through its positive contributions to household income.This revised version was published online in November 2005 with corrections to the Cover Date.     

Influence of cyto‐sterility sources of female line on seed quality of Indian mustard (Brassica juncea L. Czern & Coss.) in relation to storage period

Effect of cyto‐sterility sources on morphology and yield attributes in F₁ has well been studied in different crops, but not on the seed quality of hybrid. Six cytoplasmic male sterile (CMS) lines, viz. Ogura, Siifolia, Erucoides, Moricandia, Tournefortii and Oxyrrhina, which represent six different sources of CMS, were pollinated by a single maintainer line (‘Pusa Bold’) to check whether CMS sources have any marked effect on seed quality. Seeds were collected in 2007–2008 (rabi season: October–April) from CMS maintenance plot and kept in ambient storage for next 3 years. The results indicated that CMS/cyto‐sterility sources influenced the seed quality parameters in fresh seed as well as after storage. Per cent germination (as means over the storage period) was recorded up to 89.00, 88.25 and 87.88 in Moricandia, Ogura and Oxyrrhina systems, respectively, and it was significantly different from Tournefortii and ‘Pusa Bold’ (70.13 and 74.38, respectively). The CMS sources also had pronounced effect on other seed quality traits, viz. root and shoot length and seedling dry matter, and Ogura and Moricandia (green) performed better.     

Participatory urban forestry in Nepal: Gaps and ways forward

Managing urban population and environment has been one of the most important challenges recently. This paper attempts to assess the existing practices, people's perception, gaps and ways forward for participatory urban forestry and aims to help promote UF through identifying the current status, future prospects for and peoples’ perception about UF in two cities of Nepal. The study was conducted in Pokhara sub-metropolitan city and Bharatpur municipality of Nepal. Total numbers of plants in the effective green spaces are estimated as 285,500 in Pokhara and 213,250 in Bharatpur, and the number of tree species was 62 and 52 respectively. The religious tree species – Ficus bengalensis and Ficus religiosa are the most common and hazardous in both cities. The urban people have preferred small, less branchy and attractive tree species. However, the choice of existing species is inappropriate and not according to the people's interest. Therefore, urban people are unsatisfied with the existing species and their frequencies. The municipal authorities and other related organizations do not have any specific plan, programs and activities nor is there any public involvement to promote urban forestry. Regular coordination, cooperation and monitoring are required for the involvement of various stakeholders such as municipality, government office, community organizations and urban people. Analysis further shows that people's perception on urban forestry is encouraging as most of them agreed that the urban forests are useful for addressing the challenging urban environment. Adequate institutions and financial resources coupled with effective cooperation and coordination among concerned organizations could ameliorate the urban environment as urban community forestry.     

The implications and challenges of First Nations forestry negotiations in British Columbia,Canada: The Tl’azt’en Nation experience

Abstract

This article examines the implications and limitations of First Nations forestry negotiations in British Columbia (BC), Canada by assessing how effectively the Tl’azt’en Nation is able to realize benefits under the current form of negotiations. The primary goal of the Tl’azt’en Nation has always been to defend their Aboriginal interests within their ancestral lands, which would entail the cessation of clear-cutting on their traditional territory. Although the community has pursued some economic opportunities in the forestry sector to improve their socioeconomic conditions, despite their efforts, the socioeconomic status of the community remains unchanged. The collective experience of the Tl’azt’en Nation illustrates that simply obtaining certain forest tenures and short-term-based revenue sharing agreements does not adequately meet their forestry and self-sufficiency goals. Without addressing the fundamental issues and challenges facing the community, the current practice of forestry negotiations between the Provincial government and the Tl’azt’en Nation has produced no significant benefits to the community.     

Factors determining the adoption of laser land leveling in the irrigated rice–wheat system in Haryana,India

Laser land leveling has been increasingly adopted in the irrigated rice (Oryza sativa L.)–wheat (Triticum aestivum L.) cropping system in the state of Haryana (India), located in the north-western Indo-Gangetic Plains. Still, many farmers have applied it to only a fraction of their land. In this study, we used data collected from 621 farm households in Haryana and applied a double-hurdle model for assessing the factors that determine the adoption and intensity of laser-leveling technology. The results show that large land holders are more likely to laser level their farm land; however, we found a negative association between land holdings and the proportion of laser-leveled land. Information about technology through farmer-to-farmer communication and through private traders, participation in agricultural training and membership in local agricultural institutions increased both the likelihood and the intensity of adoption. Our findings call for a closer collaboration among the various stakeholders, specifically to promote farmer-to-farmer communication through increased participation in local institutions and increase the rate of adoption of laser leveling technology.     

