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为明确陕西省小麦不同部位镰刀菌种群结构及其毒素化学型,于2022年小麦灌浆期分别从陕西省渭南市、宝鸡市、咸阳市、西安市采集小麦穗部、茎秆以及茎基部具有明显病状的样品,采用单孢分离法获得镰刀菌菌株,并使用分子生物学鉴定其种群结构及毒素化学型。结果表明,从采集的样本中共分离到156株镰刀菌菌株,其中发生在穗部的赤霉病以及发生在茎秆的秆腐病的优势病原菌均为禾谷镰刀菌(Fusarium graminearum),主要以15-ADON化学型为主;发生在茎基部的茎基腐病的优势病原菌为假禾谷镰刀菌(F.pseudograminearum),其15-ADON和3-ADON化学型占比差异不大;未检测到NIV毒素化学型的镰刀菌。以上结果说明,小麦不同部位对镰刀菌毒素化学型可能不存在选择性,小麦茎基部与穗部和茎秆的镰刀菌毒素化学型不同,原因可能是由优势病原菌不同造成的。 相似文献
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A mechanistic weather-driven model was developed based on the infection cycle of Aspergillus flavus on maize to predict the risk of aflatoxin contamination in field on a daily basis from silk emergence to harvest; hourly data of temperature, relative humidity and rain were used as model input. The work was done in four steps: (i) development of the model prototype; (ii) collection of Italian field data on aflatoxin contamination in maize with related crop and weather data; (iii) development of a probability index to exceed the legal limit of 5 μg of aflatoxin B1 per kg of unprocessed maize by combining model predictions and field data in a logistic regression; and (iv) validation with Italian data of the probability index and release of the predictive model, named AFLA-maize. Predictions of maize contamination above the threshold of 5 μg/kg in the data set used for parameterization of the regression equation were correct for 73% of field samples; 59% and 14%, respectively, were not contaminated and contaminated. In a second independent data set, 68% of samples were correctly predicted. The model AFLA-maize provides prediction of A. flavus infection and aflatoxin contamination along the growing season and at harvest. This information is useful to support decision-making for (i) crop management, (ii) harvest timing, (iii) maize lots cleaning and logistic, and (iv) maize sampling for aflatoxin analysis at consignment. 相似文献
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为明确贮藏时间对饲用燕麦种子中寄藏真菌和真菌毒素的影响,本研究通过平板培养法、形态学观察和rDNA-ITS序列分析法,对3个燕麦(Avena sativa)品种种子携带的真菌进行了分离鉴定;通过高效液相色谱质谱联用法对优势菌属产生的毒性较强的3种真菌毒素细交链孢酮酸(TeA)、黄曲霉毒素B1(AFB1)和桔青霉素(CIT)进行了检测和分析。结果表明:饲用燕麦种子的带菌量随贮藏时间的增加总体呈降低趋势。从供试材料中共检出真菌16属34种,优势属为链格孢菌属、曲霉菌属和青霉菌属;带菌种类和分离率随贮藏年限的变化差异显著,不同品种的带菌情况也差异显著;3种真菌毒素随贮藏年限的延长基本呈先升后降的趋势,贮藏年限和品种间的互作对毒素含量的影响最大。综上,贮藏时间对饲用燕麦种子的带菌情况和真菌毒素检出量影响显著。 相似文献
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Aspergillus section Flavi is able to produce aflatoxins (AFs) in vitro down to 0.85 aw with a potential maximum occurring between 0.95 and 0.99 aw, while in the field AFs increased significantly with kernel humidity below 0.95 aw. In order to clarify this apparent discrepancy, a 3-year field trial with artificial inoculation of maize ears with Aspergillus flavus strains was organised. The co-occurrence of Aspergillus section Flavi and Gibberella fujikuroi species complex (Gfsc) was observed. The incidence of A. flavus was significantly influenced by the year and negatively related to Gfsc incidence. In 2012, when the highest temperature and the lowest rain were registered, aflatoxin B1 (AFB1) content was the highest and aw < 0.95 in kernels was measured early throughout the growing season. In 2013 and 2014, the temperature was lower and rain more abundant, and aw decreased below 0.95 only close to harvest and AFB1 contamination was limited. The possibility of describing/predicting reasonably well aw dynamic based on temperature, (degree day) was confirmed. With aw > 0.95, a positive correlation between AFB1 production rate and aw was found, but a negative correlation resulted with aw < 0.95. Other factors than aw play a role, but aw = 0.95 should be considered as an indicator of suitable conditions for rapid aflatoxin accumulation. 相似文献
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为了建立一种准确、快速鉴定赤霉菌毒素的方法,根据赤霉菌毒素生物合成调控路径T ri5-T ri6基因保守序列,设计了一对通用引物,用于检测和鉴别产生脱氧雪腐镰刀菌烯醇(DON)和雪腐镰刀菌烯醇(N IV)毒素的赤霉菌特异DNA片断。结果表明,产生毒素DON的赤霉菌具有一个300 bp的特异DNA片断,而产生毒素N IV的则为360 bp。PCR检测与HPLC或GC/M S化学分析结果完全一致。利用这一方法分析检测了小麦、玉米籽粒中赤霉菌产毒类型,发现除健康籽粒外,在所有轻微、中度、严重感染赤霉病的小麦或玉米籽粒中,均同时检测到DON及N IV的两种DNA片断,表明这一对通用引物对两种毒素特异DNA片断的扩增效率相同。这些结果说明,该技术是一种经济、快速、可靠的PCR检测技术,可广泛用于赤霉菌及其毒素检测鉴定中。 相似文献
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研究5种霉菌毒素吸附剂对蛋鸡生产性能和蛋品质的影响。选用360日龄海兰灰产蛋鸡864只,随机分为6个处理,每处理4个重复,对照组饲喂基础日粮,处理1~5组在基础日粮的基础上分别添加5种霉菌毒素吸附剂A、B、C、D和E,试验期56 d。结果表明,霉菌毒素吸附剂A、C、D和E对产蛋鸡生产性能没有影响,吸附剂B能够提高产蛋鸡产蛋率(P<0.05)。5种霉菌毒素吸附剂对第35天鸡蛋蛋壳厚度、哈氏单位、蛋黄比率和蛋壳强度没有影响(P>0.05),添加霉菌毒素吸附剂产品C、D、E组的蛋黄色泽分别比对照组高43.64%、36.83%和43.28%(P<0.05)。第56天时,日粮中添加吸附剂A处理组的蛋黄色泽显著低于对照组(P<0.05),添加吸附剂C和E处理组的蛋黄色泽分别比对照组高10.39%和9.85%(P<0.05);添加5种不同霉菌毒素吸附剂处理组的哈氏单位和蛋壳强度均低于对照组(P<0.05)。因此,霉菌毒素吸附剂产品B能够提高蛋鸡产蛋率(P<0.05);长期使用霉菌毒素吸附剂对鸡蛋品质有一定影响,主要表现为蛋壳强度和哈氏单位降低。 相似文献
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