Pet birds as potential reservoirs of virulent and antibiotic resistant zoonotic bacteria

Bacterial pathogens carried by pet birds are considered a risk for birds, workers, and pet owners. This study investigated the potential of pet birds as reservoirs for virulent multidrug-resistant (MDR) zoonotic bacteria and assessed the genetic relatedness and diversity of bacterial isolates from pet birds and human contacts. Cloacal and tracheal swabs from 125 pet birds and 70 hand swabs from human contacts were collected. The results revealed that the pet birds were reservoirs for Escherichia coli, Klebsiella pneumoniae (17.6 %, each), and Staphylococcus aureus (15.2 %). These isolates were also identified in their human contacts, at percentages of 14.3 %, 12.9 %, and 24.3 %, respectively. Virulence associated genes were identified from E. coli (stx2, stx2f, eaeA, and hlyA), K. pneumoniae (fimH, TraT, and magA), and S. aureus (PVL, hly, sea, sed genes) isolates. Multidrug-resistant E. coli, K. pneumoniae, and S. aureus were highly prevalent (81.3 %, 90.3 %, and 61.1 %, respectively). The genetic relationship between the E. coli and K. pneumoniae isolates from the pet birds and human contacts were determined by ERIC-PCR, while, RAPD-PCR was used for the S. aureus isolates. ERIC-PCR was found to have the highest discriminatory power. The clustering of the isolates from the pet birds and human contacts indicated potential transmission between the birds and workers. In conclusion, pet birds could act as potential reservoirs for zoonotic bacterial pathogens; thus, posing a risk to their human contacts.     

《宠物外科手术学》教学模式改革与探讨

《宠物外科手术学》是一门实践性很强的学科,与其他专业相比,更强调能力的培养,强调实验技能和临床技能的训练,是宠物相关专业技能培养的重要组成部分。该课程对培养学生的临床思维能力、合理运用手术基本操作技术能力、学生对宠物外科手术技能综合运用能力、学生的社会就业方向等有着积极而重要的作用。为此,如何给学生更好地传授理论知识,提供更多临床实践和动手能力的机会,需要学校和教师在人才培养方面不断思索和改革。通过多年的临床教学经验,对该课程进行了一些教学改革尝试,旨在更好地培养学生宠物外科手术的实践能力,以适应社会的需要与发展。     

职业教育国家教学资源库建设与实践——以宠物内科病课程为例

以现代宠物技术专业教学资源库建设为背景,以宠物内科病课程资源库建设为个案,总结了教学资源库的建设思路、步骤、内容及资源库应用推广情况,以建立优质、高效的共享型教育资源库,推进教学质量的全面提升。     

锦州地区宠物犬寄生虫流行区系及流行病学调查研究

随机抽检锦州地区10家宠物门诊的50只宠物犬,用漂浮法和沉淀法检查粪便中的寄生虫卵,鉴定其种类,对其感染率和感染强度进行统计,最后确定优势虫种,并对其流行病学进行研究。结果发现,锦州地区宠物犬寄生虫有11种,其中优势虫种为犬弓首蛔虫、犬钩虫、犬复孔绦虫、疥螨和蠕形螨。通过现场考查及查阅有关资料确定了优势虫种流行病学的规律和特点,为锦州地区宠物犬寄生虫病的防治工作提供了科学依据。     

Molecular characterisation of Cryptosporidium isolates from pet reptiles

In this study, we investigated the presence of Cryptosporidium in 171 faecal samples from reptiles commonly used as pet animals. These include lizards belonging to the genera Eublepharis, Pogona, Chlamydosaurus, Hemiteconyx, Teratoscincus, Tiliqua, Iguana, and Chamaeleo, snakes of the genera Lampropeltis, Elaphe, Python, Boa and Corallus, and tortoises belonging to the genera Testudo and Kinixys. Cryptosporidium oocysts were detected by immunofluorescence using a commercially available kit and cryptosporidial DNA by amplification of a polymorphic fragment of the 18S rDNA and the HSP70 locus.Cryptosporidium was detected in 38.6% and 25.1% of the samples analysed by immunofluorescence and PCR, respectively. Molecular characterisation of the isolates confirmed that C. serpentis and C. varanii (syn. C. saurophilum) are the main species involved in infection in pet reptiles but also showed the presence of C. parvum and C. muris, as well as other species or genotypes of this parasite including the Cryptosporidium mouse genotype and Cryptosporidium tortoise genotype previously described in reptiles. In addition, a Cryptosporidium sp. was isolated from a chameleon and a python.     

家庭宠物对弓形虫病流行的影响

随着经济的发展,家庭盛行豢养宠物已经成为一种时尚,与宠物有关的寄生虫病亦随之而来,人群感染弓形虫病的比率逐年上升,弓形虫的潜在危害直接影响着人民的身体健康。如何正确认识弓形虫对人类的危害,对疾病预防和控制有着重要的意义。