全文获取类型
收费全文 | 16115篇 |
免费 | 908篇 |
国内免费 | 1549篇 |
专业分类
林业 | 823篇 |
农学 | 1388篇 |
基础科学 | 307篇 |
2331篇 | |
综合类 | 6049篇 |
农作物 | 1003篇 |
水产渔业 | 667篇 |
畜牧兽医 | 4005篇 |
园艺 | 1308篇 |
植物保护 | 691篇 |
出版年
2024年 | 32篇 |
2023年 | 233篇 |
2022年 | 375篇 |
2021年 | 601篇 |
2020年 | 577篇 |
2019年 | 650篇 |
2018年 | 459篇 |
2017年 | 690篇 |
2016年 | 855篇 |
2015年 | 786篇 |
2014年 | 867篇 |
2013年 | 1000篇 |
2012年 | 1223篇 |
2011年 | 1319篇 |
2010年 | 1142篇 |
2009年 | 1174篇 |
2008年 | 983篇 |
2007年 | 1085篇 |
2006年 | 914篇 |
2005年 | 641篇 |
2004年 | 517篇 |
2003年 | 406篇 |
2002年 | 306篇 |
2001年 | 248篇 |
2000年 | 241篇 |
1999年 | 212篇 |
1998年 | 118篇 |
1997年 | 123篇 |
1996年 | 125篇 |
1995年 | 109篇 |
1994年 | 74篇 |
1993年 | 82篇 |
1992年 | 70篇 |
1991年 | 61篇 |
1990年 | 52篇 |
1989年 | 43篇 |
1988年 | 41篇 |
1987年 | 29篇 |
1986年 | 27篇 |
1985年 | 20篇 |
1984年 | 12篇 |
1983年 | 6篇 |
1982年 | 4篇 |
1981年 | 5篇 |
1980年 | 5篇 |
1978年 | 5篇 |
1977年 | 4篇 |
1962年 | 4篇 |
1956年 | 9篇 |
1955年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
Zhang Wen-yu Xu Jia-hui Zhang Chun-yu Tong Hui-li Li Shu-feng Yan Yun-qin 《东北农业大学学报(英文版)》2021,28(3):38-47
Myoblast differentiation is an essential process during skeletal muscle development. C2 C12 myoblast is a commonly used experimental model to study muscle cell differentiation in vitro. Dehydrogenase/reductase(SDR family) member 3(DHRS3) is a highly conserved member in short-chain alcohol dehydrogenase/reductase superfamily and has been shown to be involved in the metabolism of retinol. Previous experimental results showed that the expression of DHRS3 increased significantly during the differentiation of myoblasts differentiation. However, the effect of DHRS3 on mouse muscle cell differentiation was unclear. The objective of current study was to determine if DHRS3 affected muscle cell differentiation, and if DHRS3 was involved in muscle regeneration. Protein expression was determined by western blot and immunofluorescence analysis. The activation and inhibition of DHRS3 increased and decreased C2 C12 myoblast differentiation respectively, which indicated that DHRS3 could affect C2 C12 myoblast differentiation. DHRS3 expression was significantly changed during muscle regeneration, with the regeneration of muscle injury, the expression of DHRS3 tended to increase first and then decrease. It suggested that DHRS3 might be involved in muscle regeneration. In summary, this study confirmed the involvement of DHRS3 in C2 C12 myoblast differentiation and mouse skeletal muscle regeneration and provided a theoretical basis for further elucidating the molecular mechanism of muscle development. 相似文献
2.
