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1.
The energy content of the mycoparasite Sporidesmium sclerotivorum mycelium was 18,389 J g?1 and 16,334 J g?1 for macroconidia on a dry weight basis. The energy content of Sclerotinia minor sclerotia, the host of the mycoparasite, was 16,485 J g?1. In liquid culture, the economic coefficient for the conversion of glucose to mycelium (mycelial dry wt ÷ glucose consumed × 100) was 51–60 whereas the mycelial energy coefficient, [mycelial energy (J) ÷ substrate energy (J) × 100] was 65–75. In soil, the conidial energy coefficient [conidial energy (J) ÷ substrate energy (J) × 100] for the conversion of host sclerotial energy to the macroconidia of the mycoparasite was 19.8, which was 2–9 times that for the conversion of glucose in liquid culture. The conidial energy coefficient when grown on a liquid medium on vermiculite was 23.0. S. sclerotivorum, as an obligate parasite of sclerotia in soil, was most efficient in the conversion of energy in a system where there was a high surface: energy ratio. In liquid culture S. sclerotivorum is more efficient than most other fungi.  相似文献   
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Seventeen isolates of the free-living soil fungus Trichoderma spp., collected from Meloidogyne spp. infested vegetable fields and infected roots in Benin, were screened for their rhizosphere competence and antagonistic potential against root-knot nematodes, Meloidogyne incognita, in greenhouse pot experiments on tomato. The five isolates expressing greatest reproductive ability and nematode suppression in pots were further assessed in a typical double-cropping system of tomato and carrot in the field in Benin. All seventeen isolates were re-isolated from both soil and roots at eight weeks after application, with no apparent crop growth penalty. In pots, a number of isolates provided significant nematode control compared with untreated controls. Field assessment demonstrated significant inhibition of nematode reproduction, suppression of root galling and an increase of tomato yield compared with the non-fungal control treatments. Trichoderma asperellum T-16 suppressed second stage juvenile (J2) densities in roots by up to 80%; Trichoderma brevicompactum T-3 suppressed egg production by as much as 86%. Tomato yields were improved by over 30% following the application of these biocontrol agents, especially T. asperellum T-16. Although no significant effects were observed on carrot galling and yield, soil J2 densities were suppressed in treated plots, by as much as 94% (T. asperellum T-12), compared with the non-fungal controls. This study provides the first information on the potential of West-African Trichoderma spp. isolates for use against root-knot nematodes in vegetable production systems. The results are highly encouraging, demonstrating their strong potential as an alternative and complementary crop protection component.  相似文献   
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Studies of biological responses in the terrestrial environment to rapid changes in climate have mostly been concerned with above-ground biota, whereas less is known of belowground organisms. The present study focuses on enchytraeids (Oligochaeta) of heathland ecosystems and how the enchytraeid community has responded to simulated climate change in a long-term field experiment. Either increased temperature or repeated drought was applied for 13 years to field plots located in Wales, The Netherlands and Denmark representing a gradient in precipitation and annual temperature fluctuations thereby providing an opportunity to study biological responses on a local (within sites) and regional scale. Warming treatments increasing night-time temperature (0.5–1 °C higher than ambient at 5 cm soil depth) had no detectable effects on the enchytraeid communities. Increased intensity and frequency of drought had rather weak persistent effects on total enchytraeid abundance suggesting that ecosystem functions of enchytraeids may only be transiently impacted by repeated spring or summer drought. However, drought treatment had persistent negative effects on species richness and community structure across sites. Drought treated plots harboured only 35–65% of the species present in control plots, and the reduction of species richness was most pronounced at the driest sites. It is discussed that soil invertebrates, due to their weak migratory potential, may be more liable to extinction under changing climatic conditions than above-ground species, and therefore consequences of climate change to soil organisms need particular attention in future research.  相似文献   
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Eucalyptus spp. are among the most widely planted exotic trees in Ethiopia. Several damaging leaf pathogens are known from Eucalyptus spp. worldwide. Of these, Mycosphaerella spp. are among the most important, causing the disease known as Mycosphaerella leaf disease (MLD). Characteristic symptoms of MLD include leaf spot, premature defoliation, shoot and twig dieback. Recent disease surveys conducted in Ethiopian Eucalyptus plantations have revealed disease symptoms similar to those caused by Mycosphaerella spp. These symptoms were restricted to E. globulus trees growing in several localities in south, south western and western Ethiopia. The aim of this study was to identify the fungi associated with this disease. This was achieved by examining ascospore germination patterns, anamorph associations and sequence data from the Internal Transcribed Spacer (ITS) region of the rRNA operon, for representative isolates. Several different ascospore germination patterns were observed, suggesting that more than one species of Mycosphaerella is responsible for MLD on E. globulus in Ethiopia. Analysis of sequence data showed that three Mycosphaerella spp., M. marksii, M. nubilosa and M. parva were present. This is the first report of these three species from Ethiopia and represents a valuable basis on which to build further studies in the region.  相似文献   
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Tian  Zhenqi  Chen  Guangmei  Zhang  Yan  Ma  Chao  Tian  Zhenya  Gao  Xuyuan  Chen  Hongsong  Guo  Jianying  Zhou  Zhongshi 《Journal of pest science》2022,95(3):1233-1244

Low winter temperatures severely stress newly arriving insect species. Adaptive evolutionary changes in cold tolerance can facilitate their establishment in new environments. Ambrosia artemisiifolia, a noxious invasive plant, occurs throughout China. Ophraella communa, a biological control agent of A. artemisiifolia, mainly occurs in southern China. However, in 2012, it established populations in Beijing (39.98°N, 115.97°E) following introduction from Laibin (23.62°N, 109.37°E), implying cold adaptation. The mechanisms underlying its rapid evolution of cold tolerance remain unknown. We investigated the levels of cryoprotectants and energy reserves in adult O. communa from two latitudes. In high-latitude insects, we found high trehalose, proline, glycerol, total sugar, and lipid levels; five potential genes (Tret1a, Tret1b, Tret1-2, P5CS, and GST), responsible for regulating cold tolerance and involved in trehalose transport, proline biosynthesis, and glutathione S-transferase activation, were highly expressed. These hybridisation changes could facilitate cold temperature adaptation. We demonstrate the genetic basis underlying rapid adaptation of cold tolerance in O. communa, explaining its extension to higher latitudes. Thus, specialist herbivores can follow host plants by adapting to new temperature environments via rapid genetic evolution.

