不同辣椒种质对象耳豆根结线虫的抗性评价

近年来象耳豆根结线虫的危害日益严重.为筛选抗象耳豆根结线虫的辣椒种质,本试验利用种属特异性引物对根结线虫病原物开展分子鉴定,确定为象耳豆根结线虫.接着采用室内人工接种法,对10份一年生辣椒种质和10份中国辣椒种质进行象耳豆根结线虫抗性鉴定,计算根结指数和卵粒指数,并通过比较隶属函数,发现其中3份辣椒种质对象耳豆根结线虫表现为高抗,抗病性最强的是L518M和L525-1M,L42M次之;10份表现为中抗;7份表现为感病,抗病能力最弱的是L69-1M;未发现免疫品种.本研究发现中国辣椒种质的抗病性整体高于一年生辣椒,是重要的抗病种质来源,可用于象耳豆根结线虫抗病育种和后续抗病机理的研究.     

滇中冷凉山区不同播期反季花椰菜产量效益分析

为摸索滇中高海拔冷凉山区反季节栽培花椰菜的最佳播期以集成高效栽培技术推广应用,于2017—2018年选择海拔2250 m的云南省峨山县塔甸镇大西村地块进行9个播期的2年随机区组试验。结果表明,花椰菜生育期随播期推迟而延长,而花球采收期除播期7月10日外随播期推迟而逐渐增长;花椰菜株高、外叶数、开展度、球高、球径和单球重等农艺性状有随播期延迟呈现先逐渐减小而后又逐渐增大的趋势;莲座期黑腐病和霜霉病的病情指数随着播期的延迟呈现先逐渐升高而后又逐渐下降的趋势;花椰菜小区产量随着播期的延迟呈现先逐渐下降而后又逐渐提高的趋势,播期4月20日和4月30日与其余7个播期产量之间的差异达极显著水平。综合花椰菜在冷凉山区反季节栽培的生产实际和各播期产量产值及商品性表现,推荐滇中高海拔冷凉山区反季节栽培花椰菜的最佳播期为4月20—30日。     

丛生竹林下竹荪富硒菌棒覆土栽培技术

在丛生竹林下对不同基质配方和硒浓度菌棒进行埋棒栽培以及覆土方式栽培试验，结果表明，菌棒不同基质配方对竹荪产量及其营养成分有显著影响，而且三列浅沟形和双列龟背形2种覆土方式有利于提高竹荪产量。改良基质配方菌棒竹荪产量比常规配方提高约50%；在添加外源硒质量分数为0~2.0 mg/kg的浓度范围内，竹荪子实体产量先随着硒浓度的增加而逐渐增加后再有所降低，基质中添加1.5 mg/kg硒肥比不添加产量提高了195.30%。基质中添加硒质量分数为1.0~2.0 mg/kg的硒肥可以较显著地提高竹荪花的硒含量，其干物质中硒含量平均值从约2.50 mg/kg提高到8.05~13.30 mg/kg，外源硒肥利用率达到9.70%~15.36%。菌棒不同基质配方对竹荪子实体的粗蛋白及粗多糖含量有明显影响，改良配方竹荪蛋与竹荪花的粗蛋白含量是常规配方的1.21和1.29倍，其粗多糖含量是常规配方的4.81和1.35倍；基质中添加硒肥与不添加硒肥相比，竹荪蛋与竹荪花中粗蛋白含量分别增加了40.90%和14.30%。在硒质量分数为1.0 mg/kg的竹荪菌棒林下覆土栽培试验中，三列浅沟形覆土方式单位面积鲜竹荪蛋产量最高，为10.27 kg/m²；双列龟背形覆土方式单个菌棒的鲜竹荪蛋产量最高，达1.40 kg。三列浅沟形和双列龟背形2种覆土方式基质生物转化率分别为93.00%和94.14%，达到了较高水平。     

极小种群植物“川柿”生物学特性及种子萌发方法研究

摘 要：【目的】为了保护和发展极小种群植物川柿，开展此研究。【方法】采用观测法，连续3年对其生物学特性研究；种子萌发方法：（1）后熟0、30、45、60天，剥离清洗播种；（2）后熟60天，以GA300、600、1000 mg/L溶液浸种1h；播种基质为河沙（保持一定湿度），放置室内，统计萌发时间和出苗率。【结果】（1）川柿花期4-5月，10月为果熟期，单果直径32.7±5.80mm，单果重23.0±5.30g；种子肾形、千粒重1070g；成年植株呈隔年开花、结果现象；（2）种子经“后熟+河沙播种”，可萌发，其中以后熟60天的出苗率较高，为65%；直接播种种子，出苗率5%；“后熟+GA溶液浸种+河沙播种”，出苗率均高于65%，其中以GA600mg/L浸泡1h处理，出苗率最高为83.3%。【结论】极小种群植物川柿为常绿叶乔木，有隔年开花、结果现象； “果后熟60天+GA600mg/L浸泡种子1h +室内河沙播种”可提高种子萌发率，为川柿种子繁殖提供参考。     