Marker-assisted selection of low erucic acid quantity in short duration Brassica rapa

Low erucic acid (LEA) rapeseed, which has accumulated mutant fatty acid elongase genes at the BnFAE1.1 and BnFAE1.2 loci of the A- and C-genome, respectively, is an important oilseed crop. Short growing turnip rape (B. rapa) is also important as a catch crop in the continuous cropping of rice in Asia but there is no LEA B. rapa cultivar for cultivation in South Asia. In order to develop LEA turnip rape cultivars, high erucic acid turnip rape cultivars were interspecifically crossed as recurrent parents to a canola quality rapeseed. In the meantime, we monitored incorporation of the mutant bnfae1.1 (e1) gene into A-genome of turnip rape, using a dCAPS primer pair, which can amplify PCR fragment only for the mutant e1 gene from A-genome. The early backcross progenies showed poor seed set, but which was improved in advanced progenies. Finally, homozygous e1e1 genotypes were established in the selfed progenies of BC₂–BC₃, and their LEA content was confirmed by gas-chromatography analysis. Our results and promising lines will contribute to LEA-trait selection in turnip rape and rapeseed breeding.     

Can We Reduce Rainfed Maize (Zea mays L.) Nitrogenous Fertilizer Application Rate with Addition of Nitrapyrin?

Application of nitrification inhibitor has potential to increase soil nitrogen (N) retention throughout the growing season and finally increase corn (Zea mays L.) yield. During the 2012–2014 growing seasons, on-farm field trials were conducted to determine the effects of nitrapyrin (Instinct) with two N sources, urea and urea ammonium nitrate, at two rates, 85% and 100% of recommended N, and side-dress on grain yield and soil inorganic N availability in the Red River Valley of the North Dakota. Preplant urea N at 100% recorded the greatest yield in 2 out of 3 years. At late sampling, the greatest soil inorganic N was observed with side-dress urea ammonium nitrate at 100% within 0–30 cm (last 2 years). For spring fertilizer N management, addition of nitrapyrin had no effect on yield and inconsistent effect on soil N availability. Our results suggest that fertilizer N management should be evaluated on a local scale and consider annual variability in weather.     

Mitigating Negative Microbial Effects of <Emphasis Type="Italic">p</Emphasis>-Nitrophenol,Phenol, Copper and Cadmium in a Sandy Loam Soil Using Biochar

Biochars are adsorptive solids potentially of benefit to soil microbes by providing improved nutrient retention, a carbon substrate and contaminant adsorption. A 28-day incubation experiment gauged the interactive effects of biochar application and contaminants on the microbial biomass and respiration of a sandy loam soil. Soil was amended with 250 mg/kg phenol or p-nitrophenol (two toxic but nevertheless biodegradable organic contaminants) or 50 mg/kg cadmium or copper. Biochar application generally caused increased microbial respiration and biomass relative to non-amended controls. Of the heavy metal-amended soils, Cu effected significant reductions in microbial biomass carbon and basal respiration, which were improved with concurrent biochar amendment. The biochar’s functional groups are likely to have mitigated the metals’ negative effects via complexation and sorption, while the soil’s proportion of negative pH-dependent sites was increased by the pH rise induced by biochar application, allowing more cationic retention. Organic contaminant-spiked soils had higher microbial biomass-specific respiration without biochar amendment, indicating that surviving microbes utilised the compounds and necromass as substrates. Paranitrophenol proved to be particularly toxic without biochar application, causing marked reductions in the microbial quotient and biomass carbon. Remarkably, concurrent biochar and pNP application led to hugely increased microbial biomass carbon and nitrogen, significantly higher than those in contaminant-free replicates. It is likely this arose from biochar sorbing the contaminant and allowing its microbial utilisation as a carbon and nitrogen source, stimulating growth. Biochar application is a highly promising strategy for reducing the soil microbial toxicity of heavy metals and aromatic organic contaminants, particularly p-nitrophenol.