土壤铅和镉溶出伏安法检测中影响因素及其削弱方法研究进展

溶出伏安法具有分析速度快、成本低、灵敏度高等优点被广泛应用于检测土壤重金属含量，但在检测土壤重金属时，溶出伏安检测精度会受到多方面因素的影响。该文在介绍溶出伏安法工作原理的基础上，从伏安参数、试验条件和土壤物质成分三方面阐述溶出伏安法检测土壤Pb2+和Cd2+为的影响因素，解析各因素的影响机理，归纳影响削弱方法的研究进展。研究结论为：方波脉冲阳极溶出伏安法最常用于检测土壤Pb2+和Cd2+，伏安参数包括脉冲幅值、电压增量和脉冲频率，试验条件包括沉积时间、沉积电压和支持电解质种类及其pH值，土壤成分主要干扰因素包括非目标重金属和有机质。针对伏安参数和试验条件的影响可以设计优化试验有效削弱。针对非目标重金属和有机质的干扰影响，目前研究还没有提出有效的削弱方法。最后，展望了溶出伏安法检测土壤重金属的未来发展方向。     

广西某食蟹猴规模化养殖场饮用水和青饲料卫生质量监测

为了对广西某规模化食蟹猴养殖场饮用水和青饲料卫生质量情况进行监测,对饮用水和青饲料进行连续抽样监测,按照《生活饮用水卫生标准》《生活饮用水卫生标准检验方法》和《国家禁用和限用的农药名单》进行检测与评价。结果显示:抽取6份饮用水样检测26项理化指标,有3份不合格,超标率达50.0%,超标指标有浑浊度、肉眼可见物;抽取147份饮用水检测微生物指标,有22份超标,超标率达15.0%,超标微生物指标为细菌总数、总大肠菌群、耐热大肠菌群和大肠埃希氏菌;抽取13个品种青饲料48份检测10种禁用农药残留情况,甲胺磷、敌敌畏、乐果、氧化乐果、氰戊菊脂、多菌灵、啶虫脒、吡虫啉、三唑磷、毒死蜱检出率分别为2.1%、0、0、0、0、12.5%、2.1%、12.5%、2.1%、2.1%。说明该规模化食蟹猴养殖场饮用水存在不合格情况,青饲料检出农药残留。     

The first survey and molecular identification of Entamoeba spp. in farm animals on Qinghai-Tibetan Plateau of China

Protozoans of Entamoeba spp. are globally distributed zoonotic parasites that infect diverse animal hosts and humans. Prevalence and species/genotypes distribution of Entamoeba spp. in domestic animals are not fully investigated on Qinghai-Tibetan Plateau (QTP), an animal husbandry and agriculture region of China. In a survey, 528 fecal samples were collected from 7 species of domestic animals on multiple locations across QTP region and analyzed by PCR and sequencing analysis. The overall prevalence of Entamoeba spp. infection in all examined animals was 97.9 %. Four Entamoeba species, E. bovis, E. moshkovskii, E. ecuadoriensis and E. histolytica were found, and majority (94.2 %) of Entamoeba-infected animals harbored E. bovis. Six Entamoeba genotypes, Entamoeba ribosomal lineages (RL) 1, 2, 3, 4, 8 and 9 were identified by sequencing analysis. Two zoonotic species, E. moshkovskii and E. histolytica, were present in horses, while E. ecuadoriensis and E. bovis were found in horses and all species of seven farm animals, respectively. It was also observed that six Entamoeba genotypes were distributed in animals in specific pattern. The results revealed high prevalence of Entamoeba spp. infection in livestock, broad range of hosts as well as diversity and species/genotype distribution of Entamoeba spp. in farm animals inhabiting on the high altitude QTP region.     

