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排序方式: 共有141条查询结果,搜索用时 15 毫秒
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Lien VANDE MAELE Marc HEYNDRICKX Dominiek MAES Nele DE PAUW Maxime MAHU Marc VERLINDEN Freddy HAESEBROUCK An MARTEL Frank PASMANS Filip BOYEN 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):325-328
The antibacterial potential of organic acids and essential oil components against Brachyspira
hyodysenteriae, the causative pathogen of swine dysentery, was evaluated. Minimum inhibitory
concentrations (MIC) of 15 compounds were determined at pH 7.2 and pH 6.0, using a broth microdilution assay.
In addition, possible synergism was determined. MIC values for the three tested strains were similar. For
organic acids, MIC values at pH 6.0 were lower than at pH 7.2. B. hyodysenteriae was most
sensitive to cinnamaldehyde and lauric acid, with MIC values <1.5 mM. Most antibacterial effects of binary
combinations were additive, however, for thymol and carvacrol, synergism could be observed. In
vitro results demonstrate the antibacterial action of certain essential oil components and organic
acids against B. hyodysenteriae. 相似文献
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过去人们常认为,由于刚出壳雏鸡的免疫系统尚未发育成熟,同时肠道内缺乏有益的微生物,因而处于高度的敏感期,此时接种沙门氏菌苗产生的保护作用很有限。然而,最新的研究表明,在这些敏感期接种疫苗,可诱发产生保护作用。 相似文献
5.
In well-aerated culture solutions Ca-montmorillonite at 0.25% concentration markedly accelerated and increased growth, glucose consumption and CO2 evolution by various Streptomyces, Micromonospora and Nocardia species. The montmorillonite was a little more active than Ca-humate and was usually still but somewhat less effective when confined to dialysis tubing. Ca-exchange resin, Na2SiO3 and finely powdered CaCO3 exerted very little or no effect. In many cultures the relation of glucose consumption to biomass formation indicated a more efficient use of the glucose C for cell synthesis in the presence of clay. In other cultures the greater biomass formation was associated with a more rapid and complete utilization of the glucose present. 相似文献
6.
Seven types of fungal melanins were compared with humic compounds from chernozem and podzol soils and with commercial humic acid. The results indicate some similarities but also distinct differences between the melanins and humic substances in spectral characteristics in the UV and visible regions and in their resistance to thermal degradation. 相似文献
7.
Franklin Prez Filip A.M. Volckaert Jorge Caldern 《Aquaculture (Amsterdam, Netherlands)》2005,250(3-4):586-591
White Spot Syndrome Virus (WSSV) has decimated the shrimp aquaculture around the world. Breeding efforts to generate resistant stocks are necessary but there is a lack of basic information on challenge test strategies focused on genetic selection. Infection routes and developmental stages were evaluated on Penaeus vannamei as a first step in a strategy to select white spot virus (WSSV)-resistant stocks. Mortalities could not be induced before the PL30 stage. The impact of infection by immersion and blended tissue was intermediate on mortalities when compared to the minced tissue treatment on PL30. Blended and minced tissue treatments produced the highest mortalities on PL40 while immersion was intermediate. A general tendency towards higher susceptibility associated with older stages was detected. Additionally, juveniles of 1 g average weight from three local breeding programs were challenged. There were no differences in survival between the programs, although two of them derived their progenies from survivors of strong WSSV events. The implications of these results to the WSSV epidemiological characteristics and breeding programs are discussed. 相似文献
8.
