Prevalence of Type VI Secretion System in Spanish Campylobacter jejuni Isolates

Infections from Campylobacter jejuni pose a serious public health problem and are now considered the leading cause of foodborne bacterial gastroenteritis throughout the world. Sequencing of C. jejuni genomes has previously allowed a number of loci to be identified, which encode virulence factors that aid survival and pathogenicity. Recently, a Type VI secretion system (T6SS) consisting of 13 conserved genes was described in C. jejuni strains and recognised to promote pathogenicity and adaptation to the environment. In this study, we determined the presence of this T6SS in 63 Spanish C. jejuni isolates from the food chain and urban effluents using whole‐genome sequencing. Our findings demonstrated that nine (14%) strains harboured the 13 ORFs found in prototype strain C. jejuni 108. Further studies will be necessary to determine the prevalence and importance of T6SS‐positive C. jejuni strains.     

Assessment of insurance coverage and claims in rainfall related risks in processing tomato in Western Spain

An extension of guarantees related to rainfall-related risks in the insurance of processing tomato crops has been accompanied with a large increase in claims in Western Spain, suggesting that damages may have been underestimated in previous years. A database was built by linking agricultural insurance records, meteorological data from local weather stations, and topographic data. The risk of rainfall-related damages in processing tomato in the Extremenian Guadiana river basin (W Spain) was studied using a logistic model. Risks during the growth of the crop and at harvesting were modelled separately. First, the risk related to rainfall was modelled as a function of meteorological, terrain and management variables. The resulting models were used to identify the variables responsible for rainfall-related damages, with a view to assess the potential impact of extending insurance coverage, and to develop an index to express the suitability of the cropping system for insurance. The analyses reveal that damages at different stages of crop development correspond to different hazards. The geographic dependence of the risk influences the scale at which the model might have validity, which together with the year dependency, the possibility of implementing index based insurances is questioned.     

Red deer (Cervus elaphus hispanicus) management in the dehesa system in Central Extremadura,Spain

In this paper we report the results of a study on the suitability of the dehesa (Mediterranean woodlands) as habitat of red deer (Cervus elaphus hispanicus) in a closed country estate of about 4300 ha total surface situated in the San Pedro Mountain, in Central Extremadura, Spain. We have described the deer management with respect to habitat, exploitation mode, deer feeding, population control and production of goods and services. It is concluded that the dehesa system is a rich natural resource that preserves and improves the degraded environment.

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Resumen En el presente trabajo se estudia la dehesa como hábitat del ciervo (Cervus elaphus hispanicus). Se ha tomado como referencia una finca cerrada de 4300 ha de ST situada en la Sierra de San Pedro, en Extremadura, España. Se describen los sistemas de gestión actuales del ciervo en cuanto a hábitat, modo de explotación, alimentación, control de población y producción de bienes y servicios. Concluyendo con la necesidad de fomentar este tipo de explotaciones cinegéticas de dehesa que ayudan a conservar y potenciar el medio ambiente en zonas despobladas y deprimidas

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Abbreviations CS collected surface - DMFCU deedmixed feed cattle units - Fi final inventory - FS fertilized surface - Ii Initial inventory - K Potassium - N Nitrogen - NBF number of breeding females - NBFIi number of breeding females of initial inventory - NBFR number of breeding females released - NBM number of breeding males - NBMIi number of breeding males of initial inventory - NBMR number of breeding males released - NCCR number of captive calves released - NCFi number of calves of final inventory - NCR number of calves released - NFR number of female renovation - NYMR number of young males released - NYFR number of young females released - P phosphorus - PP permanent Pastures - pts pesetas - QS Quercus stalks - RDED resources directly eaten by deer - SPOM selected hunted down poor quality males - SS sowed surface - TN total needs of deer - TNR total number released - UAS useable agrarian surface - WB wooded brush - WQS wooded Quercus surface - WS wooded surface     

Measuring versus estimating net radiation and soil heat flux: Impact on Penman–Monteith reference ET estimates in semiarid regions

The standardized ASCE Penman–Monteith and FAO-56 equations were used to estimate reference evapotranspiration (ET₀) using estimated and measured net radiation (R_n) and soil heat flux (G), based on hourly and daily meteorological data. The estimates were evaluated against lysimeter measurements. The results indicate that using measured or estimated values of R_n and G can have significant effect on the accuracy of the ET₀ estimations, especially when calculations were made on an hourly basis. The FAO-56 version performed very well during the irrigation season on a daily basis. The use of measured R_n and G did not improve ET₀ estimation on a daily basis, therefore, the use of estimated R_n and G appears to be dependable when calculations are based on 24-h weather data. When daily ET₀ was calculated from hourly estimations, the results were different depending on the version used. The ASCE version was more accurate, especially when R_n and G were measured. Therefore, measurement of R_n and G may have potential to improve estimation only when daily ET₀ is calculated from hourly estimations. The PM FAO-56 version was always a little less accurate than the ASCE version. For hourly calculations, using a constant surface resistance (as in FAO-56 version), the PM method underpredicted for high evaporative demand and vice versa. The ASCE version performed better than PM FAO-56 version when R_n and G were measured and estimated. Therefore, ASCE version tended to provide quite accurate values of hourly ET₀, even using estimated values of R_n and G. As conclusion, the methods proposed by FAO-56 for estimating R_n and G tended to produce accurate estimates for daily and hourly ET₀ under semiarid conditions and can be used with some degree of confidence for estimating ET₀. In addition, results suggest that the ASCE standardized equation on an hourly basis improved the accuracy of ET₀ estimation with respect to the FAO-56 version.     