基于GF-1和Sentinel-2时序数据的茶园识别 总被引:1,自引:1,他引:0
由于茶园大多分布在地形复杂的山区,地块破碎,分布零散,形状差异大、植被混杂且茶园所处环境长期受到云雨的影响,增加了茶园遥感识别的难度与不确定性,针对这一问题,该研究提出了利用高分1号(GF-1)和哨兵2号(Sentinel-2)时序数据提取茶园的方法,以浙江省武义县王宅镇为研究区,采用GF-1号为主要数据源,并利用MODIS地表反射率产品和Sentinel-2反射率数据,基于时空融合算法得到时间分辨率5 d的10 m Sentinel-2完整的时序数据。综合利用GF-1在空间细节方面的优势和重建的Sentinel-2高观测频率时序数据在反映茶树生长过程方面的优势,分别基于GF-1的光谱和纹理特征及GF-1的光谱、纹理特征和Sentinel-2时序特征两种特征组合方式,采用随机森林算法提取茶园。结果表明,GF-1光谱、纹理信息结合Sentinel-2时序信息分类结果的准确率、错误率、精确率、召回率和F1分数分别为96.91%、3.09%、89.00%、83.09%和0.86,仅基于GF-1光谱和纹理信息的分类准确率、错误率、精确率、召回率和F1分数分别为94.72%、5.28%、73.09%、84.61%和0.78,添加时序信息分类结果总体优于未添加时序信息的分类结果。表明高空间分辨率结合高频率时序遥感数据是提高茶园分类精度的有效手段。 相似文献
3.
4.
过敏是人和养殖动物常见的疾病,而且发生的频率呈现逐渐增加的趋势。为了深入了解过敏反应的机制,本研究利用CRISPR/Cas9基因编辑技术,构建钙调磷酸酶A beta (calcineurin A beta,CnAβ)基因敲除的大鼠RBL-2H3细胞株,并探讨CnAβ基因对RBL-2H3细胞生长及脱颗粒的影响。选取大鼠CnAβ基因第一外显子为敲除靶点,设计并合成3个单导向RNA (single guide RNA,sgRNA),构建pX459-CnAβ-sgRNA质粒,并用脂质体3000将构建好的质粒转染到RBL-2H3细胞内;利用嘌呤霉素对转染细胞进行筛选,通过DNA测序验证获得CnAβ基因敲除的RBL-2H3细胞株;并检测CnAβ基因缺失对细胞增殖和脱颗粒的影响。结果表明,成功构建了CnAβ单基因敲除的大鼠RBL-2H3细胞株;CnAβ基因缺失对RBL-2H3细胞增殖、细胞的颗粒形成以及颗粒含量无显著影响,但显著抑制由细胞表面受体介导的RBL-2H3细胞脱颗粒作用。该研究结果有助于深入了解动物过敏性疾病的发生机制,为动物过敏性疾病的预防提供了理论基础。 相似文献
5.
由于饲料中多种霉菌毒素并存的几率比较高,本研究以仔猪肠上皮细胞(IPEC-J2)为模型,研究黄曲霉毒素B1(AFB1)、玉米赤霉烯酮(ZEA)和呕吐毒素(DON)的叠加细胞毒性。细胞毒性试验选用AFB1、ZEA和DON三种毒素作为响应面Box-Behnke设计的三个因素,以AFB1:10、20、30 μg/L,ZEA:150、300、450 μg/L,DON:500、1000、1500 μg/L作为Box-Behnke设计的三个编码水平。利用响应面设计构建得到17组复合霉菌毒素组合,以其对IPEC-J2细胞活力的影响作为参考指标,得到对细胞损伤程度最高和最低的霉菌毒素添加比例。结果表明:经方程预测后,得到细胞活力最低(霉菌毒素毒性最高)的AFB1、ZEA和DON组合为30、150 μg/L和1500 μg/L,经测定细胞活力为32.32%|得到细胞活力最高(霉菌毒素毒性最低)的AFB1、ZEA和DON组合为10、150 μg/L和600 μg/L,经测定细胞活力为53.01%。该结果为多种霉菌毒素叠加毒性的研究提供了依据。
[关键词] IPEC-J2细胞|黄曲霉毒素B1|玉米赤霉烯酮|呕吐毒素|细胞毒性 相似文献
6.