Eighteen chickens were immunized subcutaneously with purified type 1 fimbriae from Salmonella enterica serotype Enteritidis at 18 and 21 weeks of age. Evidence of IgG and IgA responses was found in the eggs and in the sera of the immunized hens. Three weeks later, immunized and non-immunized chickens (n=18) were challenged intravenously with 2x10(7) live Salmonella enterica serotype Enteritidis. There was no significant difference in the numbers of eggs laid by immunized and non-immunized birds. The percentage of Salmonella contaminated eggs was significantly higher in the non-immunized group than in the immunized group due to a higher percentage of contamination of the externally disinfected egg shells. There were no statistical differences in the percentages of contaminated yolks and egg whites between control and immunized birds. No differences in the number of colonizing bacteria could be found in the spleen nor in the liver between the immunized and the control groups throughout the experiment. Salmonella was cleared from the ovary of the immunized birds in the second week p.i., in contrast to the control birds where Salmonella was isolated till the third week after infection. Oviducts were significantly more infected in the control group than in the immunized group. Salmonella was cleared from the oviducts at 3 weeks p.i. in the immunized hens but not in the control hens. In conclusion, we demonstrated that the immunization of laying hens with type 1 fimbriae reduced the number of contaminated eggs and reduced the colonization of the reproductive organs. 相似文献
9.
Molecular and phenotypical characterization of Clostridium perfringens isolates from poultry flocks with different disease status 总被引:1,自引:0,他引:1
Gholamiandekhordi AR Ducatelle R Heyndrickx M Haesebrouck F Van Immerseel F 《Veterinary microbiology》2006,113(1-2):143-152
Due to the diminished use of growth-promoting antibiotics in the European Union, Clostridium perfringens induced necrotic enteritis and subclinical disease have become important threats to poultry health. A study was set up to genotypically and phenotypically characterise C. perfringens isolates from poultry flocks with different health status. Animals from healthy flocks were sampled by cloacal swabs, while intestinal and liver samples of animals suffering from necrotic enteritis were analysed. A total of 27 isolates was obtained from 23 broiler flocks without clinical problems and 36 isolates were obtained from 8 flocks with clinical problems. Using PFGE typing, high genetic diversity was detected between isolates from different flocks. Isolates derived from flocks where disease outbreaks occurred were clonal within each flock, but each flock harboured a different clone. All isolates were of toxin type A. Isolates from 5 out of 35 PFGE types carried the cpb2 gene, encoding the beta2 toxin, and isolates from 2 out of 35 PFGE types harboured the cpe gene, encoding the enterotoxin. In vitro alpha toxin production for all isolates was quantified by enzyme-linked immunosorbent assay. It was shown that in vitro alpha toxin production of C. perfringens isolates from diseased flocks was not higher than in vitro alpha toxin production from isolates derived from healthy flocks. 相似文献
10.
Bohez L Ducatelle R Pasmans F Botteldoorn N Haesebrouck F Van Immerseel F 《Veterinary microbiology》2006,116(1-3):202-210
Invasion of Salmonella into intestinal epithelial cells is believed to be essential for the pathogenesis of Salmonella infections. Invasion is mediated by genes located on the Salmonella pathogenicity Island I (SPI-1), which are needed for assembling a type three secretion system, that mediates injection of bacterial proteins into the cytosol of epithelial cells, resulting in cytoskeletal rearrangements and as a consequence invasion. HilA is the key regulator of the Salmonella Pathogenicity Island I. To assess the role of hilA in colonization of gut and internal organs in poultry, animals were infected with 10(8) CFU of a delta hilA mutant of S. Enteritidis and its parent strain at day of hatch. Very low numbers of delta hilA mutant strain were able to colonize the internal organs shortly after infection, but they were not eliminated from internal organs at 4 weeks post-infection. At that time, the colonization level of the wild type bacteria in internal organs was decreased to the same low level compared with delta hilA mutant strain bacteria. Shedding of the delta hilA mutant strain and colonization of the caeca was seriously decreased relative to the parent strain starting from Day 5 post-infection. At 4 weeks post-infection, the delta hilA mutant strain was more or less eliminated from the chicken gut, while the parent strain was still shed to a high level and colonized the caeca to a high extent (more than 10(7) CFU/g). It is concluded that hilA is involved in long-term shedding and colonization of S. Enteritidis in the chicken caeca. 相似文献