Development of a reverse-transcription loop-mediated isothermal amplification assay to detect avian influenza viruses in clinical specimens

In recent years, the avian influenza has brought not only serious economic loss to the poultry industry in China but also a serious threat to human health because of the avian influenza virus(AIV) gene recombination and reassortment. Until now, traditional RT-PCR, fluorescence RT-PCR and virus isolation identification have been developed and utilized to detect AIV, but these methods require high-level instruments and experimental conditions, not suitable for the rapid detection in field and farms. In order to develop a rapid, sensitive and practical method to detect and identify AIV subtypes, 4 specific primers to the conserved region of AIV M gene were designed and a loop-mediated isothermal amplification(RT-LAMP) method was established. Using this method, the M gene of H1–H16 subtypes of AIV were amplified in 30 min with a water bath and all 16 H subtypes of AIV were able to be visually identified in presence of fluorescein, without cross reaction with other susceptible avian viruses. In addition, the detection limit of the common H1, H5, H7, and H9 AIV subtypes with the RT-LAMP method was 0.1 PFU(plaque-forming unit), which was 10 times more sensitive than that using the routine RT-PCR. Further comparative tests found that the positivity rate of RT-LAMP on detecting clinical samples was 4.18%(14/335) comparing with 3.58%(12/335) from real-time RT-PCR. All these results suggested that the RT-LAMP method can specifically detect and identify AIV with high sensitivity and can be considered as a fast, convenient and practical method for the clinic test and epidemiological investigation of AIV.     

药用植物腊叶塑封标本制作技术

提出了药用植物腊叶塑封标本制作中采集——压制——装订上台纸——塑封等各环节的方法,尤其针对标本制作中存在的变色、叶片脱落等问题提出了用5%酒精溶液浸泡、0.3%沸盐水浸泡、烘干箱烘干、硅胶干燥、醋酸铜溶液保色等解决方案。     

猪卵母细胞在不同条件下的体外成熟培养

研究了不同时间段添加外源激素及不同基础培养液和血清有无对猪卵母细胞体外成熟的影响。实验包括（1）猪卵母细胞以TCM199＋10％FBS＋10IU／mL pregnant more serum gonadotrophin（PMSG）＋10IU／mL human chorion gonadotrophin（hCG）＋2．5IU／mL follicle stimulate hormone（FSH）为成熟培养液体外培养44h，前22h在培养液中加激素，后22h不加激素及前22h不加激素，后22h添加激素和添加激素连续培养44h；（2）以实验（1）中激素添加的方案在体外添加激素连续培养44h，对不同基础培养液（TCM199粉剂、TCM199原液和改良TCM199（mM199））对猪卵母细胞体外成熟进行了研究；（3）以实验（2）中筛选出的mM199组为成熟培养液对比血清有无对猪卵母细胞体外成熟的影响。结果表明，实验（1）中3种激素添加不同时间段其成熟率分别为45．67％、45．29％和46．07％，对猪卵母细胞体外成熟无显著影响（P〉0．05）；实验（2）中，mM199组的成熟率（54．10％）高于TCM199粉剂组的成熟率（46．16％）及TCM199原液组的成熟率（47．14％），但差异不显著（P〉0．05）；实验（3）中无血清组的成熟率（67．10％）高于有血清清组的成熟率（52．22％），差异显著（P〈0．05）。由此表明，mM199＋10IU／mL PMSG＋10IU／mL hCG＋2．5IU／mL FSH是一种适合于猪卵母细胞体外成熟的培养液，体外成熟率达67．10％。     

动物标本在动物学教学中的作用

动物标本是动物学教学中最好、最直观的教具和观察材料．主要种类有浸制标本、剥制标本、骨骼标本、干制标本和玻片标本等。广西大学动物学标本室保存了动物标本1350种．3276份．包括虎纹捕鸟蛛、虎纹蛙、金钱龟、蟒蛇、巨蜥、海南虎斑璃、金雕、黑熊、穿山甲、克隆牛和白头叶猴等珍稀物种。动物标本在动物学教学中的作用，主要是使动物学教学更为直观和形象．活跃动物学教学的课堂气氛．增加学生学习动物学的浓厚兴趣以及提高学生的动手能力。     

中草药饲料添加剂改善禽肉品质的研究

通过对近年来相关文献的整理分析，归纳总结了一些中草药对禽肉品质产生的影响及其实现途径，为开发味美且绿色安全的优质禽肉产品提供思路。     

Enhanced micropropagation of Dendrobium huoshanense C.Z. Tang et S.J. Cheng through protocorm-like bodies: The effects of cytokinins,carbohydrate sources and cold pretreatment

The effects of cytokinins, carbohydrate sources and cold pretreatment on the conversion of protocorm-like bodies (PLBs) to shoots were investigated for the enhancement of micropropagation of Dendrobium huoshanense C.Z. Tang et S.J. Cheng, an endangered medicinal plant in China. The effects of cytokinins and carbohydrate sources on the conversion of PLBs to shoots depended on their types and concentrations. The best results in terms of shoot development from PLBs occurred on 1/2 MS medium supplemented with 20 μM kinetin and 10 g l⁻¹ maltose. Cold pretreatment at 10 °C for 1–2 weeks significantly enhanced the conversion of PLBs to shoots, and over 1300 shoots were obtained from one gram of PLBs after 3 months of culture. The developed shoots were rooted on growth regulator-free MS medium supplemented with 8 g/l banana paste to give complete plantlets, which were successfully acclimatized with a survival rate of approximately 65%. The results indicate that a suitable cold pretreatment (10 °C for 1 week) followed by the use of 20 μM kinetin and 10 g/l maltose in 1/2 MS medium would produce a large number of shoots from PLBs for plantlet regeneration of D. huoshanense.     

中医院校图书馆参考咨询员知识结构分析

 

从医、教、研人员对中医古籍图书的需求、对中医古代专题资料的需求、对现代中医药期刊文献的需求、对特种中医药文献的需求、对中医药数据信息的需求、对中医药课题研究的定性需求等实例出发，对中医院校图书馆参考咨询员的知识结构进行了分析。

     

用人工神经网络模拟土体塌陷的可行性分析

探讨了利用人工神经网络预测土体塌陷与土体参数和荷载条件之间关系的可行性。BP神经网络是最常用的神经网络之一,通过网络训练,最终确定了6个变量即输入信息、8个隐层和1个输出层的网络结构。在比较了预测值与试验值后,进一步证实了人工神经网络在评估土体塌陷方面效果明显。     

泡沫塑料雕塑技术在鸵鸟形态标本制作中的应用研究

传统的形态标本制作是用棉花稻草等物做填充,或用钢筋、木头、石膏做成假体,存在着形态不逼真、易变形、易发霉、假体的制作工艺复杂、耗材多、重量重、易破损等缺点。本试验采用塑料泡沫雕塑技术成功制作出鸵鸟形态标本,具有形态逼真、不易变形、不易霉变、不易损坏、重量轻、易加工、工艺简单等优点。本试验对鸵鸟养殖业的生产、教育、科研及科学普及都具有积极的意义,对动物形态标本的制作也起到了积极作用。