不同行距配比对玉米光合生理指标及茎秆特性的影响

以‘大丰30’为试验材料,在同一密度下（75000株/hm²）设置5种不同行距配比,研究不同行距配比对玉米光合生理指标及茎秆力学特性的影响。结果表明：行距配比为40 cm×80 cm时,棒三叶的叶绿素含量(SPAD)在灌浆期达到峰值;行距配比为30 cm×90 cm时,棒三叶净光合速率在灌浆期最高。大口期开始,行距配比为40 cm×80 cm时,叶面积指数(LAI)最高。单株干物质积累量以40 cm×80 cm最高。各行距配比的茎秆硬皮穿刺强度与茎秆弯曲性能随着节位的下降均呈逐渐增强的趋势,从大到小为 40 cm×80 cm>50 cm×70 cm>30 cm×90 cm>60 cm×60 cm>20 cm×100 cm。穗长、穗粗、千粒重和穗粒数各处理间均有不同变化,行距配比为40 cm×80 cm时产量最高,比等行距60 cm×60 cm增产7.96%。由此可见,行距配比为40 cm×80 cm,有利于大穗型品种‘大丰30’的光合性能和茎秆强度的提高,从而达到抗倒伏增产的目的。     

Comparative study of the stoichiometric characteristics of karst and non-karst forests in Guizhou,China

Carbon(C), nitrogen(N), and phosphorous(P) levels and their stoichiometry in plant components(leaves, branch trunks, roots) of trees in a karst forest and non-karst forest are compared. The results show that the C contents, C:N and C:P ratios of dominant species in the karst forest were lower than those in the non-karst forest,but the N and P and the N:P ratio were higher; C:N:P ratios in plant organs of trees in the karst forest were in the order of trunksrootsbranchesleaves. However, C:N:P ratio in the non-karst forest trees were trunksbranchesrootsleaves. Moreover, ratio of C:N:P in trunks was highest and lowest in leaves in both forests. In non-karst forest trees, N:P was in the order of leavesroots branches trunks. There were no significant differences in the ratio of N:P in different plant components of trees in the karst forest. However, in karst and non-karst forest trees, the ratio of N:P in leaves was highest; positive correlations between N and P contents, and N and N:P ratios were observed in both karst and non-karst forests(p0.001). Negative correlations between P and N:P ratios(p0.05) were observed in karst forest trees, while positive correlations were observed in non-karst forest trees.     

Tree-ring response of Larix chinensis on regional climate and sea-surface temperature variations in alpine timberline in the Qinling Mountains

Tree-ring width chronologies of Larix chinensis were developed from the northern and southern slopes of the Qinling Mountains in Shaanxi Province,and climatic factors affecting the tree-ring widths of L.chinensis were examined.Correlation analysis showed that similar correlations between tree-ring width chronologies and climatic factors demonstrated that radial growth responded to climate change on both slopes.The radial growth of L.chinensis was mainly limited by temperature,especially the growing season.In contrast,both chronologies were negatively correlated with precipitation in May of the previous year and April of the current year.Spatial climate-correlation analyses with gridded land-surface climate data revealed that our tree-ring width chronologies contained a strong regional temperature signal over much of northcentral and eastern China.Spatial correlation with seasurface temperature fields highlights the influence of the Pacific Ocean,Indian Ocean,and North Atlantic Ocean.Wavelet coherence analysis indicated the existence of some decadal and interannual cycles in the two tree-ring width chronologies.This may suggest the influences of El Nin˜o-Southern Oscillation and solar activity on tree growth in the Qinling Mountains.These findings will help us understand the growth response of L.chinensis to climate change in the Qinling region,and they provide critical information for future climate reconstructions based on this species in semi-humid regions.     

复垦年限及植被模式对煤矿复垦土壤微生物多样性的影响

了解露天煤矿区复垦状况及其对土壤微生物的影响机制,对于估算受损生态系统的恢复程度至关重要。该文以采用多种模式复垦的安太堡露天煤矿为研究区域,通过野外调查与采样分析,借助于Biolog EcoPlatesTM方法对复垦区和原地貌(对照)表层土壤中的微生物功能多样性状况进行了探索,评价了复垦年限和复垦植被模式对土壤微生物特征的恢复效应;并从土壤物理化学性质(土壤容重、pH值、土壤有机碳、总氮、速效氮、速效磷和速效钾)和生物性质(土壤微生物量碳和微生物量氮)中筛选可反映土壤微生物功能多样性变化的代表性指示物质。结果表明:1)露天煤矿复垦年限和复垦植被模式均对土壤微生物的功能多样性有明显影响。复垦年限越长的样地中土壤微生物有较高的活性和均匀度,但丰富度较低;若样地的复垦年限较长,复垦植物种植模式对土壤微生物多样性的作用强于复垦时长对其的影响。2)从土壤微生物多样性恢复层面分析,单一植物复垦配置的优化次序为杏树、榆树和柠条,混交林配置较优的选择为柠条×刺槐。3)土壤微生物生物量碳的含量与土壤微生物功能多样性指标Shannon-Wiener指数(ρ=–0.69,P0.01)和McIntosh指数(ρ=–0.69,P0.05)显著负相关,在一定程度上可作为复垦区生物多样性指示物质。研究成果将为露天煤矿及其所在区域的生态恢复、治理和监测提供参考。     

空中森林步道发展概况与功能特点分析

空中森林步道是穿行于林冠层的一种独特的林中线路形式，是近年来新兴的一种步道形式，受到公众的喜爱。文中在概述国内外空中森林步道建设与发展概况的基础上，阐述了空中森林步道的4个特点:1）穿行林冠层，生态康养价值高；2）路面平缓，适宜人群广；3）视线开阔，景观丰富度高；4）受有毒、有害因素影响小，安全性高。分析了空中森林步道在视觉景观、运动游憩、康养、科普教育4个方面的主要承载功能，并对今后我国空中森林步道的建设提出展望，以期为我国空中森林步道的设计和建设提供参考。