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为了解虎红平板凝集试验(RBT)初筛、试管凝集试验(SAT)确诊并捕杀阳性者对奶牛布氏杆菌病(Brucellosis)的控制效果,2009-2010年,持续监测12个感染程度各异的新建奶牛群。结果表明,当牛群初始感染率较低时,借助RBT与SAT能在较短时期内有效控制并净化布氏杆菌病;但在牛群感染率较高的情况下,上述手段不能有效控制感染扩散。减毒活疫苗模拟感染试验进一步证实,RBT和SAT阳性结果的出现严重滞后于牛群真实感染,这可能是其不能有效防控布氏杆菌病扩散的重要原因。 相似文献
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在无"金标准"的情况下,为评估虎红平板凝集试验(RBT)、荧光偏振分析法(FPA)和间接酶联免疫吸附试验(I-ELISA)用于奶牛布鲁氏菌病的抗体检测能力,作者分别以RBT、FPA、I-ELISA3种方法对采自某奶牛场的血清样品进行检测,通过贝叶斯分析法对检测结果进行分析,研究3种方法检测奶牛布鲁氏菌血清抗体的敏感性(Se)和特异性(Sp)。结果显示,RBT、FPA、I-ELISA3种检测方法的敏感性(Se)分别为91.81%、95.92%和95.98%;特异性(Sp)分别为98.80%、86.54%和98.65%。本研究表明,3种血清学检测方法中以I-ELISA的检测能力最好。 相似文献
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Muma JB Lund A Nielsen K Matope G Munyeme M Mwacalimba K Skjerve E 《Tropical animal health and production》2009,41(5):723-729
The effectiveness of Rose Bengal test (RBT) and fluorescence polarization assay (FPA) in diagnosing cattle brucellosis in
endemic areas was assessed and RBT and FPA test agreement was compared (n = 319). The sensitivity of RBT and FPA in detecting
low Brucella titres were evaluated in paired sera (n = 34). A logistic regression model was constructed to predict cattle test result
in FPA using RBT as the main predictor and incorporating bio-data and animal history. There was 79.3% agreement between the
RBT and FPA (Kappa = 0.59; Std error = 0.05; p = 0.000) and a high correspondence between high RBT scores and positive FPA
results suggesting that sera with high RBT score may not require confirmation with tests such as competitive-ELISA or CFT.
High FPA cut-off points were more likely to miss animals with low antibody titres. The RBT had a reduced ability in detecting
low antibody titres compared to the FPA. FPA test interpretation was improved if a priori information, such as sex and age was used. Under the challenging disease surveillance conditions prevailing in rural Africa,
field-testing methods that are sensitive and specific; allow single animal contact, low technical skills in data interpretation
are suitable. 相似文献
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采用虎红平板凝集试验(RBT)、全乳环状试验(MRT)和试管凝集试验(SAT)3种国家标准认可的动物布病检疫方法检测奶牛布氏杆菌抗体,并进行了比较研究。从82头非免疫和S2菌苗免疫的奶牛采集血样和乳样,RBT检测血样的阳性率为51.22%(42/82),MRT检测乳样的阳性率为46.34%(38/82),SAT检测血样的阳性率为45.12%(37/82)。以SAT定量检测的结果为参照标准,RBT初筛试验的符合率比MRT高,而且MRT反应强度的差异与SAT抗体滴度没有明显相关性。另对乳清和血清样本中布氏杆菌抗体的差异进行了分析讨论。 相似文献
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为评价布鲁氏菌病补体结合酶联免疫吸附试验抗体检测试剂盒( CF-ELISA试剂盒)的敏感性、特异性及与其他试剂盒的符合率,用CF-ELISA试剂盒对布病感染地区牛、羊血清各200份,布病净化地区牛、羊群血清各100份进行抗体检测,同时与其他商品化检测试剂进行了比较。结果表明,感染地区牛、羊血清抗体的McNemar检验结果表明CF-ELISA试剂盒与间接酶联免疫吸附试验( iELISA)试剂盒和CGS的敏感性和特异性差异均不显著( P>0.05),Kappa一致性检验分析结果表明,CF-ELISA试剂盒与iELISA试剂盒检测结果有高度的符合性。检测净化地区牛、羊群血清抗体产品间的特异性和符合率均为100%。 相似文献
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