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Selecting beneficial DNA variants is the main goal of animal breeding. However, this process is inherently inefficient because each animal only carries a fraction of all desirable variants. Genome editing technology with its ability to directly introduce beneficial sequence variants offers new opportunities to modernize animal breeding by overcoming this biological limitation and accelerating genetic gains. To realize rapid genetic gain, precise edits need to be introduced into genomically-selected embryos, which minimizes the genetic lag. However, embryo-mediated precision editing by homology-directed repair (HDR) mechanisms is currently an inefficient process that often produces mosaic embryos and greatly limits the numbers of available edited embryos. This review provides a summary of genome editing in bovine embryos and proposes an embryo-mediated accelerated breeding scheme that overcomes the present efficiency limitations of HDR editing in bovine embryos. It integrates embryo-based genomic selection with precise multi-editing and uses embryonic cloning with elite edited blastomeres or embryonic pluripotent stem cells to resolve mosaicism, enable multiplex editing and multiply rare elite genotypes. Such a breeding strategy would enable a more targeted, accelerated approach for livestock improvement that allows stacking of beneficial variants, even including novel traits from outside the breeding population, in the most recent elite genetic background, essentially within a single generation.  相似文献   
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基因组编辑技术应用于作物遗传改良的进展与挑战   总被引:5,自引:0,他引:5  
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何华兰 《现代农业科技》2014,(5):274-275,278
介绍了TAL效应蛋白相关的研究进展,包括TAL效应蛋识别DNA,其在基因工程上的应用,TALENs中DNA结合域的设计和组装,并对未来发展趋势进行分析和展望,以期为TALENs技术的发展与应用提供参考。  相似文献   
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This study aims to establish a reporting system for stem cells tracer, EGFP cDNA together with LoxP-flanked PGK-neo cassettes were inseet into the Oct-4 gene of Wuzhishan Miniature pig at the stop codon via TALENs mediated homologous recombination, expression of EGFP gene was under control of Oct-4 5'-regulatory region. TALENs directed against sequences flanking the stop codon of Oct-4 were cotrasfected with targeting vector, 514 drug-resistant cell clones were screened, and 36 correctly targeted clones were confirmed by PCR from them, the targeting efficiency were 5.6% and 13.0%. Oct-4-EGFP transgenic cell line was establinshed in this study, and it was also proved that TALENs could significantly improve the efficiency of homologous recombination.  相似文献   
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基因敲除技术最新研究进展及其应用   总被引:1,自引:0,他引:1  
陶果  信吉阁  肖晶  刘海京  成文敏  查星琴  曾养志 《安徽农业科学》2013,(29):11605-11608,11644
基因敲除技术是基因功能研究的最直接和有效的方法之一。近年来发展起来多种基因组功能性研究的高效工具,包括ZFNs、TALENs和Cas9等,为高效率基因打靶开辟了一条全新的思路。这些基N编辑的革命性技术,能大力推动生物学、医学研究的发展。但对这些新技术还没有进行系统研究。文中对基因敲除发展历程、技术原理以及新技术的应用等进行概述,为基因敲除技术的进一步发展应用提供依据。  相似文献   
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[目的]利用TALENs技术定向敲除烟草eIF4E-6基因,为提高优质烤烟品种K326的马铃薯Y病毒(PVY)抗性提供材料。[方法]构建特异靶向烟草eIF4E-基因的TALENs载体,并由农杆菌介导转化K326烟草,用PCR和克隆测序方法筛选目的基因被成功编辑的T_0代转基因烟株。[结果]构建了2个特异靶向eIF4E-6基因的TALENs载体T_3和T_4。2个载体各获得了约40和50株阳性转化的K326 T_0代烟株。T_3载体获得1株目的基因靶位点发生大片段碱基缺失的杂合突变型T_0代单株、3株靶位点发生碱基替换的杂合突变型T_0代单株。T_4载体获得5株靶位点发生碱基替换的杂合突变型T_0代单株。[结论]利用TALENs技术获得的eIF4E-6基因杂合突变型K326 T_0代单株为后续获得纯合基因敲除的K326烟草,并最终获得PVY病毒抗性改良的K326烟草材料奠定了基础。  相似文献   
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