Yong-fang ZHANG Chun-yan ZHANG Bo ZHANG Man YIN Hui-long HONG Li-li YU Hua-wei GAO Yong-zhe GU Zhang-xiong LIU Fu-heng LI Li-juan QIU 《农业科学学报》2021,20(5):1193-1203
In order to screen the aroma characteristics of soybean,a new method was established which can quickly quantify the content of 2-acetyl-1pyrroline (2-AP),an important compound related to soybean aroma,using gas chromatography-mass spectrometry (GC-MS).Based on peak profile,total peak area and retention time as test indexes,an accurate identification method for fragrant soybeans was established.The optimum parameters of the protocol consisted of column temperature70°C,sample injector temperature 180°C,optimum extraction alcohol content 1 m L,Na Cl content 0.1 g,ultrasonication time10 min,and extraction time 1 h,which were established by using the orthogonal test of single factors and three factors with four levels (L_9(3)~4).2-AP content of leaves had significant correlations with seeds,which were easier to measure.The protocol was simple and easy to carry out,consumed only small amounts of reagents,and provided accurate and reliable results with good reproducibility.A total of 101 soybean genotypes from different geographical sources were analyzed using this protocol.The results showed that the average content of 2-AP was 0.29 mg L~(–1),ranging from 0.094 to 1.816 mg L~(–1),and the genetic diversity index was 0.54.Among all genotypes-tested,they were classified into three grades,including seven elite genotypes identified as"grade one fragrant soybeans",which were Zhonglong 608,Heinong 88,Ha13-2958,Hongmiandou,Heinong 82,Huangmaodou,and Jiyu 21.These results provide both an identification technique and several elite aroma genotypes for gene discovery and good quality breeding in soybean. 相似文献
7.
AbstractThe life history and fitness of Nilaparvata lugens being reared under ambient level (current CO2 concentration), medially elevated (550?µL/L) and highly elevated (750?µL/L) CO2 concentration for long-term generation were compared using two-sex life table instead of traditional age-specific life table. The results showed that significantly longer larval duration and lower fecundity of N. lugens were observed in 750?µL/L relative to 550?µL/L treatment (P?<?0.05). Accordingly, 550?µL/L CO2 significantly enhanced population parameters of N. lugens, including significantly higher intrinsic rate of increase (r), finite rate (λ) and net reproductive rate (RO), but not for 750?µL/L CO2. Taken together, N. lugens performs enhanced development rate, fecundity and survival in individual life history and higher potential in population multiplication under 550?µL/L CO2 level, while only indicates the less enhanced development rate and survival without significant increased capacity of population expansion under 750?µL/L CO2 level. These results should facilitate predicting the fitness and potential population damage of N. lugens, which is valuable for the integrated control of N. lugens in the future increasing CO2 concentration. 相似文献
8.
9.
自CRISPR/Cas9(clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9)基因
组编辑技术发现以来,迅速在作物中得到广泛应用。但是,CRISPR/Cas9多基因编辑系统在大豆中的研究尚待开
发。本文利用CRISPR/Cas9介导的多基因编辑系统,分别构建了两个载体,一个载体含6个靶点,编辑7个大豆基因
(4个Glycine max ASYMMETRIC LEAVES1(GmAS1)同源基因和3个GmAS2 同源基因),另一个载体含8个靶点,编辑
11个G. max AGAMOUS 家族同源基因(4个GmAG 同源基因,2个G. max SEEDSTICK(GmSTK)同源基因和5个G. max
SHATTERPROOF1(GmSHP1/2)同源基因)。大豆遗传转化后,经表型鉴定和靶点检测发现,CRISPR/Cas9介导的多
基因编辑系统在大豆中成功实现了多基因编辑。当3个GmAS1 同源基因和3个GmAS2 同源基因同时突变时,导致
大豆叶片向远轴面弯曲、皱缩且叶柄变短的表型。当2个GmSHP1 同源基因和2个GmSTK 同源基因同时突变时,导
致豆荚停止发育的不育表型。 相似文献
10.