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Chromolaena odorata (L.) K. & R. (= Eupatorium odoratum L.) has invaded large areas of the coastal belt of Natal province in South Africa. Propagation is predominantly by means of windborne achenes. The germination of these achenes was investigated. Of the temperatures tested, alternating 15/30°C was optimal for germination. These achenes are positively photoblastic and an imbibition period prior to exposure was required to sensitize the achenes to light. The promotive effect of red light on the germination of imbibed achenes could be reversed by subsequent exposure to far-red light while the inhibitory effect of far-red light could be nullified by a subsequent red light treatment. This is typical of a phytochromemediated germination response. Germination d'akènes de Chromolaena odorata (L.) K. & R.: effets de la température, de l'imbibition el de la lumière Chromolaena odorata (L.) K. & R. (= Eupatorium odoratum L.) a envahi de grandes superficies dans le littoral de la province de Natal en Afrique du Sud. La plante se répand surtout par moyen d'akènes portés par le vent. La germination de ces akènes a fait l'objet d'une étude. Parmi les températures essayées, 15/30°C s'est révélée optimale pour la germination. Les akènes font preuve d'une photoblasticité positive et pour les sensibiliser à la lumière, il leur faut une période d'imbibition avant de les y exposer. La promotion de germination provoquée par la lumière rouge chez les akènes imbibés a pu etre inversée par une exposition ultérieure à la lumière extrême-rouge (700 à 750 nm) tandis que l'effet inhibiteur de celle-ci s'est trouvé annulé par un traitement ultérieur à la lumière rouge. Cette réaction est caractéristique d'une germination réglée par le phytochrome. Die Keimung von Achaenen von Chromolaena odorata (L.) K. & R.: der Einfluss von Temperatur, Quellung und Licht. Chromolaena odorata (L.) K. & R. (= Eupatorium odoratumL.) hat sich auf grossen Flächen des Küstengürtels der Provinz Natal (Südafrika) ausgebreitet. Durch den Wind verbreitete Achaenen sind hauptsächlich für die Vermehrung verantwortlich. Thema der vorliegenden Untersuchung war die Keimung dieser Achaenen. Alternierende Temperaturen von 15° und 30°C waren optimal für die Keimung. Die Achaenen waren positiv photoblastisch; eine Quellungsperiode war notwendig um die Achaenen für die Lichtreaktion zu sensibilisieren. Der die Keimung vorgequollener Achaenen fördernde Einfluss von Rotlicht konnte durch eine nachfolgende‘Fern-Rot'-Exposition rückgängig gemacht werden. Hingegen wurde der Hemmungseffekt von ‘Fern-Rot’ durch eine darauffolgende Rotlicht-Exposition aufgehoben. Dieser Befund ist charakteristisch für eine phytochromregulierte Samenkeimung.  相似文献   
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Uromycladium acaciae is the cause of a severe wattle rust epidemic in plantations of Acacia mearnsii (black wattle) in southern Africa. Research on the biology of this damaging rust is assisting in the development of control strategies. One strategy under investigation is the identification and deployment of resistant lines of A. mearnsii. Selection of resistant families currently relies on large-scale, time-consuming and expensive field trials. In this study, we present a detailed artificial inoculation protocol for U. acaciae, which can be used to screen for resistance. The results of an experiment that used the protocol to screen the relative resistance of 12 families of A. mearnsii to U. acaciae are also presented. The developed artificial inoculation protocol can also be used to investigate several other aspects of this host–pathogen system.  相似文献   
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Euonymus bungeanus plants exhibiting symptoms of abnormal branches, small leaves and phyllody, which is indicative of E. bungeanus witches’ broom (EbWB) disease, have recently been found in Beijing, China. A phytoplasma from symptomatic E. bungeanus plants was identified by 16S rRNA polymerase chain reaction (PCR) using the phytoplasma‐specific universal primer pair R16mF2/R16mR1. Inoculation of healthy E. bungeanus plants by grafting with diseased scions was also performed. The rp and secY genes of the EbWB phytoplasma were cloned and sequenced as was the 16S rRNA gene. Sequence and phylogenetic analyses of 16S rRNA, rp and secY genes indicated that the phytoplasma associated with E. bungeanus belongs to the 16SrV‐B, rpV‐C and secY‐C subgroup, the same subgroup as the jujube witches’ broom (JWB) phytoplasma that is widely distributed among jujube trees in China. Comparative analyses based on virtual restriction fragment length polymorphism (RFLP) showed that the EbWB phytoplasma is more closely related to another 16SrV‐B subgroup strain: RPWB (Robinia pseudoacacia witches’ broom). To the best of our knowledge, this is the first report of a witches’ broom phytoplasma in E. bungeanus in China, and the findings add a new cultivated plant species to the already broad natural host range of 16SrV‐B subgroup phytoplasmas.  相似文献   
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