不同温度下褐带卷蛾茧蜂实验种群生命表

为了探索温度对褐带卷蛾茧蜂Bracon adoxophyesi发育和繁殖的影响,研究了该蜂在6种温度下的实验种群生命表。结果表明,褐带卷蛾茧蜂各虫态发育历期随温度升高而缩短,在36℃下产卵前期略高于32℃下的产卵前期,说明温度过高或者过低均不利于卵巢发育。随温度降低,其世代历期显著延长,说明低温会显著抑制该蜂发育。各虫态的发育速率与温度的关系符合Logistic模型。该寄生蜂产卵前期的发育起点温度高于其他阶段的发育起点温度。在24℃下单雌产卵量最高,为619.86粒,16℃和36℃下的产卵量最低,分别为139.60粒和154.43粒。在24、28和32℃时,其种群趋势指数分别为101.78、74.25和73.41,在36℃和16℃时,其种群趋势指数较低,说明温度过高或者过低均不利于其种群繁殖,且低温的影响大于高温。在24~32℃下,褐带卷蛾茧蜂的种群趋势指数较高,说明24~32℃是适宜该寄生蜂种群增长的温度范围。     

Herbicide resistance costs: what are we actually measuring and why?

Despite the considerable research efforts invested over the years to measure the fitness costs of herbicide resistance, these have rarely been used to inform a predictive theory about the fate of resistance once the herbicide is discontinued. One reason for this may be the reductive focus on relative fitness of two genotypes as a single measure of differential performance. Although the extent of variation in relative fitness between resistant and susceptible plants has not been assessed consistently, we know enough about plant physiology and ecology not to reduce it to a single fixed value. Research must therefore consider carefully the relevance of the experimental environment, the life stage and the choice of metric when measuring fitness‐related traits. The reason most often given for measuring the cost of resistance, prediction of the impacts of management options on population dynamics, cannot be addressed using arbitrary components of fitness or a fixed value of relative fitness. To inform management options, the measurement of traits that capture the relevant processes and the main causes of their variation are required. With an emphasis on the benefit of field experiments measured over multiple time points and seasons, we highlight examples of studies that have made significant advances in this direction. © 2017 Society of Chemical Industry     

独立学院“生物化学”综合性实验的开设

从综合性实验内容、综合性实验开设对人才培养目标的体现、综合性实验实施过程中的注意事项等方面阐述如何在“生物化学”课程中开设综合性实验“聚丙烯酰胺凝胶电泳分离蛋白及蛋白质含量的测定”。此实验的开设,可使学生将理论知识融会贯通,对培养学生的动手能力和创新能力有积极作用。     

正交试验设计中正交表的特点及正交的意义浅析

通过正交试验设计可以大大减少试验次数,得到不同因素水平的最优组合,从而确定合理的试验方案及工艺条件等,因此在科学研究中应用非常广泛。为了更深入地理解正交试验设计方法,以表和图的形式对正交试验设计的特点进行了详细分析;对正交性的意义、体现从向量信息不相关及向量内积计算的角度进行详细的分析及解释,以期为广大师生的学习及应用提供一定的帮助。     

残塬沟壑区果园复合种植对土壤动物群落特征的影响

为了解苹果复合种植即果-蔬[MB]、果-草[MH]、果-荒[MW]、果-粮[MZ]模式下土壤动物群落结构特征,采用手拣法和改良干、湿生漏斗法,对黄土残塬沟壑区苹果园4种复合种植模式下的土壤动物群落组成及特征进行调查研究。4种模式下共分离得到土壤动物57.33百只/m~2,隶属4门11纲23目42个类群。4种复合种植模式下土壤动物个体密度和类群数的垂直分布特征表现出明显的表聚特征,水平分布特征表现为果-草[MH]果-蔬[MB]果-荒[MW]≥果-粮[MZ]。果-草[MH]复合种植模式下土壤动物的多样性指数、均匀度指数及丰富度指数最高,优势度指数最低,表明4种复合种植模式对果园土壤动物类群多样性的影响呈现不同的特征。研究结果可为黄土残塬沟壑区苹果果园生物多样性保护提供土壤动物生态学依据。     

植物根系对土壤团聚体形成作用机制研究回顾

土壤团聚体是土壤结构的基本单元,土壤水力侵蚀的微观描述即为土壤团聚体的破裂过程。研究表明:植物根系可以改变土壤的力学以及水文特征,促进土壤团聚体的形成和稳定。因此,对近20年国内外的相关研究成果进行较为系统的回顾,从根系对土壤团聚体的物理、生物、电化学作用3个研究视角,分析了植物根系对土壤团聚体形成的作用机制,提出了现有研究中存在的问题及研究趋势,这对深入认识植物根系对土壤团聚体的影响及其作用机制、发展根-土相互作用的土壤侵蚀过程模型具有重要的意义。