嵌套子集:引入土壤多样性研究的讨论

嵌套子集指在任何特定集合中出现的物种趋向于在更大的集合中也出现,即局部丰富的物种一般分布比较广泛,而局部稀少的物种则分布较窄。本文对嵌套性研究中的有关问题做一评述,并以西班牙的一个研究为范例讨论可以看出,通过数据(土壤类型和土壤集合)试验得出的规律性和生态学上的观点竟非常相似。研究采用了土壤数据集和在计量生态学应用的方法,包括嵌套计算程序(Nested Calcu lator Program)。结果表明:嵌套性在生物群落、土被或土链结构中的性质有相似之处,即地学系统中普遍存在着嵌套性。此方法用于土壤多样性的分析,在有计划的保护生物及土壤资源方面具有现实意义。     

Soil salinity and its distribution determined by soil sampling and electromagnetic techniques

Abstract. Diagnosis of soil salinity and its spatial variability is required to establish control measures in irrigated agriculture. This article shows the usefulness of electromagnetic (EM) and soil sampling techniques to map salinity. We analysed the salinity of a 1‐ha plot of surface‐irrigated olive plantation in Aragon, NE Spain, by measuring the electrical conductivity of the saturation extract (EC_e) of soil samples taken at 22 points, and by reading the Geonics EM38 sensor at 141 points in the horizontal (EM_H) and vertical (EM_V) dipole positions. EM_H and EM_V values had asymmetrical bimodal distributions, with most readings in the non‐saline range and a sharp transition to relatively high readings. Most salinity profiles were uniform (i.e. EM_H=EM_V), except in areas with high salinity and concurrent shallow water tables, where the profiles were inverted as shown by EM_H > EM_V, and by EC_e being greater in shallow than in deeper layers. The regressions of EC_e on EM readings predicted EC_e with R² > 84% for the 0–100 to 0–150 cm soil depths. We then produced salinity contour maps from the 141 EC_e values estimated from the electromagnetic readings and the 22 measured values of EC_e. Owing to the high soil sampling density, the maps were similar (i.e. mean surface‐weighted EC_e values between 3.9 dS m^?1 and 4.2 dS m^?1), although the electromagnetically estimated EC_e improved the mapping of details. Whereas soil sampling is preferred for analysing the vertical distribution of soil salinity, the electromagnetic sensor is ideal for mapping the lateral variability of soil salinity.     

Recent progress in conservation of threatened Spanish vascular flora: a critical review

The transfer of legal responsibilities from the Central Government to the Autonomous Communities ended a tradition of paying little attention to endangered Spanish flora. The studies undertaken to implement the European Natura 2000 network, and to prepare regional ‘Red Data Books’ and lists of protected flora offered a much better view of which species were really at risk. As a consequence, a growing number of botanists is now involved in different aspects of conservation biology. The recent publication of a new Spanish Red List of vascular flora has provided the groundwork for the so-called ‘Threatened Flora Atlas’ project, which will update and homogenise the information on 500 taxa at risk. This project will produce a new Spanish Red Book and monitor the status of 40 selected species. The Spanish strategy for plant conservation is discussed, pointing out some deficiencies, such as the neglect of non-vascular taxa, the uneven and sometimes uncoordinated local policies and the uncertain future of some current initiatives.     

Water Yield and Sediment Yield Simulations for Teba Catchment in Spain Using SWRRB Model: Ⅰ. Model Input and Simulation Experiment

Water yield and sediment yield in the Teba catchment, Spain, were simulated using SWRRB (Simulator for Water Resources in Rural Basins) model. The model is composed of 198 mathematical equations. About 120 items (variables) were input for the simulation, including meteorological and climatic factors, hydrologic factors, topographic factors, parent materials, soils, vegetation, human activities, etc. The simulated results involved surface runoff, subsurface runoff, sediment, peak flow, evapotranspiration, soil water, total biomass,etc. Careful and thorough input data preparation and repeated simulation experiments are the key to get the accurate results. In this work the simulation accuracy for annual water yield prediction reached to 83.68%.``     

The role of small reserves in plant conservation in a region of high diversity in eastern Spain

The Valencian region, in eastern Spain, is home to ≈3050 vascular plant species, 68 of which are listed under the critically endangered IUCN category. To afford protection to our endangered, rare and endemic flora, a network of small (2-20 ha) statutory reserves has been created by the Regional Wildlife Service. These are termed “micro-reserves” and encompass a large number of individual species and natural habitats. The objective is to monitor long term changes in the plant populations and to carry out active management of the protected plants. In the selection of plots, criteria of distinctiveness and endangerment of plants was followed. Eight years after the onset of this initiative, plant micro-reserves have become an essential tool for the effective protection of the diverse flora of this Mediterranean region.